Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00050 and PGA1_78p00060 are separated by 166 nucleotides PGA1_78p00060 and PGA1_78p00070 are separated by 83 nucleotides PGA1_78p00070 and PGA1_78p00080 overlap by 4 nucleotides
PGA1_78p00050: PGA1_78p00050 - peroxidase/catalase HPI, at 5,749 to 7,917
_78p00050
PGA1_78p00060: PGA1_78p00060 - Uncharacterized protein conserved in bacteria, at 8,084 to 8,437
_78p00060
PGA1_78p00070: PGA1_78p00070 - 'RNA polymerase sigma-70, ECF subfamily; ECF26 subgroup', at 8,521 to 9,078
_78p00070
PGA1_78p00080: PGA1_78p00080 - Predicted transmembrane transcriptional regulator (anti-sigma factor), at 9,075 to 9,917
_78p00080
Position (kb)
8
9 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 7.158 kb on - strand, within PGA1_78p00050 at 7.178 kb on + strand, within PGA1_78p00050 at 7.186 kb on - strand at 7.270 kb on + strand, within PGA1_78p00050 at 7.280 kb on + strand, within PGA1_78p00050 at 7.338 kb on - strand, within PGA1_78p00050 at 8.064 kb on + strand at 8.064 kb on + strand at 8.067 kb on - strand at 8.112 kb on + strand at 8.145 kb on - strand, within PGA1_78p00060 at 8.200 kb on - strand, within PGA1_78p00060 at 8.200 kb on - strand, within PGA1_78p00060 at 8.207 kb on - strand, within PGA1_78p00060 at 8.887 kb on + strand, within PGA1_78p00070 at 9.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 7,158 - PGA1_78p00050 0.65 +0.7 7,178 + PGA1_78p00050 0.66 +0.3 7,186 - -2.0 7,270 + PGA1_78p00050 0.70 +0.0 7,280 + PGA1_78p00050 0.71 -0.7 7,338 - PGA1_78p00050 0.73 -1.7 8,064 + -0.3 8,064 + -1.3 8,067 - +0.5 8,112 + +0.3 8,145 - PGA1_78p00060 0.17 -0.3 8,200 - PGA1_78p00060 0.33 +1.9 8,200 - PGA1_78p00060 0.33 -2.3 8,207 - PGA1_78p00060 0.35 -1.3 8,887 + PGA1_78p00070 0.66 -3.0 9,072 + +2.3
Or see this region's nucleotide sequence