Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01040 and PGA1_262p01050 are separated by 528 nucleotides PGA1_262p01050 and PGA1_262p01060 are separated by 117 nucleotides PGA1_262p01060 and PGA1_262p01070 are separated by 103 nucleotides
PGA1_262p01040: PGA1_262p01040 - periplasmic dipeptide transport protein precursor (dipeptide-binding protein) (DBP), at 113,738 to 115,294
_262p01040
PGA1_262p01050: PGA1_262p01050 - Hemolysin-type calcium-binding repeat (2 copies)., at 115,823 to 116,746
_262p01050
PGA1_262p01060: PGA1_262p01060 - Protein of unknown function (DUF995)., at 116,864 to 117,352
_262p01060
PGA1_262p01070: PGA1_262p01070 - tRNA-Arg, at 117,456 to 117,532
_262p01070
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 114.906 kb on + strand, within PGA1_262p01040 at 114.951 kb on - strand, within PGA1_262p01040 at 115.033 kb on + strand, within PGA1_262p01040 at 115.033 kb on - strand, within PGA1_262p01040 at 115.158 kb on + strand at 115.166 kb on - strand at 115.166 kb on - strand at 115.352 kb on + strand at 115.477 kb on - strand at 115.524 kb on - strand at 115.637 kb on + strand at 115.722 kb on - strand at 115.846 kb on + strand at 115.846 kb on + strand at 115.854 kb on - strand at 115.857 kb on + strand at 115.912 kb on + strand at 115.938 kb on + strand, within PGA1_262p01050 at 115.946 kb on - strand, within PGA1_262p01050 at 115.948 kb on + strand, within PGA1_262p01050 at 115.956 kb on - strand, within PGA1_262p01050 at 116.090 kb on - strand, within PGA1_262p01050 at 116.090 kb on - strand, within PGA1_262p01050 at 116.114 kb on - strand, within PGA1_262p01050 at 116.137 kb on - strand, within PGA1_262p01050 at 116.191 kb on - strand, within PGA1_262p01050 at 116.250 kb on - strand, within PGA1_262p01050 at 116.331 kb on - strand, within PGA1_262p01050 at 116.342 kb on + strand, within PGA1_262p01050 at 116.371 kb on + strand, within PGA1_262p01050 at 116.398 kb on + strand, within PGA1_262p01050 at 116.406 kb on - strand, within PGA1_262p01050 at 116.406 kb on - strand, within PGA1_262p01050 at 116.622 kb on + strand, within PGA1_262p01050 at 116.622 kb on + strand, within PGA1_262p01050 at 116.732 kb on - strand at 116.839 kb on + strand at 116.839 kb on + strand at 116.839 kb on + strand at 116.847 kb on - strand at 116.849 kb on + strand at 116.895 kb on + strand at 116.994 kb on - strand, within PGA1_262p01060 at 117.169 kb on - strand, within PGA1_262p01060 at 117.254 kb on - strand, within PGA1_262p01060 at 117.288 kb on - strand, within PGA1_262p01060 at 117.295 kb on - strand, within PGA1_262p01060 at 117.295 kb on - strand, within PGA1_262p01060 at 117.331 kb on - strand at 117.647 kb on + strand at 117.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 114,906 + PGA1_262p01040 0.75 -0.6 114,951 - PGA1_262p01040 0.78 +0.8 115,033 + PGA1_262p01040 0.83 -0.7 115,033 - PGA1_262p01040 0.83 -0.3 115,158 + +0.6 115,166 - +1.8 115,166 - +0.8 115,352 + -0.8 115,477 - -0.4 115,524 - -1.8 115,637 + +0.6 115,722 - -0.0 115,846 + -0.6 115,846 + +0.0 115,854 - -0.5 115,857 + +2.0 115,912 + +1.1 115,938 + PGA1_262p01050 0.12 +0.0 115,946 - PGA1_262p01050 0.13 -1.2 115,948 + PGA1_262p01050 0.14 -1.8 115,956 - PGA1_262p01050 0.14 +0.8 116,090 - PGA1_262p01050 0.29 +0.2 116,090 - PGA1_262p01050 0.29 +0.2 116,114 - PGA1_262p01050 0.31 +0.0 116,137 - PGA1_262p01050 0.34 -0.3 116,191 - PGA1_262p01050 0.40 -1.1 116,250 - PGA1_262p01050 0.46 -0.8 116,331 - PGA1_262p01050 0.55 +0.9 116,342 + PGA1_262p01050 0.56 -0.1 116,371 + PGA1_262p01050 0.59 -0.9 116,398 + PGA1_262p01050 0.62 -1.1 116,406 - PGA1_262p01050 0.63 -1.4 116,406 - PGA1_262p01050 0.63 +0.2 116,622 + PGA1_262p01050 0.86 -0.1 116,622 + PGA1_262p01050 0.86 +0.5 116,732 - -1.2 116,839 + -0.5 116,839 + -0.7 116,839 + -0.6 116,847 - +1.8 116,849 + -0.9 116,895 + +0.4 116,994 - PGA1_262p01060 0.27 -0.1 117,169 - PGA1_262p01060 0.62 -0.2 117,254 - PGA1_262p01060 0.80 +1.8 117,288 - PGA1_262p01060 0.87 +0.4 117,295 - PGA1_262p01060 0.88 +0.9 117,295 - PGA1_262p01060 0.88 -0.4 117,331 - +0.4 117,647 + +0.8 117,746 - -0.3
Or see this region's nucleotide sequence