Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32820 and PGA1_c32830 are separated by 64 nucleotides PGA1_c32830 and PGA1_c32840 are separated by 33 nucleotides PGA1_c32840 and PGA1_c32850 are separated by 53 nucleotides PGA1_c32850 and PGA1_c32860 are separated by 71 nucleotides
PGA1_c32820: PGA1_c32820 - peptidase, M16 family, at 3,442,914 to 3,444,338
_c32820
PGA1_c32830: PGA1_c32830 - hypothetical protein, at 3,444,403 to 3,444,600
_c32830
PGA1_c32840: PGA1_c32840 - Protein of unknown function (DUF3035)., at 3,444,634 to 3,445,149
_c32840
PGA1_c32850: PGA1_c32850 - putative lipoprotein signal peptidase, at 3,445,203 to 3,445,685
_c32850
PGA1_c32860: PGA1_c32860 - bifunctional purine biosynthesis protein PurH, at 3,445,757 to 3,447,346
_c32860
Position (kb)
3445
3446 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3444.208 kb on - strand at 3444.219 kb on - strand at 3444.261 kb on + strand at 3444.320 kb on + strand at 3444.388 kb on + strand at 3444.451 kb on - strand, within PGA1_c32830 at 3444.458 kb on - strand, within PGA1_c32830 at 3444.458 kb on - strand, within PGA1_c32830 at 3444.462 kb on - strand, within PGA1_c32830 at 3444.557 kb on - strand, within PGA1_c32830 at 3444.569 kb on - strand, within PGA1_c32830 at 3444.616 kb on + strand at 3444.616 kb on + strand at 3444.657 kb on - strand at 3444.657 kb on - strand at 3444.751 kb on - strand, within PGA1_c32840 at 3444.752 kb on + strand, within PGA1_c32840 at 3444.760 kb on - strand, within PGA1_c32840 at 3444.772 kb on + strand, within PGA1_c32840 at 3444.858 kb on + strand, within PGA1_c32840 at 3444.859 kb on - strand, within PGA1_c32840 at 3444.898 kb on - strand, within PGA1_c32840 at 3444.940 kb on - strand, within PGA1_c32840 at 3444.952 kb on - strand, within PGA1_c32840 at 3444.974 kb on + strand, within PGA1_c32840 at 3444.974 kb on + strand, within PGA1_c32840 at 3444.982 kb on - strand, within PGA1_c32840 at 3445.001 kb on - strand, within PGA1_c32840 at 3445.021 kb on - strand, within PGA1_c32840 at 3445.062 kb on - strand, within PGA1_c32840 at 3445.125 kb on - strand at 3445.153 kb on + strand at 3445.187 kb on + strand at 3445.195 kb on - strand at 3445.195 kb on - strand at 3445.195 kb on - strand at 3445.195 kb on - strand at 3445.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 3,444,208 - +0.0 3,444,219 - -0.9 3,444,261 + +0.8 3,444,320 + -1.2 3,444,388 + +0.5 3,444,451 - PGA1_c32830 0.24 -0.0 3,444,458 - PGA1_c32830 0.28 -0.6 3,444,458 - PGA1_c32830 0.28 +0.5 3,444,462 - PGA1_c32830 0.30 +0.8 3,444,557 - PGA1_c32830 0.78 +0.0 3,444,569 - PGA1_c32830 0.84 -0.0 3,444,616 + +0.3 3,444,616 + -0.5 3,444,657 - -0.7 3,444,657 - -0.5 3,444,751 - PGA1_c32840 0.23 -2.3 3,444,752 + PGA1_c32840 0.23 -0.8 3,444,760 - PGA1_c32840 0.24 -3.0 3,444,772 + PGA1_c32840 0.27 -2.5 3,444,858 + PGA1_c32840 0.43 +0.2 3,444,859 - PGA1_c32840 0.44 -2.2 3,444,898 - PGA1_c32840 0.51 -3.1 3,444,940 - PGA1_c32840 0.59 -2.2 3,444,952 - PGA1_c32840 0.62 +0.0 3,444,974 + PGA1_c32840 0.66 -2.2 3,444,974 + PGA1_c32840 0.66 -1.4 3,444,982 - PGA1_c32840 0.67 -2.6 3,445,001 - PGA1_c32840 0.71 -1.7 3,445,021 - PGA1_c32840 0.75 -2.4 3,445,062 - PGA1_c32840 0.83 -2.5 3,445,125 - -1.3 3,445,153 + -0.5 3,445,187 + -1.2 3,445,195 - -3.1 3,445,195 - -1.3 3,445,195 - -3.1 3,445,195 - -1.6 3,445,208 - -0.8
Or see this region's nucleotide sequence