Experiment: PYE 0.5x 30C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02019 and CCNA_02020 are separated by 82 nucleotides CCNA_02020 and CCNA_03961 are separated by 10 nucleotides CCNA_03961 and CCNA_02021 overlap by 4 nucleotides CCNA_02021 and CCNA_02022 overlap by 1 nucleotides
CCNA_02019: CCNA_02019 - NADH-quinone oxidoreductase chain J, at 2,166,636 to 2,167,253
_02019
CCNA_02020: CCNA_02020 - NADH-quinone oxidoreductase chain I, at 2,167,336 to 2,167,827
_02020
CCNA_03961: CCNA_03961 - hypothetical protein, at 2,167,838 to 2,168,062
_03961
CCNA_02021: CCNA_02021 - hypothetical protein, at 2,168,059 to 2,168,298
_02021
CCNA_02022: CCNA_02022 - NADH-quinone oxidoreductase chain H, at 2,168,298 to 2,169,368
_02022
Position (kb)
2167
2168
2169 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2167.844 kb on - strand at 2167.877 kb on - strand, within CCNA_03961 at 2167.877 kb on - strand, within CCNA_03961 at 2167.877 kb on - strand, within CCNA_03961 at 2167.931 kb on + strand, within CCNA_03961 at 2167.964 kb on - strand at 2168.108 kb on + strand, within CCNA_02021 at 2168.111 kb on - strand, within CCNA_02021 at 2168.114 kb on + strand, within CCNA_02021 at 2168.122 kb on - strand, within CCNA_02021 at 2168.122 kb on - strand, within CCNA_02021 at 2168.143 kb on + strand, within CCNA_02021 at 2168.151 kb on - strand, within CCNA_02021 at 2168.158 kb on + strand, within CCNA_02021 at 2168.158 kb on - strand, within CCNA_02021 at 2168.210 kb on + strand, within CCNA_02021 at 2168.218 kb on - strand, within CCNA_02021
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 30C survival rep2 12 days remove 2,167,844 - +1.5 2,167,877 - CCNA_03961 0.17 +1.4 2,167,877 - CCNA_03961 0.17 +1.2 2,167,877 - CCNA_03961 0.17 +2.1 2,167,931 + CCNA_03961 0.41 -0.4 2,167,964 - +1.6 2,168,108 + CCNA_02021 0.20 +2.2 2,168,111 - CCNA_02021 0.22 +1.4 2,168,114 + CCNA_02021 0.23 -0.4 2,168,122 - CCNA_02021 0.26 +3.2 2,168,122 - CCNA_02021 0.26 +0.5 2,168,143 + CCNA_02021 0.35 +0.7 2,168,151 - CCNA_02021 0.38 +0.7 2,168,158 + CCNA_02021 0.41 +1.8 2,168,158 - CCNA_02021 0.41 +1.4 2,168,210 + CCNA_02021 0.63 +0.8 2,168,218 - CCNA_02021 0.66 +0.9
Or see this region's nucleotide sequence