Experiment: PYE 0.5x 30C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00006 and CCNA_00007 are separated by 149 nucleotides CCNA_00007 and CCNA_00008 are separated by 400 nucleotides CCNA_00008 and CCNA_00009 are separated by 61 nucleotides
CCNA_00006: CCNA_00006 - enoyl-CoA hydratase, at 4,022 to 4,810
_00006
CCNA_00007: CCNA_00007 - SSU ribosomal protein S20P, at 4,960 to 5,235
_00007
CCNA_00008: CCNA_00008 - chromosome replication initiator protein DnaA, at 5,636 to 7,108
_00008
CCNA_00009: CCNA_00009 - alkylated DNA repair protein AlkB, at 7,170 to 7,790
_00009
Position (kb) 5
6
7
8 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 4.661 kb on + strand, within CCNA_00006 at 4.666 kb on + strand, within CCNA_00006 at 4.674 kb on - strand, within CCNA_00006 at 4.688 kb on + strand, within CCNA_00006 at 4.783 kb on + strand at 4.783 kb on + strand at 4.783 kb on + strand at 4.783 kb on - strand at 4.791 kb on - strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.795 kb on + strand at 4.803 kb on - strand at 4.803 kb on - strand at 4.803 kb on - strand at 4.815 kb on + strand at 4.863 kb on + strand at 5.471 kb on + strand at 5.471 kb on + strand at 5.471 kb on + strand at 5.514 kb on - strand at 5.598 kb on + strand at 7.124 kb on + strand at 7.137 kb on - strand at 7.139 kb on + strand at 7.185 kb on - strand at 7.193 kb on + strand at 7.198 kb on + strand at 7.227 kb on + strand at 7.279 kb on + strand, within CCNA_00009 at 7.287 kb on - strand, within CCNA_00009 at 7.299 kb on - strand, within CCNA_00009 at 7.354 kb on + strand, within CCNA_00009 at 7.359 kb on + strand, within CCNA_00009 at 7.367 kb on - strand, within CCNA_00009 at 7.377 kb on - strand, within CCNA_00009 at 7.384 kb on + strand, within CCNA_00009 at 7.440 kb on - strand, within CCNA_00009 at 7.459 kb on + strand, within CCNA_00009 at 7.467 kb on - strand, within CCNA_00009 at 7.467 kb on - strand, within CCNA_00009 at 7.497 kb on + strand, within CCNA_00009 at 7.497 kb on + strand, within CCNA_00009 at 7.505 kb on - strand at 7.505 kb on - strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.507 kb on + strand, within CCNA_00009 at 7.526 kb on - strand, within CCNA_00009 at 7.548 kb on - strand, within CCNA_00009 at 7.548 kb on - strand, within CCNA_00009 at 7.566 kb on + strand, within CCNA_00009 at 7.603 kb on - strand, within CCNA_00009 at 7.622 kb on + strand, within CCNA_00009 at 7.643 kb on + strand, within CCNA_00009 at 7.657 kb on + strand, within CCNA_00009 at 7.657 kb on + strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.665 kb on - strand, within CCNA_00009 at 7.692 kb on + strand, within CCNA_00009 at 7.692 kb on + strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.700 kb on - strand, within CCNA_00009 at 7.742 kb on - strand at 7.758 kb on + strand at 7.759 kb on - strand at 7.759 kb on - strand at 7.763 kb on + strand at 7.770 kb on + strand at 7.796 kb on - strand at 7.807 kb on + strand at 7.930 kb on + strand at 7.930 kb on + strand at 7.931 kb on + strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.938 kb on - strand at 7.939 kb on - strand at 7.939 kb on - strand at 7.958 kb on - strand at 7.989 kb on - strand at 7.999 kb on + strand at 8.002 kb on - strand at 8.039 kb on + strand at 8.039 kb on + strand at 8.047 kb on - strand at 8.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 30C survival rep2 12 days remove 4,661 + CCNA_00006 0.81 -1.6 4,666 + CCNA_00006 0.82 -1.3 4,674 - CCNA_00006 0.83 -1.3 4,688 + CCNA_00006 0.84 -2.3 4,783 + -1.4 4,783 + -0.7 4,783 + -1.0 4,783 - -2.0 4,791 - -2.6 4,795 + -1.5 4,795 + -2.3 4,795 + -1.8 4,795 + -1.0 4,795 + -2.0 4,795 + -1.2 4,795 + -0.8 4,795 + -1.5 4,803 - +1.0 4,803 - -0.8 4,803 - +1.0 4,815 + +0.7 4,863 + +0.9 5,471 + +2.3 5,471 + +1.1 5,471 + +1.6 5,514 - +1.0 5,598 + -0.4 7,124 + +1.1 7,137 - -0.6 7,139 + +0.8 7,185 - +0.0 7,193 + +1.2 7,198 + +0.6 7,227 + +0.2 7,279 + CCNA_00009 0.18 -0.2 7,287 - CCNA_00009 0.19 -1.1 7,299 - CCNA_00009 0.21 -0.7 7,354 + CCNA_00009 0.30 -1.6 7,359 + CCNA_00009 0.30 +1.0 7,367 - CCNA_00009 0.32 +0.7 7,377 - CCNA_00009 0.33 +0.2 7,384 + CCNA_00009 0.34 -0.6 7,440 - CCNA_00009 0.43 +0.7 7,459 + CCNA_00009 0.47 +1.3 7,467 - CCNA_00009 0.48 +0.9 7,467 - CCNA_00009 0.48 -1.8 7,497 + CCNA_00009 0.53 +0.7 7,497 + CCNA_00009 0.53 -1.2 7,505 - +4.3 7,505 - CCNA_00009 0.54 +1.1 7,507 + CCNA_00009 0.54 -0.7 7,507 + CCNA_00009 0.54 +0.5 7,507 + CCNA_00009 0.54 -0.2 7,526 - CCNA_00009 0.57 +0.9 7,548 - CCNA_00009 0.61 -2.4 7,548 - CCNA_00009 0.61 -0.6 7,566 + CCNA_00009 0.64 -1.3 7,603 - CCNA_00009 0.70 -1.9 7,622 + CCNA_00009 0.73 -1.2 7,643 + CCNA_00009 0.76 -2.5 7,657 + CCNA_00009 0.78 -0.3 7,657 + CCNA_00009 0.78 +1.0 7,665 - CCNA_00009 0.80 -1.1 7,665 - CCNA_00009 0.80 +0.6 7,665 - CCNA_00009 0.80 +0.5 7,665 - CCNA_00009 0.80 +1.1 7,692 + CCNA_00009 0.84 -0.4 7,692 + CCNA_00009 0.84 +0.9 7,700 - CCNA_00009 0.85 -0.1 7,700 - CCNA_00009 0.85 +0.1 7,700 - CCNA_00009 0.85 +0.9 7,742 - +0.8 7,758 + -0.1 7,759 - +0.1 7,759 - -1.9 7,763 + +0.3 7,770 + -0.8 7,796 - -1.0 7,807 + -0.8 7,930 + -1.8 7,930 + -0.4 7,931 + +0.1 7,938 - -1.0 7,938 - +0.4 7,938 - -0.7 7,938 - -0.6 7,939 - -0.8 7,939 - -0.9 7,958 - -5.4 7,989 - -5.5 7,999 + -5.6 8,002 - +2.0 8,039 + -2.2 8,039 + -1.4 8,047 - -3.7 8,055 - -2.1
Or see this region's nucleotide sequence