Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides BWI76_RS20665 and BWI76_RS20670 are separated by 82 nucleotides BWI76_RS20670 and BWI76_RS20675 are separated by 14 nucleotides BWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotides
BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424
_RS20660
BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287
_RS20665
BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668
_RS20670
BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390
_RS20675
BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840
_RS20680
Position (kb)
4324
4325
4326 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4323.795 kb on + strand, within BWI76_RS20665 at 4323.959 kb on + strand, within BWI76_RS20665 at 4323.967 kb on - strand, within BWI76_RS20665 at 4324.019 kb on + strand, within BWI76_RS20665 at 4324.114 kb on - strand, within BWI76_RS20665 at 4324.152 kb on + strand, within BWI76_RS20665 at 4324.189 kb on + strand, within BWI76_RS20665 at 4324.220 kb on + strand at 4324.515 kb on + strand, within BWI76_RS20670 at 4324.533 kb on + strand, within BWI76_RS20670 at 4325.054 kb on - strand, within BWI76_RS20670 at 4325.140 kb on + strand, within BWI76_RS20670 at 4325.549 kb on + strand at 4325.701 kb on - strand at 4325.726 kb on + strand at 4325.731 kb on + strand at 4325.779 kb on - strand, within BWI76_RS20675 at 4325.864 kb on + strand, within BWI76_RS20675 at 4325.904 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand at 4326.258 kb on - strand, within BWI76_RS20675 at 4326.315 kb on + strand, within BWI76_RS20675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 4,323,795 + BWI76_RS20665 0.43 +0.6 4,323,959 + BWI76_RS20665 0.62 +0.1 4,323,967 - BWI76_RS20665 0.63 -0.8 4,324,019 + BWI76_RS20665 0.69 -0.1 4,324,114 - BWI76_RS20665 0.80 +0.6 4,324,152 + BWI76_RS20665 0.84 -0.2 4,324,189 + BWI76_RS20665 0.89 +0.1 4,324,220 + -0.9 4,324,515 + BWI76_RS20670 0.11 -2.6 4,324,533 + BWI76_RS20670 0.13 -3.7 4,325,054 - BWI76_RS20670 0.53 -3.0 4,325,140 + BWI76_RS20670 0.59 -3.9 4,325,549 + -2.1 4,325,701 - -1.2 4,325,726 + -0.7 4,325,731 + +1.9 4,325,779 - BWI76_RS20675 0.14 +0.2 4,325,864 + BWI76_RS20675 0.26 -1.5 4,325,904 + BWI76_RS20675 0.31 -0.8 4,326,249 + BWI76_RS20675 0.80 -0.5 4,326,249 + +1.5 4,326,258 - BWI76_RS20675 0.81 -1.6 4,326,315 + BWI76_RS20675 0.89 +2.1
Or see this region's nucleotide sequence