Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17875

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17865 and BWI76_RS17870 are separated by 3 nucleotidesBWI76_RS17870 and BWI76_RS17875 are separated by 162 nucleotidesBWI76_RS17875 and BWI76_RS17880 are separated by 131 nucleotides BWI76_RS17865: BWI76_RS17865 - aminodeoxychorismate synthase subunit I, at 3,714,375 to 3,715,730 _RS17865 BWI76_RS17870: BWI76_RS17870 - coenzyme A pyrophosphatase, at 3,715,734 to 3,716,312 _RS17870 BWI76_RS17875: BWI76_RS17875 - L-serine ammonia-lyase, at 3,716,475 to 3,717,839 _RS17875 BWI76_RS17880: BWI76_RS17880 - c-di-GMP phosphodiesterase, at 3,717,971 to 3,719,536 _RS17880 Position (kb) 3716 3717 3718Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3715.940 kb on + strand, within BWI76_RS17870at 3715.964 kb on - strand, within BWI76_RS17870at 3716.079 kb on - strand, within BWI76_RS17870at 3716.091 kb on - strand, within BWI76_RS17870at 3716.219 kb on + strand, within BWI76_RS17870at 3716.236 kb on - strand, within BWI76_RS17870at 3716.320 kb on - strandat 3717.066 kb on + strand, within BWI76_RS17875at 3717.504 kb on + strand, within BWI76_RS17875at 3717.569 kb on - strand, within BWI76_RS17875at 3717.635 kb on + strand, within BWI76_RS17875at 3717.723 kb on + strandat 3717.799 kb on - strandat 3717.915 kb on - strandat 3718.082 kb on - strandat 3718.178 kb on + strand, within BWI76_RS17880at 3718.401 kb on + strand, within BWI76_RS17880at 3718.667 kb on - strand, within BWI76_RS17880

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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3,715,940 + BWI76_RS17870 0.36 -0.7
3,715,964 - BWI76_RS17870 0.40 +0.8
3,716,079 - BWI76_RS17870 0.60 +0.7
3,716,091 - BWI76_RS17870 0.62 -0.3
3,716,219 + BWI76_RS17870 0.84 -0.1
3,716,236 - BWI76_RS17870 0.87 -0.6
3,716,320 - +3.5
3,717,066 + BWI76_RS17875 0.43 -2.6
3,717,504 + BWI76_RS17875 0.75 -0.9
3,717,569 - BWI76_RS17875 0.80 -3.4
3,717,635 + BWI76_RS17875 0.85 -4.0
3,717,723 + -0.3
3,717,799 - -1.1
3,717,915 - +1.4
3,718,082 - +0.2
3,718,178 + BWI76_RS17880 0.13 -0.9
3,718,401 + BWI76_RS17880 0.27 +1.2
3,718,667 - BWI76_RS17880 0.44 -0.4

Or see this region's nucleotide sequence