Experiment: m.b. D-Cycloserine 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1230 and Dshi_1231 are separated by 126 nucleotides Dshi_1231 and Dshi_1232 are separated by 215 nucleotides Dshi_1232 and Dshi_1233 are separated by 133 nucleotides
Dshi_1230: Dshi_1230 - kynureninase (RefSeq), at 1,273,036 to 1,274,217
_1230
Dshi_1231: Dshi_1231 - beta-lactamase domain protein (RefSeq), at 1,274,344 to 1,275,300
_1231
Dshi_1232: Dshi_1232 - isoprenylcysteine carboxyl methyltransferase family protein (RefSeq), at 1,275,516 to 1,275,992
_1232
Dshi_1233: Dshi_1233 - NAD(P) transhydrogenase, alpha subunit (RefSeq), at 1,276,126 to 1,277,742
_1233
Position (kb)
1274
1275
1276 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1273.344 kb on + strand, within Dshi_1230 at 1273.357 kb on + strand, within Dshi_1230 at 1273.567 kb on - strand, within Dshi_1230 at 1273.610 kb on - strand, within Dshi_1230 at 1273.646 kb on - strand, within Dshi_1230 at 1273.710 kb on - strand, within Dshi_1230 at 1273.834 kb on + strand, within Dshi_1230 at 1273.864 kb on + strand, within Dshi_1230 at 1273.991 kb on + strand, within Dshi_1230 at 1274.122 kb on - strand at 1274.183 kb on + strand at 1274.183 kb on + strand at 1274.191 kb on - strand at 1274.226 kb on - strand at 1274.238 kb on - strand at 1274.362 kb on + strand at 1274.431 kb on + strand at 1274.432 kb on - strand at 1274.455 kb on - strand, within Dshi_1231 at 1274.564 kb on + strand, within Dshi_1231 at 1274.601 kb on + strand, within Dshi_1231 at 1274.617 kb on + strand, within Dshi_1231 at 1274.622 kb on + strand, within Dshi_1231 at 1274.622 kb on + strand, within Dshi_1231 at 1274.630 kb on - strand, within Dshi_1231 at 1274.725 kb on + strand, within Dshi_1231 at 1274.754 kb on - strand, within Dshi_1231 at 1274.819 kb on - strand, within Dshi_1231 at 1274.819 kb on - strand, within Dshi_1231 at 1274.832 kb on - strand, within Dshi_1231 at 1274.834 kb on + strand, within Dshi_1231 at 1274.846 kb on + strand, within Dshi_1231 at 1274.854 kb on - strand, within Dshi_1231 at 1274.917 kb on + strand, within Dshi_1231 at 1275.049 kb on - strand, within Dshi_1231 at 1275.085 kb on + strand, within Dshi_1231 at 1275.135 kb on - strand, within Dshi_1231 at 1275.181 kb on + strand, within Dshi_1231 at 1275.224 kb on + strand at 1275.229 kb on - strand at 1275.232 kb on - strand at 1275.319 kb on - strand at 1275.344 kb on - strand at 1275.414 kb on + strand at 1275.422 kb on - strand at 1275.599 kb on - strand, within Dshi_1232 at 1275.664 kb on + strand, within Dshi_1232 at 1275.664 kb on + strand, within Dshi_1232 at 1275.679 kb on + strand, within Dshi_1232 at 1275.821 kb on + strand, within Dshi_1232 at 1275.821 kb on + strand, within Dshi_1232 at 1275.844 kb on + strand, within Dshi_1232 at 1275.860 kb on - strand, within Dshi_1232 at 1275.879 kb on + strand at 1275.887 kb on - strand, within Dshi_1232 at 1275.948 kb on - strand at 1275.976 kb on + strand at 1276.009 kb on + strand at 1276.168 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.048 mg/ml remove 1,273,344 + Dshi_1230 0.26 -0.7 1,273,357 + Dshi_1230 0.27 +0.0 1,273,567 - Dshi_1230 0.45 +0.1 1,273,610 - Dshi_1230 0.49 -0.2 1,273,646 - Dshi_1230 0.52 +0.2 1,273,710 - Dshi_1230 0.57 -0.0 1,273,834 + Dshi_1230 0.68 +0.4 1,273,864 + Dshi_1230 0.70 +0.1 1,273,991 + Dshi_1230 0.81 +0.2 1,274,122 - -1.1 1,274,183 + -0.6 1,274,183 + +0.3 1,274,191 - -1.5 1,274,226 - +0.6 1,274,238 - -0.6 1,274,362 + -0.5 1,274,431 + -0.2 1,274,432 - +0.9 1,274,455 - Dshi_1231 0.12 +0.3 1,274,564 + Dshi_1231 0.23 +0.2 1,274,601 + Dshi_1231 0.27 +0.1 1,274,617 + Dshi_1231 0.29 +0.4 1,274,622 + Dshi_1231 0.29 -0.2 1,274,622 + Dshi_1231 0.29 -0.8 1,274,630 - Dshi_1231 0.30 -1.0 1,274,725 + Dshi_1231 0.40 -0.4 1,274,754 - Dshi_1231 0.43 +1.0 1,274,819 - Dshi_1231 0.50 +0.5 1,274,819 - Dshi_1231 0.50 -0.8 1,274,832 - Dshi_1231 0.51 +0.4 1,274,834 + Dshi_1231 0.51 +0.6 1,274,846 + Dshi_1231 0.52 +0.0 1,274,854 - Dshi_1231 0.53 -0.7 1,274,917 + Dshi_1231 0.60 -0.1 1,275,049 - Dshi_1231 0.74 +0.3 1,275,085 + Dshi_1231 0.77 +0.2 1,275,135 - Dshi_1231 0.83 -1.7 1,275,181 + Dshi_1231 0.87 +0.1 1,275,224 + -0.2 1,275,229 - -0.1 1,275,232 - +0.6 1,275,319 - -1.4 1,275,344 - +0.3 1,275,414 + +0.4 1,275,422 - +0.5 1,275,599 - Dshi_1232 0.17 -2.8 1,275,664 + Dshi_1232 0.31 +0.3 1,275,664 + Dshi_1232 0.31 -1.3 1,275,679 + Dshi_1232 0.34 +0.9 1,275,821 + Dshi_1232 0.64 +1.3 1,275,821 + Dshi_1232 0.64 -1.0 1,275,844 + Dshi_1232 0.69 -0.0 1,275,860 - Dshi_1232 0.72 -0.4 1,275,879 + +1.4 1,275,887 - Dshi_1232 0.78 +0.3 1,275,948 - -0.3 1,275,976 + +0.1 1,276,009 + +0.1 1,276,168 + +0.4
Or see this region's nucleotide sequence