Experiment: m.b. D-Cycloserine 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0090 and Dshi_0091 are separated by 7 nucleotides Dshi_0091 and Dshi_0092 are separated by 1 nucleotides Dshi_0092 and Dshi_0093 are separated by 291 nucleotides
Dshi_0090: Dshi_0090 - peptidase M23B (RefSeq), at 88,672 to 89,775
_0090
Dshi_0091: Dshi_0091 - carboxyl-terminal protease (RefSeq), at 89,783 to 91,120
_0091
Dshi_0092: Dshi_0092 - NUDIX hydrolase (RefSeq), at 91,122 to 91,604
_0092
Dshi_0093: Dshi_0093 - hypothetical protein (RefSeq), at 91,896 to 92,264
_0093
Position (kb) 89
90
91
92 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 88.833 kb on - strand, within Dshi_0090 at 88.859 kb on + strand, within Dshi_0090 at 88.872 kb on + strand, within Dshi_0090 at 88.916 kb on + strand, within Dshi_0090 at 88.924 kb on - strand, within Dshi_0090 at 89.036 kb on + strand, within Dshi_0090 at 89.257 kb on - strand, within Dshi_0090 at 89.369 kb on - strand, within Dshi_0090 at 89.376 kb on - strand, within Dshi_0090 at 89.376 kb on - strand, within Dshi_0090 at 89.526 kb on - strand, within Dshi_0090 at 89.526 kb on - strand, within Dshi_0090 at 89.583 kb on + strand, within Dshi_0090 at 89.586 kb on + strand, within Dshi_0090 at 89.605 kb on - strand, within Dshi_0090 at 89.643 kb on + strand, within Dshi_0090 at 89.643 kb on + strand, within Dshi_0090 at 89.651 kb on - strand, within Dshi_0090 at 89.708 kb on + strand at 89.726 kb on + strand at 89.754 kb on + strand at 89.772 kb on - strand at 89.901 kb on + strand at 89.901 kb on + strand at 89.909 kb on - strand at 89.930 kb on - strand, within Dshi_0091 at 89.999 kb on - strand, within Dshi_0091 at 90.230 kb on - strand, within Dshi_0091 at 90.244 kb on + strand, within Dshi_0091 at 90.293 kb on + strand, within Dshi_0091 at 90.341 kb on - strand, within Dshi_0091 at 90.412 kb on - strand, within Dshi_0091 at 90.607 kb on + strand, within Dshi_0091 at 90.615 kb on - strand, within Dshi_0091 at 90.768 kb on + strand, within Dshi_0091 at 90.901 kb on + strand, within Dshi_0091 at 90.958 kb on - strand, within Dshi_0091 at 91.084 kb on + strand at 91.091 kb on - strand at 91.124 kb on - strand at 91.188 kb on - strand, within Dshi_0092 at 91.191 kb on + strand, within Dshi_0092 at 91.263 kb on - strand, within Dshi_0092 at 91.331 kb on + strand, within Dshi_0092 at 91.350 kb on + strand, within Dshi_0092 at 91.397 kb on + strand, within Dshi_0092 at 91.399 kb on + strand, within Dshi_0092 at 91.405 kb on - strand, within Dshi_0092 at 91.415 kb on - strand, within Dshi_0092 at 91.433 kb on - strand, within Dshi_0092 at 91.538 kb on - strand, within Dshi_0092 at 91.644 kb on + strand at 91.652 kb on - strand at 91.687 kb on - strand at 91.743 kb on - strand at 91.743 kb on - strand at 91.755 kb on - strand at 91.767 kb on + strand at 91.863 kb on - strand at 91.883 kb on + strand at 91.883 kb on + strand at 91.891 kb on - strand at 91.894 kb on + strand at 91.894 kb on + strand at 91.899 kb on + strand at 91.902 kb on - strand at 91.902 kb on - strand at 91.955 kb on - strand, within Dshi_0093 at 91.967 kb on - strand, within Dshi_0093 at 92.009 kb on - strand, within Dshi_0093
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.048 mg/ml remove 88,833 - Dshi_0090 0.15 +0.4 88,859 + Dshi_0090 0.17 -1.2 88,872 + Dshi_0090 0.18 -0.4 88,916 + Dshi_0090 0.22 -0.1 88,924 - Dshi_0090 0.23 -0.0 89,036 + Dshi_0090 0.33 -1.0 89,257 - Dshi_0090 0.53 +0.1 89,369 - Dshi_0090 0.63 -0.0 89,376 - Dshi_0090 0.64 -1.1 89,376 - Dshi_0090 0.64 +0.6 89,526 - Dshi_0090 0.77 -0.1 89,526 - Dshi_0090 0.77 +0.4 89,583 + Dshi_0090 0.83 -0.1 89,586 + Dshi_0090 0.83 +1.7 89,605 - Dshi_0090 0.85 -0.2 89,643 + Dshi_0090 0.88 +0.1 89,643 + Dshi_0090 0.88 +1.3 89,651 - Dshi_0090 0.89 -0.6 89,708 + -2.8 89,726 + -1.2 89,754 + -1.8 89,772 - -1.4 89,901 + -1.6 89,901 + -1.9 89,909 - -1.0 89,930 - Dshi_0091 0.11 -0.4 89,999 - Dshi_0091 0.16 -0.0 90,230 - Dshi_0091 0.33 -3.9 90,244 + Dshi_0091 0.34 -2.0 90,293 + Dshi_0091 0.38 -2.0 90,341 - Dshi_0091 0.42 -2.3 90,412 - Dshi_0091 0.47 +0.6 90,607 + Dshi_0091 0.62 -0.0 90,615 - Dshi_0091 0.62 -1.8 90,768 + Dshi_0091 0.74 -2.4 90,901 + Dshi_0091 0.84 -1.8 90,958 - Dshi_0091 0.88 -3.0 91,084 + -0.1 91,091 - -0.8 91,124 - +0.1 91,188 - Dshi_0092 0.14 -0.1 91,191 + Dshi_0092 0.14 -0.2 91,263 - Dshi_0092 0.29 -0.0 91,331 + Dshi_0092 0.43 -0.3 91,350 + Dshi_0092 0.47 +0.3 91,397 + Dshi_0092 0.57 -0.0 91,399 + Dshi_0092 0.57 +1.0 91,405 - Dshi_0092 0.59 +0.4 91,415 - Dshi_0092 0.61 -0.3 91,433 - Dshi_0092 0.64 +0.2 91,538 - Dshi_0092 0.86 +0.0 91,644 + -0.0 91,652 - -0.0 91,687 - +0.2 91,743 - -2.1 91,743 - +0.8 91,755 - +0.1 91,767 + -1.0 91,863 - +0.9 91,883 + -0.1 91,883 + +0.3 91,891 - +1.0 91,894 + -0.5 91,894 + +1.1 91,899 + -0.5 91,902 - +0.4 91,902 - +0.2 91,955 - Dshi_0093 0.16 -0.6 91,967 - Dshi_0093 0.19 -0.5 92,009 - Dshi_0093 0.31 +1.0
Or see this region's nucleotide sequence