Experiment: m.b. nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_622 and HP15_623 are separated by 15 nucleotides HP15_623 and HP15_624 are separated by 122 nucleotides HP15_624 and HP15_625 overlap by 9 nucleotides HP15_625 and HP15_626 are separated by 25 nucleotides
HP15_622: HP15_622 - exoribonuclease R, at 646,163 to 648,604
_622
HP15_623: HP15_623 - RNA methyltransferase, TrmH family, group 3, at 648,620 to 649,357
_623
HP15_624: HP15_624 - hypothetical protein, at 649,480 to 649,635
_624
HP15_625: HP15_625 - ribosomal protein S6, at 649,627 to 650,019
_625
HP15_626: HP15_626 - ribosomal protein S18, at 650,045 to 650,275
_626
Position (kb) 648
649
650 Strain fitness (log2 ratio) -1
0
1
2 at 647.624 kb on + strand, within HP15_622 at 647.624 kb on - strand, within HP15_622 at 647.624 kb on - strand, within HP15_622 at 647.626 kb on + strand, within HP15_622 at 647.632 kb on - strand, within HP15_622 at 647.680 kb on + strand, within HP15_622 at 647.697 kb on - strand, within HP15_622 at 647.752 kb on - strand, within HP15_622 at 648.022 kb on - strand, within HP15_622 at 648.336 kb on - strand, within HP15_622 at 648.382 kb on - strand at 648.694 kb on + strand, within HP15_623 at 648.727 kb on + strand, within HP15_623 at 648.958 kb on - strand, within HP15_623 at 649.014 kb on - strand, within HP15_623 at 649.291 kb on - strand at 649.301 kb on - strand at 649.358 kb on + strand at 650.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 10 mM remove 647,624 + HP15_622 0.60 -1.0 647,624 - HP15_622 0.60 +1.9 647,624 - HP15_622 0.60 +0.7 647,626 + HP15_622 0.60 +0.1 647,632 - HP15_622 0.60 +0.2 647,680 + HP15_622 0.62 +0.4 647,697 - HP15_622 0.63 +1.0 647,752 - HP15_622 0.65 -0.5 648,022 - HP15_622 0.76 +1.1 648,336 - HP15_622 0.89 +0.8 648,382 - -0.5 648,694 + HP15_623 0.10 -0.9 648,727 + HP15_623 0.14 -0.1 648,958 - HP15_623 0.46 -0.6 649,014 - HP15_623 0.53 -0.1 649,291 - -0.1 649,301 - -0.1 649,358 + -0.9 650,345 - +1.5
Or see this region's nucleotide sequence