Experiment: m.b. Phosphomycin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11590 and PGA1_c11600 overlap by 4 nucleotides PGA1_c11600 and PGA1_c11610 overlap by 4 nucleotides PGA1_c11610 and PGA1_c11620 are separated by 43 nucleotides PGA1_c11620 and PGA1_c11630 are separated by 265 nucleotides
PGA1_c11590: PGA1_c11590 - hypothetical protein, at 1,198,468 to 1,199,253
_c11590
PGA1_c11600: PGA1_c11600 - Uncharacterized protein conserved in bacteria, at 1,199,250 to 1,199,690
_c11600
PGA1_c11610: PGA1_c11610 - Uncharacterized protein conserved in bacteria, at 1,199,687 to 1,200,238
_c11610
PGA1_c11620: PGA1_c11620 - Uncharacterized conserved protein, at 1,200,282 to 1,200,575
_c11620
PGA1_c11630: PGA1_c11630 - valyl-tRNA synthetase ValS, at 1,200,841 to 1,203,915
_c11630
Position (kb)
1199
1200
1201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1198.700 kb on + strand, within PGA1_c11590 at 1198.723 kb on + strand, within PGA1_c11590 at 1198.757 kb on + strand, within PGA1_c11590 at 1198.758 kb on + strand, within PGA1_c11590 at 1198.766 kb on - strand, within PGA1_c11590 at 1198.877 kb on - strand, within PGA1_c11590 at 1198.981 kb on + strand, within PGA1_c11590 at 1199.024 kb on + strand, within PGA1_c11590 at 1199.033 kb on + strand, within PGA1_c11590 at 1199.046 kb on - strand, within PGA1_c11590 at 1199.060 kb on + strand, within PGA1_c11590 at 1199.068 kb on - strand, within PGA1_c11590 at 1199.070 kb on + strand, within PGA1_c11590 at 1199.070 kb on + strand, within PGA1_c11590 at 1199.077 kb on + strand, within PGA1_c11590 at 1199.080 kb on - strand, within PGA1_c11590 at 1199.112 kb on - strand, within PGA1_c11590 at 1199.194 kb on - strand at 1199.454 kb on + strand, within PGA1_c11600 at 1199.582 kb on - strand, within PGA1_c11600 at 1199.658 kb on + strand at 1199.713 kb on + strand at 1199.713 kb on + strand at 1199.713 kb on + strand at 1199.721 kb on - strand at 1199.782 kb on + strand, within PGA1_c11610 at 1199.827 kb on - strand, within PGA1_c11610 at 1199.841 kb on + strand, within PGA1_c11610 at 1199.841 kb on + strand, within PGA1_c11610 at 1199.841 kb on + strand, within PGA1_c11610 at 1199.849 kb on - strand, within PGA1_c11610 at 1199.860 kb on - strand, within PGA1_c11610 at 1199.890 kb on + strand, within PGA1_c11610 at 1200.036 kb on + strand, within PGA1_c11610 at 1200.036 kb on + strand, within PGA1_c11610 at 1200.044 kb on - strand, within PGA1_c11610 at 1200.100 kb on + strand, within PGA1_c11610 at 1200.112 kb on - strand, within PGA1_c11610 at 1200.235 kb on + strand at 1200.245 kb on + strand at 1200.245 kb on + strand at 1200.245 kb on + strand at 1200.245 kb on + strand at 1200.248 kb on - strand at 1200.253 kb on - strand at 1200.253 kb on - strand at 1200.253 kb on - strand at 1200.275 kb on - strand at 1200.281 kb on - strand at 1200.281 kb on - strand at 1200.327 kb on - strand, within PGA1_c11620 at 1200.340 kb on + strand, within PGA1_c11620 at 1200.340 kb on + strand, within PGA1_c11620 at 1200.348 kb on - strand, within PGA1_c11620 at 1200.348 kb on - strand, within PGA1_c11620 at 1200.434 kb on - strand, within PGA1_c11620 at 1200.446 kb on + strand, within PGA1_c11620 at 1200.504 kb on + strand, within PGA1_c11620 at 1200.511 kb on + strand, within PGA1_c11620 at 1200.549 kb on - strand at 1200.550 kb on - strand at 1200.583 kb on + strand at 1200.616 kb on + strand at 1200.616 kb on + strand at 1200.616 kb on + strand at 1200.616 kb on + strand at 1200.616 kb on + strand at 1200.616 kb on + strand at 1200.621 kb on + strand at 1200.624 kb on - strand at 1200.624 kb on - strand at 1200.624 kb on - strand at 1200.624 kb on - strand at 1200.640 kb on - strand at 1200.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 2 mg/ml remove 1,198,700 + PGA1_c11590 0.30 +0.3 1,198,723 + PGA1_c11590 0.32 -0.8 1,198,757 + PGA1_c11590 0.37 -0.4 1,198,758 + PGA1_c11590 0.37 -0.1 1,198,766 - PGA1_c11590 0.38 -0.2 1,198,877 - PGA1_c11590 0.52 -0.3 1,198,981 + PGA1_c11590 0.65 +0.1 1,199,024 + PGA1_c11590 0.71 -0.3 1,199,033 + PGA1_c11590 0.72 +0.2 1,199,046 - PGA1_c11590 0.74 +0.2 1,199,060 + PGA1_c11590 0.75 +0.5 1,199,068 - PGA1_c11590 0.76 +1.8 1,199,070 + PGA1_c11590 0.77 +0.4 1,199,070 + PGA1_c11590 0.77 -0.1 1,199,077 + PGA1_c11590 0.77 +0.5 1,199,080 - PGA1_c11590 0.78 -0.7 1,199,112 - PGA1_c11590 0.82 +2.0 1,199,194 - +0.6 1,199,454 + PGA1_c11600 0.46 +2.4 1,199,582 - PGA1_c11600 0.75 -0.7 1,199,658 + -0.9 1,199,713 + -0.4 1,199,713 + +0.1 1,199,713 + -0.0 1,199,721 - -0.4 1,199,782 + PGA1_c11610 0.17 -1.7 1,199,827 - PGA1_c11610 0.25 -0.0 1,199,841 + PGA1_c11610 0.28 -0.2 1,199,841 + PGA1_c11610 0.28 -1.5 1,199,841 + PGA1_c11610 0.28 -0.1 1,199,849 - PGA1_c11610 0.29 -0.6 1,199,860 - PGA1_c11610 0.31 -2.2 1,199,890 + PGA1_c11610 0.37 -0.6 1,200,036 + PGA1_c11610 0.63 -0.6 1,200,036 + PGA1_c11610 0.63 -0.4 1,200,044 - PGA1_c11610 0.65 -1.5 1,200,100 + PGA1_c11610 0.75 +1.4 1,200,112 - PGA1_c11610 0.77 -0.4 1,200,235 + +0.8 1,200,245 + -1.3 1,200,245 + +0.2 1,200,245 + -0.8 1,200,245 + -1.4 1,200,248 - -0.4 1,200,253 - -0.7 1,200,253 - +0.3 1,200,253 - +0.9 1,200,275 - -0.3 1,200,281 - +0.1 1,200,281 - -0.6 1,200,327 - PGA1_c11620 0.15 -1.6 1,200,340 + PGA1_c11620 0.20 +0.2 1,200,340 + PGA1_c11620 0.20 -0.2 1,200,348 - PGA1_c11620 0.22 -1.7 1,200,348 - PGA1_c11620 0.22 -0.2 1,200,434 - PGA1_c11620 0.52 -1.5 1,200,446 + PGA1_c11620 0.56 -1.7 1,200,504 + PGA1_c11620 0.76 -1.0 1,200,511 + PGA1_c11620 0.78 +0.3 1,200,549 - -0.1 1,200,550 - -1.4 1,200,583 + -1.0 1,200,616 + -0.7 1,200,616 + -1.4 1,200,616 + +0.3 1,200,616 + -0.0 1,200,616 + -0.4 1,200,616 + -0.3 1,200,621 + +1.9 1,200,624 - -0.8 1,200,624 - -0.7 1,200,624 - +0.5 1,200,624 - +1.0 1,200,640 - -0.4 1,200,712 - -0.0
Or see this region's nucleotide sequence