Experiment: PYE 0.5x 30C survival rep1 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00323 and CCNA_00324 are separated by 203 nucleotides CCNA_00324 and CCNA_00326 are separated by 243 nucleotides CCNA_00326 and CCNA_00327 are separated by 162 nucleotides CCNA_00327 and CCNA_00328 are separated by 84 nucleotides
CCNA_00323: CCNA_00323 - low-affinity zinc transport protein, at 336,631 to 337,728
_00323
CCNA_00324: CCNA_00324 - ExbD-family protein, at 337,932 to 338,390
_00324
CCNA_00326: CCNA_00326 - vegetatible incompatibility protein HET-E-1, at 338,634 to 339,665
_00326
CCNA_00327: CCNA_00327 - FMN-binding nitroreductase family enzyme, at 339,828 to 340,490
_00327
CCNA_00328: CCNA_00328 - monofunctional biosynthetic peptidoglycan transglycosylase MtgA, at 340,575 to 341,264
_00328
Position (kb) 338
339
340 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 337.644 kb on - strand at 337.663 kb on + strand at 337.671 kb on - strand at 337.688 kb on + strand at 337.731 kb on + strand at 337.732 kb on + strand at 337.747 kb on - strand at 337.759 kb on + strand at 337.779 kb on - strand at 337.795 kb on + strand at 337.796 kb on + strand at 337.803 kb on + strand at 337.803 kb on + strand at 337.811 kb on - strand at 337.816 kb on - strand at 337.830 kb on + strand at 337.837 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.842 kb on + strand at 337.850 kb on - strand at 337.850 kb on - strand at 337.850 kb on - strand at 337.891 kb on - strand at 337.961 kb on + strand at 337.963 kb on - strand at 337.963 kb on - strand at 337.999 kb on + strand, within CCNA_00324 at 337.999 kb on + strand, within CCNA_00324 at 338.011 kb on + strand, within CCNA_00324 at 338.228 kb on + strand, within CCNA_00324 at 338.244 kb on + strand, within CCNA_00324 at 338.252 kb on - strand, within CCNA_00324 at 338.261 kb on - strand, within CCNA_00324 at 338.309 kb on + strand, within CCNA_00324 at 338.382 kb on + strand at 338.385 kb on - strand at 338.385 kb on - strand at 338.390 kb on - strand at 338.390 kb on - strand at 338.400 kb on - strand at 338.452 kb on + strand at 338.455 kb on - strand at 338.460 kb on - strand at 338.603 kb on - strand at 338.604 kb on - strand at 338.604 kb on - strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.618 kb on + strand at 338.626 kb on - strand at 338.626 kb on - strand at 338.626 kb on - strand at 338.731 kb on - strand at 338.741 kb on - strand, within CCNA_00326 at 338.759 kb on - strand, within CCNA_00326 at 338.784 kb on + strand, within CCNA_00326 at 338.792 kb on - strand, within CCNA_00326 at 338.804 kb on + strand, within CCNA_00326 at 338.850 kb on + strand, within CCNA_00326 at 338.881 kb on + strand, within CCNA_00326 at 338.941 kb on + strand, within CCNA_00326 at 338.946 kb on + strand, within CCNA_00326 at 338.997 kb on + strand, within CCNA_00326 at 339.005 kb on - strand, within CCNA_00326 at 339.016 kb on + strand, within CCNA_00326 at 339.042 kb on + strand, within CCNA_00326 at 339.046 kb on - strand, within CCNA_00326 at 339.050 kb on - strand, within CCNA_00326 at 339.096 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.138 kb on + strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.146 kb on - strand, within CCNA_00326 at 339.191 kb on + strand, within CCNA_00326 at 339.289 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.308 kb on + strand, within CCNA_00326 at 339.317 kb on - strand, within CCNA_00326 at 339.327 kb on + strand, within CCNA_00326 at 339.347 kb on + strand, within CCNA_00326 at 339.347 kb on + strand, within CCNA_00326 at 339.355 kb on - strand, within CCNA_00326 at 339.415 kb on + strand, within CCNA_00326 at 339.423 kb on - strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.468 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.485 kb on + strand, within CCNA_00326 at 339.488 kb on - strand, within CCNA_00326 at 339.493 kb on - strand, within CCNA_00326 at 339.493 kb on - strand, within CCNA_00326 at 339.501 kb on - strand, within CCNA_00326 at 339.524 kb on + strand, within CCNA_00326 at 339.531 kb on + strand, within CCNA_00326 at 339.532 kb on - strand, within CCNA_00326 at 339.532 kb on - strand, within CCNA_00326 at 339.539 kb on - strand, within CCNA_00326 at 339.590 kb on - strand at 339.626 kb on - strand at 339.629 kb on - strand at 339.660 kb on + strand at 339.661 kb on - strand at 339.681 kb on + strand at 339.691 kb on + strand at 339.699 kb on - strand at 339.735 kb on + strand at 339.769 kb on + strand at 339.769 kb on + strand at 339.791 kb on + strand at 339.806 kb on + strand at 339.806 kb on + strand at 339.806 kb on + strand at 339.814 kb on - strand at 339.816 kb on + strand at 339.875 kb on + strand at 339.915 kb on - strand, within CCNA_00327 at 339.925 kb on + strand, within CCNA_00327 at 339.941 kb on - strand, within CCNA_00327 at 339.953 kb on + strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.982 kb on - strand, within CCNA_00327 at 339.997 kb on - strand, within CCNA_00327 at 340.006 kb on - strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.008 kb on + strand, within CCNA_00327 at 340.044 kb on + strand, within CCNA_00327 at 340.045 kb on - strand, within CCNA_00327 at 340.053 kb on - strand, within CCNA_00327 at 340.053 kb on - strand, within CCNA_00327 at 340.066 kb on - strand, within CCNA_00327 at 340.074 kb on - strand, within CCNA_00327 at 340.079 kb on - strand, within CCNA_00327 at 340.131 kb on + strand, within CCNA_00327 at 340.169 kb on + strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.177 kb on - strand, within CCNA_00327 at 340.194 kb on - strand, within CCNA_00327 at 340.198 kb on + strand, within CCNA_00327 at 340.200 kb on + strand, within CCNA_00327 at 340.200 kb on + strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.206 kb on - strand, within CCNA_00327 at 340.225 kb on - strand, within CCNA_00327 at 340.243 kb on + strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.251 kb on - strand, within CCNA_00327 at 340.272 kb on - strand, within CCNA_00327 at 340.274 kb on + strand, within CCNA_00327 at 340.280 kb on + strand, within CCNA_00327 at 340.288 kb on - strand, within CCNA_00327 at 340.291 kb on + strand, within CCNA_00327 at 340.292 kb on - strand, within CCNA_00327 at 340.338 kb on + strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.353 kb on - strand, within CCNA_00327 at 340.359 kb on + strand, within CCNA_00327 at 340.396 kb on + strand, within CCNA_00327 at 340.396 kb on + strand, within CCNA_00327 at 340.418 kb on + strand, within CCNA_00327 at 340.426 kb on - strand at 340.475 kb on - strand at 340.486 kb on + strand at 340.507 kb on - strand at 340.521 kb on - strand at 340.521 kb on - strand at 340.545 kb on + strand at 340.554 kb on - strand at 340.572 kb on + strand at 340.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 30C survival rep1 12 days remove 337,644 - +2.2 337,663 + +1.5 337,671 - -0.2 337,688 + +0.6 337,731 + +1.0 337,732 + +0.9 337,747 - -0.8 337,759 + +0.8 337,779 - +0.6 337,795 + +0.9 337,796 + +0.6 337,803 + +1.0 337,803 + +1.7 337,811 - -0.0 337,816 - +0.5 337,830 + +0.2 337,837 + +0.9 337,842 + +0.3 337,842 + +0.5 337,842 + +1.4 337,842 + +0.6 337,850 - +0.7 337,850 - +0.5 337,850 - +0.6 337,891 - +0.9 337,961 + -2.2 337,963 - -5.9 337,963 - -4.9 337,999 + CCNA_00324 0.15 +0.6 337,999 + CCNA_00324 0.15 -2.2 338,011 + CCNA_00324 0.17 +0.2 338,228 + CCNA_00324 0.64 +0.2 338,244 + CCNA_00324 0.68 -5.3 338,252 - CCNA_00324 0.70 -5.9 338,261 - CCNA_00324 0.72 -2.8 338,309 + CCNA_00324 0.82 -2.1 338,382 + -1.2 338,385 - -5.1 338,385 - -5.4 338,390 - -2.8 338,390 - -6.1 338,400 - +0.8 338,452 + -3.0 338,455 - -2.6 338,460 - -4.7 338,603 - -0.6 338,604 - -0.8 338,604 - -1.7 338,618 + -0.0 338,618 + +0.3 338,618 + -0.1 338,618 + -1.9 338,618 + +0.2 338,626 - +4.5 338,626 - +0.8 338,626 - -1.0 338,731 - -0.8 338,741 - CCNA_00326 0.10 +0.6 338,759 - CCNA_00326 0.12 -0.6 338,784 + CCNA_00326 0.15 +1.0 338,792 - CCNA_00326 0.15 -0.2 338,804 + CCNA_00326 0.16 +1.0 338,850 + CCNA_00326 0.21 +1.5 338,881 + CCNA_00326 0.24 +0.7 338,941 + CCNA_00326 0.30 +1.2 338,946 + CCNA_00326 0.30 -0.2 338,997 + CCNA_00326 0.35 +0.7 339,005 - CCNA_00326 0.36 -0.7 339,016 + CCNA_00326 0.37 +0.4 339,042 + CCNA_00326 0.40 +0.0 339,046 - CCNA_00326 0.40 -0.1 339,050 - CCNA_00326 0.40 -1.2 339,096 + CCNA_00326 0.45 +0.4 339,138 + CCNA_00326 0.49 +0.0 339,138 + CCNA_00326 0.49 +0.4 339,138 + CCNA_00326 0.49 +1.2 339,138 + CCNA_00326 0.49 -0.4 339,138 + CCNA_00326 0.49 +0.6 339,138 + CCNA_00326 0.49 -0.3 339,138 + CCNA_00326 0.49 +0.6 339,146 - CCNA_00326 0.50 +0.6 339,146 - CCNA_00326 0.50 +0.8 339,146 - CCNA_00326 0.50 -0.2 339,191 + CCNA_00326 0.54 +1.7 339,289 + CCNA_00326 0.63 +1.1 339,308 + CCNA_00326 0.65 +0.3 339,308 + CCNA_00326 0.65 +1.2 339,317 - CCNA_00326 0.66 -0.2 339,327 + CCNA_00326 0.67 +0.9 339,347 + CCNA_00326 0.69 +1.1 339,347 + CCNA_00326 0.69 +0.1 339,355 - CCNA_00326 0.70 -0.8 339,415 + CCNA_00326 0.76 +0.1 339,423 - CCNA_00326 0.76 -0.7 339,468 + CCNA_00326 0.81 +0.3 339,468 + CCNA_00326 0.81 +0.4 339,485 + CCNA_00326 0.82 +1.2 339,485 + CCNA_00326 0.82 +1.0 339,485 + CCNA_00326 0.82 +0.4 339,488 - CCNA_00326 0.83 -0.0 339,493 - CCNA_00326 0.83 -1.0 339,493 - CCNA_00326 0.83 -0.6 339,501 - CCNA_00326 0.84 -0.2 339,524 + CCNA_00326 0.86 -1.5 339,531 + CCNA_00326 0.87 +0.7 339,532 - CCNA_00326 0.87 +0.6 339,532 - CCNA_00326 0.87 +0.2 339,539 - CCNA_00326 0.88 -1.5 339,590 - +0.2 339,626 - -0.2 339,629 - -0.9 339,660 + -0.0 339,661 - -0.2 339,681 + +0.5 339,691 + +0.2 339,699 - +0.1 339,735 + +0.0 339,769 + -1.0 339,769 + -0.8 339,791 + -0.3 339,806 + +0.5 339,806 + -0.5 339,806 + -0.0 339,814 - +0.7 339,816 + -1.0 339,875 + +0.5 339,915 - CCNA_00327 0.13 +0.4 339,925 + CCNA_00327 0.15 -0.4 339,941 - CCNA_00327 0.17 +0.3 339,953 + CCNA_00327 0.19 -0.5 339,982 - CCNA_00327 0.23 -0.7 339,982 - CCNA_00327 0.23 -0.1 339,982 - CCNA_00327 0.23 -2.7 339,997 - CCNA_00327 0.25 +0.9 340,006 - CCNA_00327 0.27 -0.5 340,008 + CCNA_00327 0.27 -1.2 340,008 + CCNA_00327 0.27 -0.8 340,008 + CCNA_00327 0.27 +0.2 340,044 + CCNA_00327 0.33 -0.9 340,045 - CCNA_00327 0.33 -0.0 340,053 - CCNA_00327 0.34 +0.2 340,053 - CCNA_00327 0.34 -0.3 340,066 - CCNA_00327 0.36 -1.0 340,074 - CCNA_00327 0.37 +0.6 340,079 - CCNA_00327 0.38 -0.6 340,131 + CCNA_00327 0.46 +0.2 340,169 + CCNA_00327 0.51 -2.0 340,177 - CCNA_00327 0.53 +0.0 340,177 - CCNA_00327 0.53 +1.4 340,177 - CCNA_00327 0.53 -0.8 340,194 - CCNA_00327 0.55 -0.7 340,198 + CCNA_00327 0.56 +1.0 340,200 + CCNA_00327 0.56 -0.9 340,200 + CCNA_00327 0.56 -0.7 340,206 - CCNA_00327 0.57 +0.0 340,206 - CCNA_00327 0.57 +0.4 340,206 - CCNA_00327 0.57 -2.2 340,225 - CCNA_00327 0.60 -1.1 340,243 + CCNA_00327 0.63 -0.9 340,251 - CCNA_00327 0.64 -1.3 340,251 - CCNA_00327 0.64 +0.1 340,272 - CCNA_00327 0.67 +0.6 340,274 + CCNA_00327 0.67 -1.4 340,280 + CCNA_00327 0.68 -1.1 340,288 - CCNA_00327 0.69 +0.5 340,291 + CCNA_00327 0.70 +0.1 340,292 - CCNA_00327 0.70 -0.8 340,338 + CCNA_00327 0.77 +0.1 340,353 - CCNA_00327 0.79 -0.7 340,353 - CCNA_00327 0.79 +0.2 340,353 - CCNA_00327 0.79 -1.2 340,359 + CCNA_00327 0.80 -0.4 340,396 + CCNA_00327 0.86 -0.3 340,396 + CCNA_00327 0.86 -1.3 340,418 + CCNA_00327 0.89 -0.8 340,426 - -0.1 340,475 - +2.3 340,486 + +1.2 340,507 - -0.8 340,521 - -0.4 340,521 - -0.6 340,545 + -0.8 340,554 - +0.6 340,572 + -0.5 340,614 - -1.1
Or see this region's nucleotide sequence