Experiment: reconstituted MRS with Prebiotin-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13695 and BBR_RS13700 are separated by 130 nucleotides BBR_RS13700 and BBR_RS13705 are separated by 68 nucleotides BBR_RS13705 and BBR_RS13710 are separated by 126 nucleotides
BBR_RS13695: BBR_RS13695 - glutamate racemase, at 846,186 to 846,980
_RS13695
BBR_RS13700: BBR_RS13700 - diaminopimelate epimerase, at 847,111 to 848,010
_RS13700
BBR_RS13705: BBR_RS13705 - Conserved hypothetical membrane spanning protein, contains a vitamin K epoxide reductase domain, at 848,079 to 848,798
_RS13705
BBR_RS13710: BBR_RS13710 - PHP domain-containing protein, at 848,925 to 849,818
_RS13710
Position (kb)
847
848
849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 846.117 kb on + strand at 846.173 kb on - strand at 846.208 kb on - strand at 846.212 kb on + strand at 846.220 kb on - strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.051 kb on - strand at 847.058 kb on + strand at 847.077 kb on + strand at 847.098 kb on - strand at 847.098 kb on - strand at 847.169 kb on + strand at 847.216 kb on - strand, within BBR_RS13700 at 847.252 kb on + strand, within BBR_RS13700 at 847.266 kb on + strand, within BBR_RS13700 at 847.266 kb on + strand, within BBR_RS13700 at 847.269 kb on - strand, within BBR_RS13700 at 847.283 kb on + strand, within BBR_RS13700 at 847.288 kb on + strand, within BBR_RS13700 at 847.338 kb on - strand, within BBR_RS13700 at 847.360 kb on + strand, within BBR_RS13700 at 847.412 kb on - strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.447 kb on - strand, within BBR_RS13700 at 847.480 kb on + strand, within BBR_RS13700 at 847.489 kb on + strand, within BBR_RS13700 at 847.500 kb on + strand, within BBR_RS13700 at 847.507 kb on - strand, within BBR_RS13700 at 847.514 kb on + strand, within BBR_RS13700 at 847.514 kb on + strand, within BBR_RS13700 at 847.522 kb on - strand, within BBR_RS13700 at 847.522 kb on - strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.576 kb on - strand, within BBR_RS13700 at 847.576 kb on - strand, within BBR_RS13700 at 847.639 kb on - strand, within BBR_RS13700 at 847.688 kb on - strand, within BBR_RS13700 at 847.713 kb on + strand, within BBR_RS13700 at 847.726 kb on - strand, within BBR_RS13700 at 847.771 kb on + strand, within BBR_RS13700 at 847.783 kb on + strand, within BBR_RS13700 at 847.826 kb on + strand, within BBR_RS13700 at 847.834 kb on - strand, within BBR_RS13700 at 847.852 kb on - strand, within BBR_RS13700 at 847.854 kb on + strand, within BBR_RS13700 at 847.870 kb on + strand, within BBR_RS13700 at 847.878 kb on + strand, within BBR_RS13700 at 847.900 kb on - strand, within BBR_RS13700 at 847.925 kb on + strand at 847.952 kb on + strand at 847.953 kb on + strand at 847.974 kb on - strand at 847.993 kb on + strand at 848.003 kb on + strand at 848.006 kb on - strand at 848.020 kb on + strand at 848.101 kb on - strand at 848.103 kb on - strand at 848.145 kb on - strand at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.321 kb on - strand, within BBR_RS13705 at 848.350 kb on + strand, within BBR_RS13705 at 848.368 kb on - strand, within BBR_RS13705 at 848.387 kb on - strand, within BBR_RS13705 at 848.388 kb on + strand, within BBR_RS13705 at 848.391 kb on - strand, within BBR_RS13705 at 848.396 kb on - strand, within BBR_RS13705 at 848.481 kb on - strand, within BBR_RS13705 at 848.505 kb on - strand, within BBR_RS13705 at 848.610 kb on + strand, within BBR_RS13705 at 848.618 kb on - strand, within BBR_RS13705 at 848.694 kb on + strand, within BBR_RS13705 at 848.707 kb on - strand, within BBR_RS13705 at 848.721 kb on + strand, within BBR_RS13705 at 848.728 kb on - strand at 848.819 kb on - strand at 848.927 kb on + strand at 848.938 kb on - strand at 848.940 kb on + strand at 848.940 kb on - strand at 848.940 kb on - strand at 848.953 kb on + strand at 848.953 kb on + strand at 848.963 kb on + strand at 848.971 kb on - strand at 848.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Prebiotin-prebiotic 0.5 %w/v remove 846,117 + -0.7 846,173 - +0.6 846,208 - -1.6 846,212 + +0.0 846,220 - +2.0 847,043 + -1.1 847,043 + -2.4 847,043 + -0.6 847,043 + -0.6 847,043 + +0.3 847,043 + -0.2 847,043 + -0.1 847,051 - +0.5 847,058 + -0.4 847,077 + +0.8 847,098 - +0.6 847,098 - +1.0 847,169 + -0.8 847,216 - BBR_RS13700 0.12 -0.2 847,252 + BBR_RS13700 0.16 +0.3 847,266 + BBR_RS13700 0.17 +0.0 847,266 + BBR_RS13700 0.17 +0.3 847,269 - BBR_RS13700 0.18 +0.1 847,283 + BBR_RS13700 0.19 +0.4 847,288 + BBR_RS13700 0.20 -0.2 847,338 - BBR_RS13700 0.25 +0.5 847,360 + BBR_RS13700 0.28 -0.1 847,412 - BBR_RS13700 0.33 -0.3 847,419 + BBR_RS13700 0.34 +0.0 847,419 + BBR_RS13700 0.34 -0.5 847,419 + BBR_RS13700 0.34 -0.6 847,419 + BBR_RS13700 0.34 +0.2 847,447 - BBR_RS13700 0.37 -0.2 847,480 + BBR_RS13700 0.41 -0.2 847,489 + BBR_RS13700 0.42 -1.7 847,500 + BBR_RS13700 0.43 -0.1 847,507 - BBR_RS13700 0.44 -1.0 847,514 + BBR_RS13700 0.45 +0.3 847,514 + BBR_RS13700 0.45 +0.2 847,522 - BBR_RS13700 0.46 -1.0 847,522 - BBR_RS13700 0.46 +1.0 847,568 + BBR_RS13700 0.51 +0.7 847,568 + BBR_RS13700 0.51 +0.9 847,568 + BBR_RS13700 0.51 -0.4 847,576 - BBR_RS13700 0.52 -0.9 847,576 - BBR_RS13700 0.52 -0.3 847,639 - BBR_RS13700 0.59 -0.4 847,688 - BBR_RS13700 0.64 -0.3 847,713 + BBR_RS13700 0.67 -0.5 847,726 - BBR_RS13700 0.68 +0.6 847,771 + BBR_RS13700 0.73 +0.4 847,783 + BBR_RS13700 0.75 -0.0 847,826 + BBR_RS13700 0.79 +0.2 847,834 - BBR_RS13700 0.80 +0.3 847,852 - BBR_RS13700 0.82 -0.6 847,854 + BBR_RS13700 0.83 -0.6 847,870 + BBR_RS13700 0.84 +0.6 847,878 + BBR_RS13700 0.85 +0.6 847,900 - BBR_RS13700 0.88 +1.1 847,925 + +0.4 847,952 + +0.3 847,953 + +0.1 847,974 - +0.6 847,993 + +0.6 848,003 + +0.3 848,006 - -1.6 848,020 + +0.9 848,101 - +0.1 848,103 - -0.2 848,145 - +0.4 848,291 - BBR_RS13705 0.29 -1.3 848,291 - BBR_RS13705 0.29 -0.2 848,291 - BBR_RS13705 0.29 +1.2 848,305 + BBR_RS13705 0.31 +0.9 848,305 + BBR_RS13705 0.31 -0.4 848,321 - BBR_RS13705 0.34 -0.8 848,350 + BBR_RS13705 0.38 -0.2 848,368 - BBR_RS13705 0.40 +0.5 848,387 - BBR_RS13705 0.43 -1.5 848,388 + BBR_RS13705 0.43 +1.6 848,391 - BBR_RS13705 0.43 +0.8 848,396 - BBR_RS13705 0.44 -0.4 848,481 - BBR_RS13705 0.56 -1.0 848,505 - BBR_RS13705 0.59 -1.5 848,610 + BBR_RS13705 0.74 +0.1 848,618 - BBR_RS13705 0.75 -0.0 848,694 + BBR_RS13705 0.85 -0.4 848,707 - BBR_RS13705 0.87 +0.1 848,721 + BBR_RS13705 0.89 -0.5 848,728 - -2.1 848,819 - +0.1 848,927 + +0.6 848,938 - -0.6 848,940 + +0.9 848,940 - +0.4 848,940 - +0.2 848,953 + -0.7 848,953 + +0.8 848,963 + +1.2 848,971 - -0.3 848,971 - -0.7
Or see this region's nucleotide sequence