Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0482 and Dsui_0483 overlap by 4 nucleotides Dsui_0483 and Dsui_0484 are separated by 58 nucleotides Dsui_0484 and Dsui_0485 are separated by 74 nucleotides Dsui_0485 and Dsui_0486 overlap by 4 nucleotides
Dsui_0482: Dsui_0482 - TolQ protein, at 486,429 to 487,106
_0482
Dsui_0483: Dsui_0483 - tol-pal system-associated acyl-CoA thioesterase, at 487,103 to 487,528
_0483
Dsui_0484: Dsui_0484 - Holliday junction DNA helicase, RuvB subunit, at 487,587 to 488,669
_0484
Dsui_0485: Dsui_0485 - Holliday junction DNA helicase, RuvA subunit, at 488,744 to 489,328
_0485
Dsui_0486: Dsui_0486 - crossover junction endodeoxyribonuclease RuvC, at 489,325 to 489,930
_0486
Position (kb) 487
488
489 Strain fitness (log2 ratio) -2
-1
0
1 at 487.176 kb on + strand, within Dsui_0483 at 487.199 kb on - strand, within Dsui_0483 at 487.250 kb on - strand, within Dsui_0483 at 487.284 kb on + strand, within Dsui_0483 at 487.294 kb on - strand, within Dsui_0483 at 487.490 kb on + strand at 487.513 kb on - strand at 487.539 kb on - strand at 487.585 kb on + strand at 487.618 kb on - strand at 489.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 487,176 + Dsui_0483 0.17 +0.1 487,199 - Dsui_0483 0.23 +0.1 487,250 - Dsui_0483 0.35 +0.1 487,284 + Dsui_0483 0.42 +0.7 487,294 - Dsui_0483 0.45 -0.1 487,490 + -1.3 487,513 - -0.1 487,539 - -0.1 487,585 + -1.9 487,618 - -0.3 489,354 - -2.2
Or see this region's nucleotide sequence