Experiment: m.b. Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1198 and Dshi_1199 are separated by 152 nucleotides Dshi_1199 and Dshi_1200 are separated by 2 nucleotides Dshi_1200 and Dshi_1201 are separated by 88 nucleotides Dshi_1201 and Dshi_R0025 are separated by 51 nucleotides Dshi_R0025 and Dshi_1202 are separated by 18 nucleotides
Dshi_1198: Dshi_1198 - beta-lactamase (RefSeq), at 1,238,964 to 1,239,965
_1198
Dshi_1199: Dshi_1199 - Inositol-phosphate phosphatase (RefSeq), at 1,240,118 to 1,240,912
_1199
Dshi_1200: Dshi_1200 - Rhomboid family protein (RefSeq), at 1,240,915 to 1,241,691
_1200
Dshi_1201: Dshi_1201 - Tetratricopeptide TPR_2 repeat protein (RefSeq), at 1,241,780 to 1,242,331
_1201
Dshi_R0025: Dshi_R0025 - tRNA-Leu (RefSeq), at 1,242,383 to 1,242,468
_R0025
Dshi_1202: Dshi_1202 - heat shock protein DnaJ domain protein (RefSeq), at 1,242,487 to 1,243,116
_1202
Position (kb)
1240
1241
1242 Strain fitness (log2 ratio)
-1
0
1 at 1240.014 kb on - strand at 1240.025 kb on - strand at 1240.956 kb on + strand at 1241.158 kb on + strand, within Dshi_1200 at 1241.680 kb on + strand at 1241.691 kb on - strand at 1241.700 kb on + strand at 1241.705 kb on + strand at 1242.039 kb on + strand, within Dshi_1201 at 1242.098 kb on - strand, within Dshi_1201 at 1242.214 kb on - strand, within Dshi_1201 at 1242.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Lomefloxacin 0.001 mM remove 1,240,014 - +0.5 1,240,025 - -0.5 1,240,956 + -0.3 1,241,158 + Dshi_1200 0.31 -0.8 1,241,680 + -1.2 1,241,691 - +0.3 1,241,700 + -1.1 1,241,705 + -1.4 1,242,039 + Dshi_1201 0.47 -1.4 1,242,098 - Dshi_1201 0.58 -0.1 1,242,214 - Dshi_1201 0.79 +0.6 1,242,481 - -1.0
Or see this region's nucleotide sequence