Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4188 and BT4189 are separated by 6 nucleotides BT4189 and BT4190 are separated by 40 nucleotides BT4190 and BT4191 overlap by 35 nucleotides
BT4188: BT4188 - enoyl-[acyl-carrier-protein] reductase (NCBI ptt file), at 5,523,365 to 5,524,222
BT4188
BT4189: BT4189 - conserved hypothetical protein (NCBI ptt file), at 5,524,229 to 5,525,236
BT4189
BT4190: BT4190 - putative S-adenosylmethionine-dependent methytransferase (NCBI ptt file), at 5,525,277 to 5,525,981
BT4190
BT4191: BT4191 - hypothetical protein (NCBI ptt file), at 5,525,947 to 5,527,068
BT4191
Position (kb)
5524
5525
5526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5523.266 kb on + strand at 5523.266 kb on + strand at 5523.286 kb on + strand at 5523.287 kb on - strand at 5523.295 kb on + strand at 5523.330 kb on + strand at 5524.207 kb on + strand at 5524.208 kb on - strand at 5524.252 kb on + strand at 5524.252 kb on + strand at 5524.252 kb on + strand at 5524.258 kb on - strand at 5524.260 kb on + strand at 5524.261 kb on - strand at 5524.263 kb on + strand at 5524.263 kb on + strand at 5524.267 kb on + strand at 5524.268 kb on - strand at 5524.344 kb on - strand, within BT4189 at 5524.344 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.365 kb on - strand, within BT4189 at 5524.446 kb on + strand, within BT4189 at 5524.454 kb on - strand, within BT4189 at 5524.540 kb on - strand at 5524.540 kb on - strand, within BT4189 at 5524.540 kb on - strand, within BT4189 at 5524.630 kb on - strand, within BT4189 at 5524.684 kb on + strand, within BT4189 at 5524.684 kb on + strand, within BT4189 at 5524.685 kb on - strand, within BT4189 at 5524.712 kb on + strand, within BT4189 at 5524.712 kb on + strand, within BT4189 at 5524.714 kb on + strand, within BT4189 at 5524.715 kb on - strand, within BT4189 at 5524.751 kb on + strand, within BT4189 at 5524.751 kb on + strand, within BT4189 at 5524.850 kb on - strand, within BT4189 at 5524.850 kb on - strand, within BT4189 at 5524.997 kb on + strand, within BT4189 at 5524.998 kb on - strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5524.999 kb on + strand, within BT4189 at 5525.000 kb on - strand, within BT4189 at 5525.134 kb on - strand, within BT4189 at 5525.141 kb on + strand at 5525.155 kb on + strand at 5525.188 kb on + strand at 5525.188 kb on + strand at 5525.203 kb on + strand at 5525.204 kb on - strand at 5525.242 kb on - strand at 5525.246 kb on - strand at 5525.248 kb on + strand at 5525.248 kb on + strand at 5525.255 kb on + strand at 5525.259 kb on + strand at 5525.266 kb on + strand at 5525.293 kb on - strand at 5525.338 kb on + strand at 5525.338 kb on + strand at 5525.338 kb on + strand at 5525.346 kb on + strand at 5525.346 kb on + strand at 5525.348 kb on + strand, within BT4190 at 5525.361 kb on - strand, within BT4190 at 5525.478 kb on - strand, within BT4190 at 5525.478 kb on - strand, within BT4190 at 5525.493 kb on - strand, within BT4190 at 5525.493 kb on - strand, within BT4190 at 5525.502 kb on + strand at 5525.714 kb on - strand, within BT4190 at 5525.732 kb on + strand, within BT4190 at 5525.785 kb on + strand, within BT4190 at 5525.785 kb on + strand at 5525.813 kb on + strand, within BT4190 at 5525.822 kb on - strand, within BT4190 at 5525.822 kb on - strand, within BT4190 at 5525.828 kb on + strand, within BT4190 at 5525.861 kb on - strand, within BT4190 at 5525.864 kb on + strand, within BT4190 at 5525.864 kb on + strand, within BT4190 at 5525.865 kb on - strand, within BT4190 at 5525.865 kb on - strand, within BT4190 at 5525.876 kb on + strand, within BT4190 at 5525.900 kb on - strand, within BT4190 at 5525.934 kb on - strand at 5525.972 kb on - strand at 5525.974 kb on - strand at 5525.980 kb on - strand at 5525.999 kb on + strand at 5526.073 kb on - strand, within BT4191 at 5526.073 kb on - strand, within BT4191 at 5526.102 kb on + strand, within BT4191
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 5,523,266 + -3.5 5,523,266 + -0.3 5,523,286 + -0.8 5,523,287 - -0.6 5,523,295 + -1.3 5,523,330 + -1.3 5,524,207 + -3.2 5,524,208 - -1.2 5,524,252 + -1.6 5,524,252 + -0.0 5,524,252 + -0.5 5,524,258 - -2.5 5,524,260 + -0.6 5,524,261 - +0.7 5,524,263 + -2.0 5,524,263 + -1.3 5,524,267 + -0.0 5,524,268 - -1.3 5,524,344 - BT4189 0.11 -2.3 5,524,344 - BT4189 0.11 -0.0 5,524,365 - BT4189 0.13 -1.4 5,524,365 - BT4189 0.13 -1.2 5,524,365 - BT4189 0.13 -2.0 5,524,446 + BT4189 0.22 -0.6 5,524,454 - BT4189 0.22 -0.8 5,524,540 - -0.3 5,524,540 - BT4189 0.31 -2.3 5,524,540 - BT4189 0.31 -2.1 5,524,630 - BT4189 0.40 -1.6 5,524,684 + BT4189 0.45 -3.4 5,524,684 + BT4189 0.45 +1.0 5,524,685 - BT4189 0.45 -0.6 5,524,712 + BT4189 0.48 -3.6 5,524,712 + BT4189 0.48 -3.3 5,524,714 + BT4189 0.48 -1.7 5,524,715 - BT4189 0.48 +1.0 5,524,751 + BT4189 0.52 -1.2 5,524,751 + BT4189 0.52 -2.0 5,524,850 - BT4189 0.62 -2.5 5,524,850 - BT4189 0.62 -2.2 5,524,997 + BT4189 0.76 -1.3 5,524,998 - BT4189 0.76 -0.6 5,524,999 + BT4189 0.76 -1.6 5,524,999 + BT4189 0.76 +1.0 5,524,999 + BT4189 0.76 -2.2 5,525,000 - BT4189 0.76 +0.4 5,525,134 - BT4189 0.90 -0.6 5,525,141 + -0.0 5,525,155 + +0.4 5,525,188 + +0.4 5,525,188 + -1.1 5,525,203 + -3.8 5,525,204 - -1.0 5,525,242 - +0.0 5,525,246 - -2.0 5,525,248 + -1.8 5,525,248 + +1.0 5,525,255 + -0.9 5,525,259 + -1.4 5,525,266 + -0.1 5,525,293 - -1.0 5,525,338 + +1.0 5,525,338 + -1.0 5,525,338 + +0.3 5,525,346 + -1.3 5,525,346 + -1.8 5,525,348 + BT4190 0.10 +1.0 5,525,361 - BT4190 0.12 -2.9 5,525,478 - BT4190 0.29 -0.3 5,525,478 - BT4190 0.29 +0.1 5,525,493 - BT4190 0.31 -1.6 5,525,493 - BT4190 0.31 -0.8 5,525,502 + -1.0 5,525,714 - BT4190 0.62 -1.9 5,525,732 + BT4190 0.65 -3.5 5,525,785 + BT4190 0.72 +1.0 5,525,785 + +1.0 5,525,813 + BT4190 0.76 -1.1 5,525,822 - BT4190 0.77 -1.3 5,525,822 - BT4190 0.77 +0.0 5,525,828 + BT4190 0.78 +0.0 5,525,861 - BT4190 0.83 -1.5 5,525,864 + BT4190 0.83 +0.0 5,525,864 + BT4190 0.83 -1.6 5,525,865 - BT4190 0.83 -1.0 5,525,865 - BT4190 0.83 +0.1 5,525,876 + BT4190 0.85 +0.4 5,525,900 - BT4190 0.88 +0.4 5,525,934 - -1.1 5,525,972 - +0.6 5,525,974 - +0.0 5,525,980 - -3.2 5,525,999 + +0.0 5,526,073 - BT4191 0.11 -1.2 5,526,073 - BT4191 0.11 +0.2 5,526,102 + BT4191 0.14 -0.0
Or see this region's nucleotide sequence