Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1960 and BT1961 are separated by 84 nucleotides BT1961 and BT1962 are separated by 429 nucleotides BT1962 and BT1963 are separated by 229 nucleotides
BT1960: BT1960 - integrase (NCBI ptt file), at 2,461,168 to 2,461,806
BT1960
BT1961: BT1961 - transposase (NCBI ptt file), at 2,461,891 to 2,462,061
BT1961
BT1962: BT1962 - hypothetical protein (NCBI ptt file), at 2,462,491 to 2,463,267
BT1962
BT1963: BT1963 - putative transcriptional regulator (NCBI ptt file), at 2,463,497 to 2,464,225
BT1963
Position (kb)
2462
2463
2464 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2461.492 kb on - strand, within BT1960 at 2461.547 kb on + strand, within BT1960 at 2461.547 kb on + strand, within BT1960 at 2461.548 kb on - strand, within BT1960 at 2461.567 kb on - strand, within BT1960 at 2461.625 kb on + strand, within BT1960 at 2461.737 kb on - strand, within BT1960 at 2461.757 kb on - strand at 2461.757 kb on - strand at 2461.838 kb on + strand at 2461.889 kb on + strand at 2461.944 kb on + strand, within BT1961 at 2462.113 kb on + strand at 2462.114 kb on - strand at 2462.115 kb on + strand at 2462.115 kb on + strand at 2462.115 kb on + strand at 2462.115 kb on + strand at 2462.115 kb on + strand at 2462.115 kb on + strand at 2462.116 kb on - strand at 2462.116 kb on - strand at 2462.134 kb on + strand at 2462.178 kb on + strand at 2462.179 kb on - strand at 2462.201 kb on + strand at 2462.202 kb on - strand at 2462.202 kb on - strand at 2462.202 kb on - strand at 2462.203 kb on + strand at 2462.206 kb on + strand at 2462.207 kb on - strand at 2462.241 kb on + strand at 2462.241 kb on + strand at 2462.245 kb on + strand at 2462.263 kb on + strand at 2462.263 kb on + strand at 2462.264 kb on - strand at 2462.264 kb on - strand at 2462.363 kb on + strand at 2462.364 kb on - strand at 2462.364 kb on - strand at 2462.463 kb on + strand at 2462.505 kb on + strand at 2462.506 kb on - strand at 2462.506 kb on - strand at 2462.531 kb on - strand at 2462.531 kb on - strand at 2462.682 kb on + strand, within BT1962 at 2462.684 kb on + strand, within BT1962 at 2463.125 kb on - strand, within BT1962 at 2463.125 kb on - strand, within BT1962 at 2463.297 kb on + strand at 2463.324 kb on - strand at 2463.366 kb on + strand at 2463.366 kb on + strand at 2463.367 kb on - strand at 2463.370 kb on + strand at 2463.373 kb on + strand at 2463.373 kb on + strand at 2463.374 kb on - strand at 2463.387 kb on + strand at 2463.469 kb on + strand at 2463.477 kb on + strand at 2463.576 kb on + strand, within BT1963 at 2463.610 kb on + strand, within BT1963 at 2463.610 kb on + strand, within BT1963 at 2463.616 kb on + strand, within BT1963 at 2463.636 kb on + strand, within BT1963 at 2463.662 kb on + strand, within BT1963 at 2463.663 kb on - strand, within BT1963 at 2463.664 kb on + strand, within BT1963 at 2463.665 kb on - strand, within BT1963 at 2463.699 kb on - strand, within BT1963 at 2463.801 kb on - strand, within BT1963 at 2463.990 kb on - strand, within BT1963 at 2463.992 kb on + strand, within BT1963 at 2464.003 kb on - strand, within BT1963 at 2464.003 kb on - strand, within BT1963 at 2464.073 kb on - strand, within BT1963 at 2464.137 kb on + strand, within BT1963 at 2464.179 kb on + strand at 2464.179 kb on + strand at 2464.180 kb on - strand at 2464.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 2,461,492 - BT1960 0.51 +1.0 2,461,547 + BT1960 0.59 -2.0 2,461,547 + BT1960 0.59 -1.8 2,461,548 - BT1960 0.59 -0.7 2,461,567 - BT1960 0.62 -0.1 2,461,625 + BT1960 0.72 -1.3 2,461,737 - BT1960 0.89 -0.4 2,461,757 - +0.0 2,461,757 - +1.0 2,461,838 + -1.8 2,461,889 + +0.2 2,461,944 + BT1961 0.31 +1.0 2,462,113 + -0.6 2,462,114 - -0.1 2,462,115 + +0.5 2,462,115 + -5.0 2,462,115 + -2.6 2,462,115 + +0.0 2,462,115 + +0.2 2,462,115 + -3.3 2,462,116 - -1.3 2,462,116 - -1.0 2,462,134 + -2.8 2,462,178 + -2.8 2,462,179 - -1.3 2,462,201 + -3.6 2,462,202 - -3.0 2,462,202 - +0.0 2,462,202 - -0.6 2,462,203 + -3.1 2,462,206 + -1.1 2,462,207 - +0.0 2,462,241 + -1.2 2,462,241 + -1.9 2,462,245 + -2.0 2,462,263 + +0.0 2,462,263 + +0.0 2,462,264 - +0.3 2,462,264 - -2.9 2,462,363 + +1.0 2,462,364 - -0.1 2,462,364 - -0.6 2,462,463 + -0.3 2,462,505 + -2.7 2,462,506 - -1.3 2,462,506 - -2.4 2,462,531 - +0.6 2,462,531 - +0.3 2,462,682 + BT1962 0.25 -4.3 2,462,684 + BT1962 0.25 -2.2 2,463,125 - BT1962 0.82 +1.0 2,463,125 - BT1962 0.82 -2.7 2,463,297 + -1.8 2,463,324 - -2.0 2,463,366 + -0.8 2,463,366 + -2.6 2,463,367 - +1.0 2,463,370 + +0.4 2,463,373 + -1.0 2,463,373 + +0.4 2,463,374 - -1.5 2,463,387 + -1.3 2,463,469 + -3.3 2,463,477 + -2.4 2,463,576 + BT1963 0.11 +1.0 2,463,610 + BT1963 0.16 +0.0 2,463,610 + BT1963 0.16 +0.0 2,463,616 + BT1963 0.16 +0.0 2,463,636 + BT1963 0.19 -1.8 2,463,662 + BT1963 0.23 -0.6 2,463,663 - BT1963 0.23 +1.0 2,463,664 + BT1963 0.23 +0.0 2,463,665 - BT1963 0.23 -1.2 2,463,699 - BT1963 0.28 -1.6 2,463,801 - BT1963 0.42 -2.1 2,463,990 - BT1963 0.68 -1.6 2,463,992 + BT1963 0.68 -2.0 2,464,003 - BT1963 0.69 -1.0 2,464,003 - BT1963 0.69 -1.5 2,464,073 - BT1963 0.79 -0.3 2,464,137 + BT1963 0.88 -4.4 2,464,179 + -0.6 2,464,179 + -4.4 2,464,180 - -0.6 2,464,250 - +0.0
Or see this region's nucleotide sequence