Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1135 and BT1136 are separated by 9 nucleotides BT1136 and BT1137 are separated by 149 nucleotides BT1137 and BT1138 are separated by 41 nucleotides BT1138 and BT1139 are separated by 197 nucleotides
BT1135: BT1135 - hypothetical protein (NCBI ptt file), at 1,420,882 to 1,421,481
BT1135
BT1136: BT1136 - hypothetical protein (NCBI ptt file), at 1,421,491 to 1,421,703
BT1136
BT1137: BT1137 - hypothetical protein (NCBI ptt file), at 1,421,853 to 1,422,119
BT1137
BT1138: BT1138 - transposase (NCBI ptt file), at 1,422,161 to 1,423,396
BT1138
BT1139: BT1139 - conserved hypothetical protein (NCBI ptt file), at 1,423,594 to 1,424,652
BT1139
Position (kb)
1422
1423
1424 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1421.180 kb on + strand, within BT1135 at 1421.229 kb on + strand, within BT1135 at 1421.245 kb on + strand, within BT1135 at 1421.277 kb on + strand, within BT1135 at 1421.292 kb on + strand, within BT1135 at 1421.293 kb on - strand, within BT1135 at 1421.293 kb on - strand, within BT1135 at 1421.436 kb on + strand at 1421.436 kb on + strand at 1421.437 kb on - strand at 1421.439 kb on - strand at 1421.658 kb on - strand, within BT1136 at 1421.967 kb on + strand, within BT1137 at 1422.202 kb on + strand at 1422.202 kb on + strand at 1422.257 kb on + strand at 1422.413 kb on + strand, within BT1138 at 1422.449 kb on + strand, within BT1138 at 1422.450 kb on - strand, within BT1138 at 1422.486 kb on - strand, within BT1138 at 1422.759 kb on + strand, within BT1138 at 1422.975 kb on + strand, within BT1138 at 1422.975 kb on + strand, within BT1138 at 1422.975 kb on + strand, within BT1138 at 1422.976 kb on - strand, within BT1138 at 1422.976 kb on - strand, within BT1138 at 1422.996 kb on + strand, within BT1138 at 1422.996 kb on + strand, within BT1138 at 1422.997 kb on - strand, within BT1138 at 1423.434 kb on + strand at 1423.698 kb on + strand at 1423.761 kb on - strand, within BT1139 at 1423.775 kb on + strand, within BT1139 at 1423.799 kb on + strand, within BT1139 at 1423.799 kb on + strand, within BT1139 at 1423.808 kb on - strand, within BT1139 at 1423.808 kb on - strand, within BT1139 at 1423.888 kb on - strand, within BT1139 at 1423.944 kb on + strand, within BT1139 at 1423.969 kb on + strand, within BT1139 at 1424.009 kb on - strand, within BT1139 at 1424.074 kb on + strand, within BT1139 at 1424.102 kb on + strand, within BT1139 at 1424.135 kb on + strand, within BT1139 at 1424.387 kb on - strand, within BT1139
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 1,421,180 + BT1135 0.50 +0.1 1,421,229 + BT1135 0.58 -0.9 1,421,245 + BT1135 0.60 -1.5 1,421,277 + BT1135 0.66 -0.5 1,421,292 + BT1135 0.68 -1.1 1,421,293 - BT1135 0.69 -0.5 1,421,293 - BT1135 0.69 -0.9 1,421,436 + -0.2 1,421,436 + -1.7 1,421,437 - -1.7 1,421,439 - -0.3 1,421,658 - BT1136 0.78 +0.3 1,421,967 + BT1137 0.43 -4.4 1,422,202 + +1.1 1,422,202 + +0.1 1,422,257 + -1.5 1,422,413 + BT1138 0.20 +0.1 1,422,449 + BT1138 0.23 -2.6 1,422,450 - BT1138 0.23 -0.9 1,422,486 - BT1138 0.26 +1.1 1,422,759 + BT1138 0.48 +1.1 1,422,975 + BT1138 0.66 -2.2 1,422,975 + BT1138 0.66 +1.1 1,422,975 + BT1138 0.66 -1.5 1,422,976 - BT1138 0.66 -1.7 1,422,976 - BT1138 0.66 -2.3 1,422,996 + BT1138 0.68 -2.0 1,422,996 + BT1138 0.68 -2.3 1,422,997 - BT1138 0.68 -1.8 1,423,434 + +0.1 1,423,698 + -0.5 1,423,761 - BT1139 0.16 -1.5 1,423,775 + BT1139 0.17 -2.8 1,423,799 + BT1139 0.19 -1.9 1,423,799 + BT1139 0.19 -4.6 1,423,808 - BT1139 0.20 +1.7 1,423,808 - BT1139 0.20 -2.5 1,423,888 - BT1139 0.28 -2.8 1,423,944 + BT1139 0.33 -1.7 1,423,969 + BT1139 0.35 +0.1 1,424,009 - BT1139 0.39 -0.5 1,424,074 + BT1139 0.45 -2.0 1,424,102 + BT1139 0.48 +1.1 1,424,135 + BT1139 0.51 -1.4 1,424,387 - BT1139 0.75 -1.1
Or see this region's nucleotide sequence