Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0578 and BT0579 are separated by 42 nucleotides BT0579 and BT0580 are separated by 75 nucleotides
BT0578: BT0578 - excinuclease ABC subunit A (NCBI ptt file), at 714,364 to 717,204
BT0578
BT0579: BT0579 - putative transcription regulator (NCBI ptt file), at 717,247 to 717,726
BT0579
BT0580: BT0580 - putative H+/peptide symporter (NCBI ptt file), at 717,802 to 719,316
BT0580
Position (kb) 717
718 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 716.256 kb on - strand, within BT0578 at 716.328 kb on + strand, within BT0578 at 716.329 kb on - strand, within BT0578 at 716.420 kb on + strand, within BT0578 at 716.425 kb on - strand, within BT0578 at 716.488 kb on - strand, within BT0578 at 716.804 kb on + strand, within BT0578 at 716.835 kb on + strand, within BT0578 at 716.838 kb on + strand, within BT0578 at 716.894 kb on + strand, within BT0578 at 716.985 kb on - strand at 717.000 kb on + strand at 717.001 kb on - strand at 717.001 kb on - strand at 717.003 kb on + strand at 717.046 kb on - strand at 717.074 kb on - strand at 717.081 kb on - strand at 717.116 kb on - strand at 717.127 kb on + strand at 717.169 kb on - strand at 717.206 kb on - strand at 717.216 kb on - strand at 717.228 kb on - strand at 717.230 kb on + strand at 717.390 kb on + strand, within BT0579 at 717.756 kb on - strand at 717.801 kb on + strand at 717.830 kb on + strand at 717.872 kb on - strand at 717.910 kb on - strand at 717.910 kb on - strand at 717.943 kb on - strand at 717.946 kb on + strand at 717.947 kb on - strand at 717.993 kb on + strand, within BT0580 at 717.994 kb on - strand, within BT0580 at 718.019 kb on - strand, within BT0580 at 718.029 kb on + strand, within BT0580 at 718.077 kb on + strand, within BT0580 at 718.105 kb on - strand, within BT0580 at 718.125 kb on + strand, within BT0580 at 718.125 kb on + strand, within BT0580 at 718.204 kb on + strand, within BT0580 at 718.220 kb on - strand, within BT0580 at 718.256 kb on - strand, within BT0580 at 718.257 kb on + strand, within BT0580 at 718.257 kb on + strand, within BT0580 at 718.307 kb on + strand, within BT0580 at 718.308 kb on - strand, within BT0580 at 718.308 kb on - strand, within BT0580 at 718.311 kb on + strand, within BT0580 at 718.312 kb on - strand, within BT0580 at 718.386 kb on + strand, within BT0580 at 718.405 kb on - strand, within BT0580 at 718.498 kb on - strand, within BT0580 at 718.524 kb on - strand, within BT0580 at 718.528 kb on - strand, within BT0580 at 718.632 kb on - strand, within BT0580 at 718.662 kb on + strand, within BT0580 at 718.662 kb on + strand, within BT0580 at 718.667 kb on - strand, within BT0580 at 718.667 kb on - strand, within BT0580 at 718.667 kb on - strand, within BT0580 at 718.667 kb on - strand, within BT0580
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 716,256 - BT0578 0.67 -2.5 716,328 + BT0578 0.69 -2.8 716,329 - BT0578 0.69 -1.3 716,420 + BT0578 0.72 -3.5 716,425 - BT0578 0.73 -0.9 716,488 - BT0578 0.75 -2.7 716,804 + BT0578 0.86 -4.1 716,835 + BT0578 0.87 -0.9 716,838 + BT0578 0.87 -1.3 716,894 + BT0578 0.89 -1.3 716,985 - +0.1 717,000 + -0.5 717,001 - -3.6 717,001 - -3.3 717,003 + -0.5 717,046 - -0.5 717,074 - -1.9 717,081 - -3.3 717,116 - -1.3 717,127 + -0.5 717,169 - -1.7 717,206 - +0.7 717,216 - -1.3 717,228 - +1.1 717,230 + +0.1 717,390 + BT0579 0.30 -2.1 717,756 - +1.1 717,801 + -0.8 717,830 + -0.9 717,872 - -0.5 717,910 - -2.0 717,910 - -0.5 717,943 - +0.5 717,946 + -2.5 717,947 - +0.1 717,993 + BT0580 0.13 +0.1 717,994 - BT0580 0.13 -0.9 718,019 - BT0580 0.14 +1.1 718,029 + BT0580 0.15 -1.3 718,077 + BT0580 0.18 -0.5 718,105 - BT0580 0.20 -3.0 718,125 + BT0580 0.21 -0.5 718,125 + BT0580 0.21 -2.1 718,204 + BT0580 0.27 -0.5 718,220 - BT0580 0.28 +0.1 718,256 - BT0580 0.30 -1.2 718,257 + BT0580 0.30 +0.1 718,257 + BT0580 0.30 -0.2 718,307 + BT0580 0.33 -1.5 718,308 - BT0580 0.33 -1.5 718,308 - BT0580 0.33 -1.3 718,311 + BT0580 0.34 +1.1 718,312 - BT0580 0.34 -0.6 718,386 + BT0580 0.39 -2.9 718,405 - BT0580 0.40 -2.4 718,498 - BT0580 0.46 -2.3 718,524 - BT0580 0.48 -3.2 718,528 - BT0580 0.48 -0.5 718,632 - BT0580 0.55 -0.5 718,662 + BT0580 0.57 -1.5 718,662 + BT0580 0.57 +0.1 718,667 - BT0580 0.57 -2.5 718,667 - BT0580 0.57 -0.9 718,667 - BT0580 0.57 -1.4 718,667 - BT0580 0.57 -1.3
Or see this region's nucleotide sequence