Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0575 and BT0576 are separated by 127 nucleotides BT0576 and BT0577 are separated by 7 nucleotides BT0577 and BT0578 are separated by 142 nucleotides
BT0575: BT0575 - conserved hypothetical protein (NCBI ptt file), at 711,154 to 711,597
BT0575
BT0576: BT0576 - hypothetical protein (NCBI ptt file), at 711,725 to 712,447
BT0576
BT0577: BT0577 - LysM-repeat proteins and domains (NCBI ptt file), at 712,455 to 714,221
BT0577
BT0578: BT0578 - excinuclease ABC subunit A (NCBI ptt file), at 714,364 to 717,204
BT0578
Position (kb) 712
713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 711.632 kb on + strand at 711.632 kb on + strand at 711.637 kb on + strand at 711.637 kb on + strand at 711.763 kb on + strand at 712.047 kb on - strand, within BT0576 at 712.206 kb on - strand, within BT0576 at 714.331 kb on - strand at 714.399 kb on + strand at 714.406 kb on + strand at 714.676 kb on - strand, within BT0578 at 714.700 kb on + strand, within BT0578 at 714.743 kb on - strand, within BT0578 at 714.777 kb on + strand, within BT0578 at 714.778 kb on - strand, within BT0578 at 714.816 kb on + strand, within BT0578 at 714.917 kb on + strand, within BT0578 at 714.917 kb on + strand, within BT0578 at 714.923 kb on + strand, within BT0578
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 711,632 + -0.8 711,632 + -0.5 711,637 + -0.9 711,637 + +0.1 711,763 + -1.3 712,047 - BT0576 0.45 -2.5 712,206 - BT0576 0.67 +1.1 714,331 - +1.1 714,399 + -1.9 714,406 + +0.1 714,676 - BT0578 0.11 +0.1 714,700 + BT0578 0.12 +1.1 714,743 - BT0578 0.13 -1.9 714,777 + BT0578 0.15 -2.7 714,778 - BT0578 0.15 -1.3 714,816 + BT0578 0.16 -2.3 714,917 + BT0578 0.19 -1.5 714,917 + BT0578 0.19 -3.2 714,923 + BT0578 0.20 -1.3
Or see this region's nucleotide sequence