Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0250 and BT0251 overlap by 4 nucleotides BT0251 and BT0252 are separated by 244 nucleotides BT0252 and BT0253 are separated by 113 nucleotides
BT0250: BT0250 - dihydroorotase (DHOase) (NCBI ptt file), at 275,479 to 276,816
BT0250
BT0251: BT0251 - dolichol-phosphate mannosyltransferase (NCBI ptt file), at 276,813 to 277,556
BT0251
BT0252: BT0252 - transcription-repair coupling factor (NCBI ptt file), at 277,801 to 281,184
BT0252
BT0253: BT0253 - hypothetical protein (NCBI ptt file), at 281,298 to 282,143
BT0253
Position (kb) 277
278
279
280
281
282 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 277.603 kb on - strand at 277.618 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand at 277.801 kb on - strand at 277.891 kb on - strand at 278.292 kb on - strand, within BT0252 at 278.302 kb on + strand, within BT0252 at 278.312 kb on - strand, within BT0252 at 278.332 kb on + strand, within BT0252 at 278.332 kb on + strand, within BT0252 at 278.333 kb on - strand, within BT0252 at 278.658 kb on + strand, within BT0252 at 278.956 kb on - strand, within BT0252 at 278.956 kb on - strand, within BT0252 at 279.119 kb on - strand, within BT0252 at 279.183 kb on + strand, within BT0252 at 279.184 kb on - strand, within BT0252 at 279.268 kb on + strand, within BT0252 at 279.343 kb on - strand, within BT0252 at 279.719 kb on - strand, within BT0252 at 279.908 kb on + strand, within BT0252 at 279.909 kb on - strand, within BT0252 at 279.925 kb on - strand, within BT0252 at 279.957 kb on + strand, within BT0252 at 279.999 kb on - strand, within BT0252 at 280.048 kb on - strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.258 kb on + strand, within BT0252 at 280.282 kb on - strand, within BT0252 at 280.530 kb on - strand, within BT0252 at 280.756 kb on + strand, within BT0252 at 280.757 kb on - strand, within BT0252 at 280.757 kb on - strand, within BT0252 at 280.963 kb on - strand at 281.049 kb on + strand at 281.092 kb on - strand at 281.112 kb on + strand at 281.113 kb on - strand at 281.150 kb on + strand at 281.182 kb on + strand at 281.243 kb on + strand at 281.255 kb on + strand at 281.366 kb on + strand at 281.415 kb on - strand, within BT0253 at 281.424 kb on + strand, within BT0253 at 281.425 kb on - strand, within BT0253 at 281.431 kb on - strand, within BT0253 at 281.483 kb on - strand, within BT0253 at 281.543 kb on + strand, within BT0253 at 281.559 kb on + strand, within BT0253 at 281.560 kb on - strand, within BT0253 at 281.568 kb on + strand, within BT0253 at 281.569 kb on - strand, within BT0253 at 281.629 kb on + strand, within BT0253 at 281.764 kb on - strand, within BT0253 at 281.778 kb on - strand, within BT0253 at 281.832 kb on - strand, within BT0253 at 281.885 kb on + strand, within BT0253 at 281.975 kb on + strand, within BT0253 at 281.992 kb on + strand, within BT0253 at 282.014 kb on + strand, within BT0253 at 282.036 kb on - strand, within BT0253 at 282.068 kb on - strand at 282.072 kb on - strand at 282.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 277,603 - -2.4 277,618 - -1.5 277,801 - -3.1 277,801 - -0.6 277,801 - -3.8 277,891 - -2.0 278,292 - BT0252 0.15 -1.0 278,302 + BT0252 0.15 +0.0 278,312 - BT0252 0.15 -3.2 278,332 + BT0252 0.16 -2.1 278,332 + BT0252 0.16 -1.3 278,333 - BT0252 0.16 -1.6 278,658 + BT0252 0.25 -0.6 278,956 - BT0252 0.34 -0.6 278,956 - BT0252 0.34 -3.1 279,119 - BT0252 0.39 -1.6 279,183 + BT0252 0.41 +1.0 279,184 - BT0252 0.41 +0.0 279,268 + BT0252 0.43 -0.6 279,343 - BT0252 0.46 -0.9 279,719 - BT0252 0.57 -3.2 279,908 + BT0252 0.62 -5.0 279,909 - BT0252 0.62 -2.1 279,925 - BT0252 0.63 -2.3 279,957 + BT0252 0.64 -2.7 279,999 - BT0252 0.65 -5.7 280,048 - BT0252 0.66 -0.3 280,258 + BT0252 0.73 -1.0 280,258 + BT0252 0.73 -3.7 280,258 + BT0252 0.73 -3.5 280,258 + BT0252 0.73 -2.3 280,282 - BT0252 0.73 +0.5 280,530 - BT0252 0.81 -2.0 280,756 + BT0252 0.87 -1.8 280,757 - BT0252 0.87 -1.8 280,757 - BT0252 0.87 -3.1 280,963 - -2.7 281,049 + -2.7 281,092 - -2.7 281,112 + +1.0 281,113 - +0.0 281,150 + -2.4 281,182 + -1.0 281,243 + +0.0 281,255 + -1.6 281,366 + -1.0 281,415 - BT0253 0.14 -1.6 281,424 + BT0253 0.15 -1.4 281,425 - BT0253 0.15 -1.8 281,431 - BT0253 0.16 -1.8 281,483 - BT0253 0.22 -0.4 281,543 + BT0253 0.29 +0.0 281,559 + BT0253 0.31 +2.0 281,560 - BT0253 0.31 +1.0 281,568 + BT0253 0.32 +2.1 281,569 - BT0253 0.32 -1.8 281,629 + BT0253 0.39 +1.3 281,764 - BT0253 0.55 -0.2 281,778 - BT0253 0.57 -2.3 281,832 - BT0253 0.63 -0.9 281,885 + BT0253 0.69 -1.6 281,975 + BT0253 0.80 -0.5 281,992 + BT0253 0.82 +2.0 282,014 + BT0253 0.85 -1.3 282,036 - BT0253 0.87 -1.0 282,068 - -4.3 282,072 - +1.0 282,147 - +0.0
Or see this region's nucleotide sequence