Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09030 and PGA1_c09040 overlap by 4 nucleotides PGA1_c09040 and PGA1_c09050 overlap by 25 nucleotides PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides
PGA1_c09030: PGA1_c09030 - phosphomethylpyrimidine kinase ThiD, at 931,201 to 931,998
_c09030
PGA1_c09040: PGA1_c09040 - putative adenylyltransferase ThiF, at 931,995 to 932,984
_c09040
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
Position (kb)
932
933
934 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 931.996 kb on + strand at 932.004 kb on - strand at 932.004 kb on - strand at 932.009 kb on - strand at 932.016 kb on + strand at 932.068 kb on - strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.184 kb on - strand, within PGA1_c09040 at 932.207 kb on - strand, within PGA1_c09040 at 932.222 kb on - strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.275 kb on - strand, within PGA1_c09040 at 932.353 kb on - strand, within PGA1_c09040 at 932.436 kb on + strand, within PGA1_c09040 at 932.444 kb on - strand, within PGA1_c09040 at 932.496 kb on + strand, within PGA1_c09040 at 932.541 kb on - strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.580 kb on + strand, within PGA1_c09040 at 932.584 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.596 kb on - strand, within PGA1_c09040 at 932.708 kb on + strand, within PGA1_c09040 at 932.722 kb on + strand, within PGA1_c09040 at 932.757 kb on + strand, within PGA1_c09040 at 932.760 kb on - strand, within PGA1_c09040 at 932.786 kb on - strand, within PGA1_c09040 at 932.789 kb on + strand, within PGA1_c09040 at 932.800 kb on + strand, within PGA1_c09040 at 932.849 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.862 kb on - strand, within PGA1_c09040 at 932.981 kb on - strand at 932.981 kb on - strand at 932.981 kb on - strand at 933.114 kb on + strand, within PGA1_c09050 at 933.146 kb on + strand, within PGA1_c09050 at 933.154 kb on - strand, within PGA1_c09050 at 933.184 kb on - strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.213 kb on - strand, within PGA1_c09050 at 933.275 kb on + strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.312 kb on - strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 931,996 + +0.4 932,004 - -1.6 932,004 - -0.8 932,009 - -0.2 932,016 + -1.5 932,068 - +0.1 932,091 + -1.4 932,091 + -1.5 932,091 + +0.2 932,099 - PGA1_c09040 0.11 -0.5 932,099 - PGA1_c09040 0.11 -1.4 932,099 - PGA1_c09040 0.11 +0.9 932,099 - PGA1_c09040 0.11 +0.2 932,099 - PGA1_c09040 0.11 -1.7 932,184 - PGA1_c09040 0.19 -1.6 932,207 - PGA1_c09040 0.21 -1.6 932,222 - PGA1_c09040 0.23 -0.8 932,267 + PGA1_c09040 0.27 -1.0 932,267 + PGA1_c09040 0.27 -1.1 932,275 - PGA1_c09040 0.28 -1.0 932,353 - PGA1_c09040 0.36 -0.1 932,436 + PGA1_c09040 0.45 -1.2 932,444 - PGA1_c09040 0.45 +0.0 932,496 + PGA1_c09040 0.51 +0.4 932,541 - PGA1_c09040 0.55 -0.6 932,576 + PGA1_c09040 0.59 -1.8 932,576 + PGA1_c09040 0.59 -1.3 932,578 + PGA1_c09040 0.59 +0.6 932,578 + PGA1_c09040 0.59 +0.9 932,578 + PGA1_c09040 0.59 +0.2 932,578 + PGA1_c09040 0.59 +1.0 932,580 + PGA1_c09040 0.59 -2.0 932,584 - PGA1_c09040 0.59 -1.4 932,586 - PGA1_c09040 0.60 -0.9 932,586 - PGA1_c09040 0.60 -0.9 932,586 - PGA1_c09040 0.60 -2.2 932,588 + PGA1_c09040 0.60 -0.7 932,588 + PGA1_c09040 0.60 -0.3 932,596 - PGA1_c09040 0.61 +1.0 932,708 + PGA1_c09040 0.72 +1.2 932,722 + PGA1_c09040 0.73 -0.3 932,757 + PGA1_c09040 0.77 +0.1 932,760 - PGA1_c09040 0.77 -0.7 932,786 - PGA1_c09040 0.80 -1.5 932,789 + PGA1_c09040 0.80 -0.4 932,800 + PGA1_c09040 0.81 -3.2 932,849 + PGA1_c09040 0.86 -2.7 932,859 + PGA1_c09040 0.87 -0.9 932,859 + PGA1_c09040 0.87 -2.6 932,862 - PGA1_c09040 0.88 -0.1 932,981 - -0.7 932,981 - +0.2 932,981 - -0.1 933,114 + PGA1_c09050 0.26 -0.9 933,146 + PGA1_c09050 0.31 -2.3 933,154 - PGA1_c09050 0.33 +0.1 933,184 - PGA1_c09050 0.38 -0.2 933,186 + PGA1_c09050 0.38 -0.4 933,186 + PGA1_c09050 0.38 -0.8 933,213 - PGA1_c09050 0.43 -0.9 933,275 + PGA1_c09050 0.53 -1.6 933,283 - PGA1_c09050 0.54 -3.1 933,283 - PGA1_c09050 0.54 -1.4 933,312 - PGA1_c09050 0.59 -0.7 933,314 + PGA1_c09050 0.60 -0.6 933,314 + PGA1_c09050 0.60 -0.1 933,322 - PGA1_c09050 0.61 +1.9 933,322 - PGA1_c09050 0.61 -0.7 933,484 + PGA1_c09050 0.88 -0.7 933,488 + PGA1_c09050 0.89 -2.4 933,518 + -0.2 933,575 + +0.6 933,580 + -2.8 933,581 - -1.1 933,583 - -0.2 933,583 - -0.6 933,583 - -0.4 933,583 - -0.2 933,603 - -2.1 933,689 + PGA1_c09070 0.15 -1.1 933,702 - PGA1_c09070 0.16 -0.8 933,731 + PGA1_c09070 0.20 -1.9 933,731 + PGA1_c09070 0.20 -1.8 933,739 - PGA1_c09070 0.21 -1.0 933,767 + PGA1_c09070 0.25 -0.6 933,862 + PGA1_c09070 0.37 -2.2 933,865 - PGA1_c09070 0.38 -0.5 933,878 + PGA1_c09070 0.39 -1.4 933,881 - PGA1_c09070 0.40 -1.4 933,901 - PGA1_c09070 0.42 +0.2 933,948 + PGA1_c09070 0.48 +0.0 933,972 + PGA1_c09070 0.51 -0.4 933,978 - PGA1_c09070 0.52 -2.1 933,980 - PGA1_c09070 0.52 -0.4 934,004 + PGA1_c09070 0.56 -1.3 934,004 + PGA1_c09070 0.56 -0.4 934,004 + PGA1_c09070 0.56 -1.5 934,004 + PGA1_c09070 0.56 -0.4 934,004 + PGA1_c09070 0.56 -1.7 934,004 + PGA1_c09070 0.56 -0.9 934,004 + PGA1_c09070 0.56 -2.0 934,004 + PGA1_c09070 0.56 -1.1 934,012 - PGA1_c09070 0.57 +0.1 934,012 - PGA1_c09070 0.57 -0.8 934,012 - PGA1_c09070 0.57 -1.2 934,012 - PGA1_c09070 0.57 -1.4 934,012 - PGA1_c09070 0.57 -1.0 934,012 - PGA1_c09070 0.57 -1.2 934,012 - PGA1_c09070 0.57 -1.4 934,012 - PGA1_c09070 0.57 +0.6 934,012 - PGA1_c09070 0.57 +0.2 934,076 + PGA1_c09070 0.65 -2.1 934,084 - PGA1_c09070 0.66 -1.5 934,103 - PGA1_c09070 0.68 -2.2 934,103 - PGA1_c09070 0.68 -1.1 934,108 + PGA1_c09070 0.69 -0.9 934,142 + PGA1_c09070 0.74 -2.0 934,142 + PGA1_c09070 0.74 -0.7 934,150 - PGA1_c09070 0.75 -1.9 934,152 + PGA1_c09070 0.75 -0.4 934,152 + PGA1_c09070 0.75 -0.3 934,162 + PGA1_c09070 0.76 +0.1 934,275 + -1.0 934,275 - -1.0 934,306 - -1.6 934,311 + -0.7 934,311 + -2.2 934,311 + -0.5 934,319 - -1.0 934,339 - -0.7 934,354 - +0.6 934,364 + -1.6 934,472 - PGA1_c09090 0.63 -0.7 934,513 - PGA1_c09090 0.83 -1.0 934,529 - -0.6 934,539 + -1.0
Or see this region's nucleotide sequence