Experiment: PYE 30C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00222 and CCNA_00223 are separated by 8 nucleotides CCNA_00223 and CCNA_00224 overlap by 4 nucleotides CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides
CCNA_00222: CCNA_00222 - glucose-6-phosphate isomerase/glucose-6 phosphate 1-epimerase, at 238,241 to 239,860
_00222
CCNA_00223: CCNA_00223 - esterase lipase Family protein, at 239,869 to 240,651
_00223
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 239.814 kb on + strand at 239.878 kb on + strand at 239.929 kb on - strand at 239.955 kb on + strand, within CCNA_00223 at 240.014 kb on - strand, within CCNA_00223 at 240.028 kb on + strand, within CCNA_00223 at 240.036 kb on + strand, within CCNA_00223 at 240.036 kb on - strand, within CCNA_00223 at 240.042 kb on - strand, within CCNA_00223 at 240.050 kb on + strand, within CCNA_00223 at 240.058 kb on - strand, within CCNA_00223 at 240.061 kb on + strand, within CCNA_00223 at 240.061 kb on + strand, within CCNA_00223 at 240.069 kb on - strand, within CCNA_00223 at 240.074 kb on - strand, within CCNA_00223 at 240.082 kb on + strand, within CCNA_00223 at 240.105 kb on - strand, within CCNA_00223 at 240.152 kb on - strand, within CCNA_00223 at 240.152 kb on - strand, within CCNA_00223 at 240.162 kb on - strand, within CCNA_00223 at 240.223 kb on + strand, within CCNA_00223 at 240.269 kb on - strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.328 kb on + strand, within CCNA_00223 at 240.397 kb on + strand, within CCNA_00223 at 240.404 kb on + strand, within CCNA_00223 at 240.430 kb on - strand, within CCNA_00223 at 240.460 kb on - strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.498 kb on + strand, within CCNA_00223 at 240.503 kb on + strand, within CCNA_00223 at 240.511 kb on - strand, within CCNA_00223 at 240.516 kb on + strand, within CCNA_00223 at 240.524 kb on - strand, within CCNA_00223 at 240.524 kb on - strand, within CCNA_00223 at 240.531 kb on + strand, within CCNA_00223 at 240.547 kb on + strand, within CCNA_00223 at 240.555 kb on - strand, within CCNA_00223 at 240.674 kb on + strand at 240.674 kb on + strand at 240.674 kb on + strand at 240.682 kb on - strand at 240.682 kb on - strand at 240.702 kb on + strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.741 kb on + strand at 240.749 kb on - strand at 240.749 kb on - strand at 240.762 kb on + strand at 240.791 kb on - strand at 240.815 kb on + strand at 240.822 kb on - strand at 240.822 kb on - strand at 240.834 kb on - strand at 240.840 kb on + strand at 240.848 kb on - strand at 240.864 kb on + strand, within CCNA_00224 at 240.864 kb on + strand, within CCNA_00224 at 240.869 kb on + strand, within CCNA_00224 at 240.923 kb on + strand, within CCNA_00224 at 240.925 kb on + strand, within CCNA_00224 at 240.933 kb on - strand, within CCNA_00224 at 240.942 kb on + strand, within CCNA_00224 at 241.017 kb on + strand, within CCNA_00224 at 241.039 kb on - strand, within CCNA_00224 at 241.039 kb on - strand, within CCNA_00224 at 241.106 kb on - strand, within CCNA_00224 at 241.116 kb on - strand, within CCNA_00224 at 241.228 kb on + strand, within CCNA_00224 at 241.261 kb on + strand, within CCNA_00224 at 241.284 kb on - strand, within CCNA_00224 at 241.303 kb on + strand, within CCNA_00224 at 241.319 kb on + strand, within CCNA_00224 at 241.338 kb on - strand, within CCNA_00224 at 241.353 kb on - strand, within CCNA_00224 at 241.422 kb on - strand, within CCNA_00224 at 241.430 kb on - strand, within CCNA_00224 at 241.437 kb on + strand, within CCNA_00224 at 241.438 kb on + strand, within CCNA_00224 at 241.470 kb on - strand, within CCNA_00224 at 241.488 kb on + strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.553 kb on - strand, within CCNA_00224 at 241.565 kb on + strand, within CCNA_00224 at 241.565 kb on + strand, within CCNA_00224 at 241.567 kb on + strand, within CCNA_00224 at 241.573 kb on + strand, within CCNA_00224 at 241.575 kb on - strand, within CCNA_00224 at 241.581 kb on - strand, within CCNA_00224 at 241.581 kb on - strand, within CCNA_00224 at 241.585 kb on - strand, within CCNA_00224 at 241.598 kb on - strand, within CCNA_00224 at 241.626 kb on - strand, within CCNA_00224 at 241.636 kb on + strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.681 kb on + strand, within CCNA_00224 at 241.681 kb on + strand, within CCNA_00224 at 241.683 kb on - strand, within CCNA_00224 at 241.689 kb on - strand, within CCNA_00224 at 241.739 kb on - strand, within CCNA_00224 at 241.749 kb on - strand, within CCNA_00224 at 241.762 kb on + strand, within CCNA_00224 at 241.770 kb on - strand, within CCNA_00224 at 241.778 kb on + strand at 241.791 kb on + strand, within CCNA_00224 at 241.826 kb on - strand, within CCNA_00224 at 241.829 kb on - strand, within CCNA_00224 at 241.843 kb on + strand, within CCNA_00224 at 241.851 kb on - strand, within CCNA_00224 at 241.851 kb on - strand, within CCNA_00224 at 241.866 kb on + strand, within CCNA_00224 at 241.872 kb on + strand, within CCNA_00224 at 241.875 kb on - strand, within CCNA_00224 at 241.978 kb on - strand, within CCNA_00224 at 242.004 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on - strand, within CCNA_00224 at 242.170 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.188 kb on + strand, within CCNA_00224 at 242.196 kb on - strand, within CCNA_00224 at 242.209 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.250 kb on + strand, within CCNA_00224 at 242.264 kb on + strand, within CCNA_00224 at 242.300 kb on + strand, within CCNA_00224 at 242.305 kb on + strand, within CCNA_00224 at 242.338 kb on + strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.373 kb on - strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.565 kb on - strand at 242.573 kb on - strand at 242.587 kb on + strand at 242.587 kb on + strand at 242.595 kb on - strand at 242.600 kb on - strand at 242.643 kb on + strand at 242.656 kb on - strand at 242.675 kb on - strand at 242.683 kb on - strand at 242.704 kb on + strand at 242.715 kb on + strand at 242.716 kb on - strand at 242.718 kb on - strand at 242.723 kb on - strand at 242.723 kb on - strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.904 kb on + strand at 242.932 kb on + strand at 242.987 kb on + strand at 242.995 kb on - strand at 243.010 kb on + strand at 243.018 kb on - strand at 243.020 kb on + strand at 243.028 kb on - strand, within CCNA_00225 at 243.045 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 30C survival rep2 12 days remove 239,814 + +2.0 239,878 + -1.3 239,929 - +1.5 239,955 + CCNA_00223 0.11 -0.3 240,014 - CCNA_00223 0.19 +1.4 240,028 + CCNA_00223 0.20 +0.4 240,036 + CCNA_00223 0.21 -1.1 240,036 - CCNA_00223 0.21 -2.4 240,042 - CCNA_00223 0.22 +0.7 240,050 + CCNA_00223 0.23 -1.4 240,058 - CCNA_00223 0.24 +0.7 240,061 + CCNA_00223 0.25 -2.1 240,061 + CCNA_00223 0.25 +0.5 240,069 - CCNA_00223 0.26 -1.0 240,074 - CCNA_00223 0.26 -1.7 240,082 + CCNA_00223 0.27 +1.1 240,105 - CCNA_00223 0.30 +1.8 240,152 - CCNA_00223 0.36 -0.9 240,152 - CCNA_00223 0.36 +0.2 240,162 - CCNA_00223 0.37 +0.4 240,223 + CCNA_00223 0.45 -2.4 240,269 - CCNA_00223 0.51 -1.6 240,312 + CCNA_00223 0.57 -0.8 240,312 + CCNA_00223 0.57 -0.3 240,312 + CCNA_00223 0.57 +0.6 240,318 + CCNA_00223 0.57 -0.6 240,320 - CCNA_00223 0.58 -0.3 240,320 - CCNA_00223 0.58 +0.8 240,320 - CCNA_00223 0.58 +1.8 240,320 - CCNA_00223 0.58 +0.2 240,320 - CCNA_00223 0.58 +1.4 240,326 - CCNA_00223 0.58 -0.5 240,328 + CCNA_00223 0.59 +0.3 240,397 + CCNA_00223 0.67 -2.9 240,404 + CCNA_00223 0.68 -1.9 240,430 - CCNA_00223 0.72 -0.6 240,460 - CCNA_00223 0.75 -0.7 240,468 + CCNA_00223 0.77 -0.3 240,468 + CCNA_00223 0.77 +1.0 240,468 + CCNA_00223 0.77 -0.1 240,498 + CCNA_00223 0.80 -0.4 240,503 + CCNA_00223 0.81 -1.5 240,511 - CCNA_00223 0.82 -0.3 240,516 + CCNA_00223 0.83 +0.3 240,524 - CCNA_00223 0.84 -1.8 240,524 - CCNA_00223 0.84 -0.0 240,531 + CCNA_00223 0.85 -0.4 240,547 + CCNA_00223 0.87 +1.2 240,555 - CCNA_00223 0.88 +1.6 240,674 + -0.6 240,674 + +1.0 240,674 + +2.2 240,682 - -0.2 240,682 - +0.0 240,702 + -2.7 240,710 - +0.7 240,710 - -1.0 240,710 - +0.4 240,710 - -0.5 240,710 - +1.4 240,741 + -1.5 240,749 - -0.7 240,749 - -0.8 240,762 + -0.9 240,791 - +1.0 240,815 + -0.0 240,822 - -2.2 240,822 - -1.5 240,834 - -1.1 240,840 + -1.1 240,848 - +1.8 240,864 + CCNA_00224 0.11 +1.4 240,864 + CCNA_00224 0.11 +0.7 240,869 + CCNA_00224 0.11 -0.8 240,923 + CCNA_00224 0.13 -0.9 240,925 + CCNA_00224 0.14 -0.9 240,933 - CCNA_00224 0.14 -0.1 240,942 + CCNA_00224 0.14 -0.3 241,017 + CCNA_00224 0.18 -1.2 241,039 - CCNA_00224 0.19 +0.6 241,039 - CCNA_00224 0.19 -0.6 241,106 - CCNA_00224 0.22 -0.8 241,116 - CCNA_00224 0.23 +3.2 241,228 + CCNA_00224 0.28 +1.4 241,261 + CCNA_00224 0.30 -2.1 241,284 - CCNA_00224 0.31 +0.7 241,303 + CCNA_00224 0.32 +2.4 241,319 + CCNA_00224 0.33 -0.0 241,338 - CCNA_00224 0.34 -2.1 241,353 - CCNA_00224 0.34 -1.2 241,422 - CCNA_00224 0.38 -1.0 241,430 - CCNA_00224 0.38 +0.8 241,437 + CCNA_00224 0.39 +3.1 241,438 + CCNA_00224 0.39 -1.4 241,470 - CCNA_00224 0.40 -0.5 241,488 + CCNA_00224 0.41 +0.2 241,548 - CCNA_00224 0.44 -0.6 241,548 - CCNA_00224 0.44 -1.8 241,548 - CCNA_00224 0.44 +1.9 241,553 - CCNA_00224 0.44 -0.6 241,565 + CCNA_00224 0.45 -0.3 241,565 + CCNA_00224 0.45 +0.7 241,567 + CCNA_00224 0.45 -1.0 241,573 + CCNA_00224 0.45 -2.1 241,575 - CCNA_00224 0.45 -0.9 241,581 - CCNA_00224 0.46 -2.4 241,581 - CCNA_00224 0.46 +2.2 241,585 - CCNA_00224 0.46 -2.6 241,598 - CCNA_00224 0.46 -1.4 241,626 - CCNA_00224 0.48 +0.8 241,636 + CCNA_00224 0.48 +0.4 241,644 + CCNA_00224 0.49 -0.9 241,644 + CCNA_00224 0.49 -2.3 241,652 - CCNA_00224 0.49 -0.4 241,652 - CCNA_00224 0.49 -1.7 241,652 - CCNA_00224 0.49 -0.2 241,681 + CCNA_00224 0.50 -0.5 241,681 + CCNA_00224 0.50 -3.7 241,683 - CCNA_00224 0.51 -0.0 241,689 - CCNA_00224 0.51 -0.3 241,739 - CCNA_00224 0.53 +0.4 241,749 - CCNA_00224 0.54 -1.9 241,762 + CCNA_00224 0.54 +0.2 241,770 - CCNA_00224 0.55 +0.7 241,778 + +1.4 241,791 + CCNA_00224 0.56 -0.3 241,826 - CCNA_00224 0.58 -1.4 241,829 - CCNA_00224 0.58 +0.3 241,843 + CCNA_00224 0.58 -0.2 241,851 - CCNA_00224 0.59 +0.7 241,851 - CCNA_00224 0.59 -3.0 241,866 + CCNA_00224 0.60 -0.9 241,872 + CCNA_00224 0.60 +1.0 241,875 - CCNA_00224 0.60 -0.7 241,978 - CCNA_00224 0.65 -0.8 242,004 + CCNA_00224 0.66 -0.7 242,164 + CCNA_00224 0.74 -2.1 242,164 + CCNA_00224 0.74 +1.0 242,164 - CCNA_00224 0.74 +1.5 242,170 - CCNA_00224 0.74 -0.5 242,172 - CCNA_00224 0.74 +0.5 242,172 - CCNA_00224 0.74 -1.9 242,188 + CCNA_00224 0.75 -0.0 242,196 - CCNA_00224 0.76 -0.7 242,209 + CCNA_00224 0.76 -0.6 242,224 - CCNA_00224 0.77 +0.5 242,224 - CCNA_00224 0.77 -0.7 242,250 + CCNA_00224 0.78 -1.2 242,264 + CCNA_00224 0.79 +1.4 242,300 + CCNA_00224 0.81 -1.4 242,305 + CCNA_00224 0.81 -1.3 242,338 + CCNA_00224 0.83 -2.4 242,343 + CCNA_00224 0.83 +1.0 242,373 - CCNA_00224 0.84 -0.8 242,428 - CCNA_00224 0.87 -1.6 242,441 - CCNA_00224 0.88 -0.3 242,441 - CCNA_00224 0.88 -2.1 242,481 + CCNA_00224 0.90 -2.1 242,489 - -0.7 242,527 + -1.4 242,527 + +1.0 242,527 + +0.6 242,527 + +1.4 242,527 + +0.3 242,527 + +0.4 242,527 + -0.0 242,527 + -0.2 242,527 + -0.8 242,527 + +2.0 242,527 + -0.4 242,535 - -0.7 242,535 - -1.9 242,535 - +0.2 242,535 - -1.6 242,535 - +0.7 242,565 - -1.7 242,573 - -1.4 242,587 + +2.0 242,587 + -0.9 242,595 - -0.8 242,600 - -2.5 242,643 + +0.7 242,656 - -0.6 242,675 - +1.5 242,683 - -0.9 242,704 + +2.0 242,715 + -0.6 242,716 - -1.3 242,718 - +1.2 242,723 - -0.2 242,723 - -1.1 242,892 + -1.6 242,892 + +0.2 242,892 + +0.8 242,892 + +1.0 242,892 + -0.0 242,892 + -0.0 242,900 - +0.5 242,900 - -1.2 242,900 - -1.5 242,900 - -1.2 242,900 - -0.3 242,900 - +0.4 242,900 - -1.3 242,900 - -1.4 242,900 - -3.6 242,904 + -0.6 242,932 + -0.6 242,987 + +1.7 242,995 - -1.7 243,010 + +1.9 243,018 - +0.2 243,020 + -1.2 243,028 - CCNA_00225 0.12 -1.0 243,045 + CCNA_00225 0.64 -2.8 243,050 + CCNA_00225 0.79 -0.2 243,050 + CCNA_00225 0.79 -0.5 243,068 - -0.6 243,083 + -2.8 243,091 - +0.7 243,154 + -1.4 243,162 - +1.5 243,162 - -1.2 243,162 - -0.6 243,162 - -0.2 243,164 + -0.3 243,164 + -0.0 243,164 + +2.1 243,198 + CCNA_00226 0.15 +1.9 243,198 + CCNA_00226 0.15 -3.6 243,202 - CCNA_00226 0.16 +0.7 243,205 + CCNA_00226 0.17 -0.4 243,206 - CCNA_00226 0.18 -1.5 243,213 + CCNA_00226 0.20 +1.1 243,246 - CCNA_00226 0.32 -0.4 243,259 + CCNA_00226 0.37 +0.7 243,275 + CCNA_00226 0.43 -0.0 243,275 + CCNA_00226 0.43 +3.3 243,276 - CCNA_00226 0.43 -2.4 243,283 - CCNA_00226 0.46 +0.1 243,304 + CCNA_00226 0.53 +1.0 243,400 + CCNA_00226 0.88 +1.0 243,424 - -0.7 243,431 - -0.8 243,434 + -0.9 243,449 + -0.4 243,449 + -0.1 243,449 + -2.1 243,457 - -1.2 243,457 - -0.5 243,457 - +1.5 243,457 - -1.9 243,462 + +0.3 243,470 - -2.6 243,479 - +0.5 243,489 - +0.7 243,489 - +1.4 243,495 - +0.0 243,528 - -1.2 243,539 + -0.2 243,547 - CCNA_00227 0.10 -1.5 243,557 - CCNA_00227 0.12 -2.0 243,557 - CCNA_00227 0.12 -3.2 243,557 - CCNA_00227 0.12 -2.4 243,598 - CCNA_00227 0.19 -2.0 243,620 + CCNA_00227 0.23 +1.0 243,647 - CCNA_00227 0.28 +0.1 243,647 - CCNA_00227 0.28 -0.2 243,673 - CCNA_00227 0.32 -2.4
Or see this region's nucleotide sequence