Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS13700

Experiment: reconstituted MRS with Yacontrol-prebiotic 0.5 %w/v

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS13695 and BBR_RS13700 are separated by 130 nucleotidesBBR_RS13700 and BBR_RS13705 are separated by 68 nucleotidesBBR_RS13705 and BBR_RS13710 are separated by 126 nucleotides BBR_RS13695: BBR_RS13695 - glutamate racemase, at 846,186 to 846,980 _RS13695 BBR_RS13700: BBR_RS13700 - diaminopimelate epimerase, at 847,111 to 848,010 _RS13700 BBR_RS13705: BBR_RS13705 - Conserved hypothetical membrane spanning protein, contains a vitamin K epoxide reductase domain, at 848,079 to 848,798 _RS13705 BBR_RS13710: BBR_RS13710 - PHP domain-containing protein, at 848,925 to 849,818 _RS13710 Position (kb) 847 848 849Strain fitness (log2 ratio) -3 -2 -1 0 1at 846.117 kb on + strandat 846.173 kb on - strandat 846.208 kb on - strandat 846.212 kb on + strandat 846.220 kb on - strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.043 kb on + strandat 847.051 kb on - strandat 847.058 kb on + strandat 847.077 kb on + strandat 847.098 kb on - strandat 847.098 kb on - strandat 847.169 kb on + strandat 847.216 kb on - strand, within BBR_RS13700at 847.252 kb on + strand, within BBR_RS13700at 847.266 kb on + strand, within BBR_RS13700at 847.266 kb on + strand, within BBR_RS13700at 847.269 kb on - strand, within BBR_RS13700at 847.283 kb on + strand, within BBR_RS13700at 847.288 kb on + strand, within BBR_RS13700at 847.338 kb on - strand, within BBR_RS13700at 847.360 kb on + strand, within BBR_RS13700at 847.412 kb on - strand, within BBR_RS13700at 847.419 kb on + strand, within BBR_RS13700at 847.419 kb on + strand, within BBR_RS13700at 847.419 kb on + strand, within BBR_RS13700at 847.419 kb on + strand, within BBR_RS13700at 847.447 kb on - strand, within BBR_RS13700at 847.480 kb on + strand, within BBR_RS13700at 847.489 kb on + strand, within BBR_RS13700at 847.500 kb on + strand, within BBR_RS13700at 847.507 kb on - strand, within BBR_RS13700at 847.514 kb on + strand, within BBR_RS13700at 847.514 kb on + strand, within BBR_RS13700at 847.522 kb on - strand, within BBR_RS13700at 847.522 kb on - strand, within BBR_RS13700at 847.568 kb on + strand, within BBR_RS13700at 847.568 kb on + strand, within BBR_RS13700at 847.568 kb on + strand, within BBR_RS13700at 847.576 kb on - strand, within BBR_RS13700at 847.576 kb on - strand, within BBR_RS13700at 847.639 kb on - strand, within BBR_RS13700at 847.688 kb on - strand, within BBR_RS13700at 847.713 kb on + strand, within BBR_RS13700at 847.726 kb on - strand, within BBR_RS13700at 847.771 kb on + strand, within BBR_RS13700at 847.783 kb on + strand, within BBR_RS13700at 847.826 kb on + strand, within BBR_RS13700at 847.834 kb on - strand, within BBR_RS13700at 847.852 kb on - strand, within BBR_RS13700at 847.854 kb on + strand, within BBR_RS13700at 847.870 kb on + strand, within BBR_RS13700at 847.878 kb on + strand, within BBR_RS13700at 847.900 kb on - strand, within BBR_RS13700at 847.925 kb on + strandat 847.952 kb on + strandat 847.953 kb on + strandat 847.974 kb on - strandat 847.993 kb on + strandat 848.003 kb on + strandat 848.006 kb on - strandat 848.020 kb on + strandat 848.101 kb on - strandat 848.103 kb on - strandat 848.145 kb on - strandat 848.291 kb on - strand, within BBR_RS13705at 848.291 kb on - strand, within BBR_RS13705at 848.291 kb on - strand, within BBR_RS13705at 848.305 kb on + strand, within BBR_RS13705at 848.305 kb on + strand, within BBR_RS13705at 848.321 kb on - strand, within BBR_RS13705at 848.350 kb on + strand, within BBR_RS13705at 848.368 kb on - strand, within BBR_RS13705at 848.387 kb on - strand, within BBR_RS13705at 848.388 kb on + strand, within BBR_RS13705at 848.391 kb on - strand, within BBR_RS13705at 848.396 kb on - strand, within BBR_RS13705at 848.481 kb on - strand, within BBR_RS13705at 848.505 kb on - strand, within BBR_RS13705at 848.610 kb on + strand, within BBR_RS13705at 848.618 kb on - strand, within BBR_RS13705at 848.694 kb on + strand, within BBR_RS13705at 848.707 kb on - strand, within BBR_RS13705at 848.721 kb on + strand, within BBR_RS13705at 848.728 kb on - strandat 848.819 kb on - strandat 848.927 kb on + strandat 848.938 kb on - strandat 848.940 kb on + strandat 848.940 kb on - strandat 848.940 kb on - strandat 848.953 kb on + strandat 848.953 kb on + strandat 848.963 kb on + strandat 848.971 kb on - strandat 848.971 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Yacontrol-prebiotic 0.5 %w/v
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846,117 + -0.4
846,173 - +0.1
846,208 - -0.6
846,212 + -1.1
846,220 - +1.0
847,043 + -0.8
847,043 + -1.0
847,043 + -0.2
847,043 + +0.1
847,043 + -0.8
847,043 + -0.1
847,043 + -0.2
847,051 - +0.4
847,058 + +0.0
847,077 + +0.9
847,098 - +0.0
847,098 - -2.3
847,169 + -0.5
847,216 - BBR_RS13700 0.12 -0.2
847,252 + BBR_RS13700 0.16 +0.4
847,266 + BBR_RS13700 0.17 +0.2
847,266 + BBR_RS13700 0.17 -0.0
847,269 - BBR_RS13700 0.18 -0.4
847,283 + BBR_RS13700 0.19 +0.3
847,288 + BBR_RS13700 0.20 -0.6
847,338 - BBR_RS13700 0.25 +0.1
847,360 + BBR_RS13700 0.28 -0.4
847,412 - BBR_RS13700 0.33 -0.4
847,419 + BBR_RS13700 0.34 -1.0
847,419 + BBR_RS13700 0.34 -0.7
847,419 + BBR_RS13700 0.34 -0.1
847,419 + BBR_RS13700 0.34 -0.4
847,447 - BBR_RS13700 0.37 -2.3
847,480 + BBR_RS13700 0.41 +0.1
847,489 + BBR_RS13700 0.42 -0.5
847,500 + BBR_RS13700 0.43 +0.4
847,507 - BBR_RS13700 0.44 -0.5
847,514 + BBR_RS13700 0.45 +0.1
847,514 + BBR_RS13700 0.45 -0.1
847,522 - BBR_RS13700 0.46 -2.8
847,522 - BBR_RS13700 0.46 +0.1
847,568 + BBR_RS13700 0.51 +0.4
847,568 + BBR_RS13700 0.51 +0.8
847,568 + BBR_RS13700 0.51 +0.1
847,576 - BBR_RS13700 0.52 -0.1
847,576 - BBR_RS13700 0.52 +0.4
847,639 - BBR_RS13700 0.59 +0.0
847,688 - BBR_RS13700 0.64 -0.9
847,713 + BBR_RS13700 0.67 -0.5
847,726 - BBR_RS13700 0.68 -0.9
847,771 + BBR_RS13700 0.73 +0.4
847,783 + BBR_RS13700 0.75 +1.0
847,826 + BBR_RS13700 0.79 +0.6
847,834 - BBR_RS13700 0.80 +0.1
847,852 - BBR_RS13700 0.82 -0.1
847,854 + BBR_RS13700 0.83 +0.7
847,870 + BBR_RS13700 0.84 +0.2
847,878 + BBR_RS13700 0.85 -0.1
847,900 - BBR_RS13700 0.88 +0.9
847,925 + +0.6
847,952 + +0.4
847,953 + +0.1
847,974 - -0.2
847,993 + -0.2
848,003 + +0.3
848,006 - -0.5
848,020 + +0.2
848,101 - -0.3
848,103 - -0.0
848,145 - -0.6
848,291 - BBR_RS13705 0.29 -0.4
848,291 - BBR_RS13705 0.29 -0.5
848,291 - BBR_RS13705 0.29 +0.2
848,305 + BBR_RS13705 0.31 +0.3
848,305 + BBR_RS13705 0.31 -0.1
848,321 - BBR_RS13705 0.34 -0.5
848,350 + BBR_RS13705 0.38 +0.3
848,368 - BBR_RS13705 0.40 -0.5
848,387 - BBR_RS13705 0.43 -0.7
848,388 + BBR_RS13705 0.43 +0.4
848,391 - BBR_RS13705 0.43 -0.7
848,396 - BBR_RS13705 0.44 +0.8
848,481 - BBR_RS13705 0.56 -1.4
848,505 - BBR_RS13705 0.59 +0.0
848,610 + BBR_RS13705 0.74 +0.2
848,618 - BBR_RS13705 0.75 -0.2
848,694 + BBR_RS13705 0.85 +0.6
848,707 - BBR_RS13705 0.87 -0.8
848,721 + BBR_RS13705 0.89 +0.3
848,728 - +0.9
848,819 - +0.3
848,927 + +0.5
848,938 - +0.1
848,940 + +0.8
848,940 - +0.0
848,940 - +0.0
848,953 + -0.1
848,953 + +0.4
848,963 + +1.1
848,971 - +0.6
848,971 - +0.2

Or see this region's nucleotide sequence