Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1052 and Dshi_1053 are separated by 3 nucleotides Dshi_1053 and Dshi_1054 are separated by 10 nucleotides Dshi_1054 and Dshi_1055 are separated by 8 nucleotides
Dshi_1052: Dshi_1052 - Spermidine/putrescine-binding periplasmic protein-like protein (RefSeq), at 1,085,608 to 1,086,777
_1052
Dshi_1053: Dshi_1053 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 1,086,781 to 1,087,698
_1053
Dshi_1054: Dshi_1054 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 1,087,709 to 1,088,599
_1054
Dshi_1055: Dshi_1055 - ABC transporter related (RefSeq), at 1,088,608 to 1,089,708
_1055
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-2
-1
0
1 at 1085.830 kb on + strand, within Dshi_1052 at 1085.872 kb on + strand, within Dshi_1052 at 1085.978 kb on + strand, within Dshi_1052 at 1085.986 kb on - strand, within Dshi_1052 at 1086.075 kb on - strand, within Dshi_1052 at 1086.075 kb on - strand, within Dshi_1052 at 1086.104 kb on + strand, within Dshi_1052 at 1086.104 kb on + strand, within Dshi_1052 at 1086.169 kb on + strand, within Dshi_1052 at 1086.191 kb on + strand, within Dshi_1052 at 1086.191 kb on + strand, within Dshi_1052 at 1086.297 kb on + strand, within Dshi_1052 at 1086.337 kb on - strand, within Dshi_1052 at 1086.363 kb on - strand, within Dshi_1052 at 1086.379 kb on - strand, within Dshi_1052 at 1086.443 kb on + strand, within Dshi_1052 at 1086.445 kb on + strand, within Dshi_1052 at 1086.459 kb on - strand, within Dshi_1052 at 1086.685 kb on + strand at 1086.825 kb on + strand at 1086.888 kb on + strand, within Dshi_1053 at 1086.890 kb on + strand, within Dshi_1053 at 1087.099 kb on + strand, within Dshi_1053 at 1087.132 kb on - strand, within Dshi_1053 at 1087.147 kb on - strand, within Dshi_1053 at 1087.207 kb on + strand, within Dshi_1053 at 1087.213 kb on - strand, within Dshi_1053 at 1087.242 kb on + strand, within Dshi_1053 at 1087.281 kb on + strand, within Dshi_1053 at 1087.281 kb on + strand, within Dshi_1053 at 1087.281 kb on + strand, within Dshi_1053 at 1087.281 kb on + strand, within Dshi_1053 at 1087.289 kb on - strand, within Dshi_1053 at 1087.289 kb on - strand, within Dshi_1053 at 1087.399 kb on + strand, within Dshi_1053 at 1087.407 kb on - strand, within Dshi_1053 at 1087.461 kb on - strand, within Dshi_1053 at 1087.560 kb on - strand, within Dshi_1053 at 1087.596 kb on + strand, within Dshi_1053 at 1087.596 kb on + strand, within Dshi_1053 at 1087.641 kb on + strand at 1087.697 kb on - strand at 1087.795 kb on + strand at 1087.951 kb on - strand, within Dshi_1054 at 1087.991 kb on + strand, within Dshi_1054 at 1088.151 kb on + strand, within Dshi_1054 at 1088.166 kb on + strand, within Dshi_1054 at 1088.234 kb on - strand, within Dshi_1054 at 1088.251 kb on + strand, within Dshi_1054 at 1088.259 kb on - strand, within Dshi_1054 at 1088.320 kb on - strand, within Dshi_1054 at 1088.327 kb on + strand, within Dshi_1054 at 1088.382 kb on + strand, within Dshi_1054 at 1088.400 kb on - strand, within Dshi_1054 at 1088.467 kb on - strand, within Dshi_1054 at 1088.536 kb on + strand at 1088.557 kb on - strand at 1088.557 kb on - strand at 1088.557 kb on - strand at 1088.557 kb on - strand at 1088.569 kb on + strand at 1088.569 kb on + strand at 1088.569 kb on + strand at 1088.569 kb on + strand at 1088.569 kb on + strand at 1088.577 kb on - strand at 1088.577 kb on - strand at 1088.577 kb on - strand at 1088.577 kb on - strand at 1088.577 kb on - strand at 1088.626 kb on - strand at 1088.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 1,085,830 + Dshi_1052 0.19 +0.3 1,085,872 + Dshi_1052 0.23 +0.0 1,085,978 + Dshi_1052 0.32 +1.5 1,085,986 - Dshi_1052 0.32 -0.8 1,086,075 - Dshi_1052 0.40 -0.5 1,086,075 - Dshi_1052 0.40 -2.0 1,086,104 + Dshi_1052 0.42 -0.2 1,086,104 + Dshi_1052 0.42 -0.9 1,086,169 + Dshi_1052 0.48 +0.8 1,086,191 + Dshi_1052 0.50 -0.7 1,086,191 + Dshi_1052 0.50 -1.2 1,086,297 + Dshi_1052 0.59 -0.1 1,086,337 - Dshi_1052 0.62 +0.2 1,086,363 - Dshi_1052 0.65 +0.1 1,086,379 - Dshi_1052 0.66 +0.3 1,086,443 + Dshi_1052 0.71 +0.6 1,086,445 + Dshi_1052 0.72 -0.7 1,086,459 - Dshi_1052 0.73 -0.5 1,086,685 + +0.5 1,086,825 + -0.1 1,086,888 + Dshi_1053 0.12 +0.1 1,086,890 + Dshi_1053 0.12 -0.4 1,087,099 + Dshi_1053 0.35 +0.0 1,087,132 - Dshi_1053 0.38 +0.2 1,087,147 - Dshi_1053 0.40 +0.5 1,087,207 + Dshi_1053 0.46 -0.4 1,087,213 - Dshi_1053 0.47 +1.5 1,087,242 + Dshi_1053 0.50 +0.5 1,087,281 + Dshi_1053 0.54 +0.6 1,087,281 + Dshi_1053 0.54 -1.1 1,087,281 + Dshi_1053 0.54 -0.1 1,087,281 + Dshi_1053 0.54 +0.5 1,087,289 - Dshi_1053 0.55 -0.1 1,087,289 - Dshi_1053 0.55 +1.0 1,087,399 + Dshi_1053 0.67 -0.3 1,087,407 - Dshi_1053 0.68 -0.4 1,087,461 - Dshi_1053 0.74 +0.3 1,087,560 - Dshi_1053 0.85 +0.3 1,087,596 + Dshi_1053 0.89 +0.3 1,087,596 + Dshi_1053 0.89 +0.3 1,087,641 + -0.1 1,087,697 - +0.9 1,087,795 + -0.1 1,087,951 - Dshi_1054 0.27 +0.2 1,087,991 + Dshi_1054 0.32 +0.4 1,088,151 + Dshi_1054 0.50 -0.1 1,088,166 + Dshi_1054 0.51 -0.3 1,088,234 - Dshi_1054 0.59 +0.0 1,088,251 + Dshi_1054 0.61 -0.9 1,088,259 - Dshi_1054 0.62 -0.2 1,088,320 - Dshi_1054 0.69 +1.2 1,088,327 + Dshi_1054 0.69 +0.5 1,088,382 + Dshi_1054 0.76 +0.3 1,088,400 - Dshi_1054 0.78 +0.7 1,088,467 - Dshi_1054 0.85 -0.0 1,088,536 + +0.0 1,088,557 - -0.5 1,088,557 - -0.6 1,088,557 - +0.2 1,088,557 - -0.8 1,088,569 + -0.7 1,088,569 + +0.5 1,088,569 + -0.1 1,088,569 + -0.0 1,088,569 + -0.8 1,088,577 - +0.1 1,088,577 - -0.7 1,088,577 - +0.4 1,088,577 - -0.6 1,088,577 - +0.4 1,088,626 - -1.5 1,088,656 + -0.6
Or see this region's nucleotide sequence