Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0587 and Dshi_0588 are separated by 77 nucleotides Dshi_0588 and Dshi_0589 overlap by 4 nucleotides Dshi_0589 and Dshi_0590 are separated by 33 nucleotides Dshi_0590 and Dshi_0591 are separated by 9 nucleotides
Dshi_0586: Dshi_0586 - permease (RefSeq), at 577,452 to 578,498
_0586
Dshi_0587: Dshi_0587 - putative transcriptional regulator, ArsR family (RefSeq), at 578,470 to 578,814
_0587
Dshi_0588: Dshi_0588 - uracil-DNA glycosylase superfamily (RefSeq), at 578,892 to 579,500
_0588
Dshi_0589: Dshi_0589 - hypothetical protein (RefSeq), at 579,497 to 580,282
_0589
Dshi_0590: Dshi_0590 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 580,316 to 581,251
_0590
Dshi_0591: Dshi_0591 - rod shape-determining protein RodA (RefSeq), at 581,261 to 582,400
_0591
Position (kb) 579
580
581 Strain fitness (log2 ratio) -2
-1
0
1 at 578.584 kb on - strand, within Dshi_0587 at 578.628 kb on + strand, within Dshi_0587 at 578.701 kb on - strand, within Dshi_0587 at 578.884 kb on - strand at 579.090 kb on - strand, within Dshi_0588 at 579.109 kb on - strand, within Dshi_0588 at 579.232 kb on - strand, within Dshi_0588 at 579.232 kb on - strand, within Dshi_0588 at 579.232 kb on - strand, within Dshi_0588 at 579.232 kb on - strand, within Dshi_0588 at 579.235 kb on + strand, within Dshi_0588 at 579.322 kb on + strand, within Dshi_0588 at 579.418 kb on + strand, within Dshi_0588 at 579.426 kb on - strand, within Dshi_0588 at 579.427 kb on - strand, within Dshi_0588 at 579.488 kb on + strand at 579.488 kb on + strand at 579.488 kb on + strand at 579.522 kb on - strand at 579.633 kb on - strand, within Dshi_0589 at 579.645 kb on + strand, within Dshi_0589 at 579.655 kb on - strand, within Dshi_0589 at 579.675 kb on + strand, within Dshi_0589 at 579.675 kb on + strand, within Dshi_0589 at 579.738 kb on + strand, within Dshi_0589 at 579.887 kb on - strand, within Dshi_0589 at 579.994 kb on + strand, within Dshi_0589 at 580.040 kb on + strand, within Dshi_0589 at 580.068 kb on + strand, within Dshi_0589 at 580.082 kb on - strand, within Dshi_0589 at 580.129 kb on - strand, within Dshi_0589 at 580.195 kb on - strand, within Dshi_0589 at 580.244 kb on - strand at 580.255 kb on - strand at 580.440 kb on + strand, within Dshi_0590 at 580.443 kb on - strand, within Dshi_0590 at 580.523 kb on - strand, within Dshi_0590 at 580.815 kb on + strand, within Dshi_0590 at 580.899 kb on + strand, within Dshi_0590 at 580.928 kb on + strand, within Dshi_0590 at 580.933 kb on + strand, within Dshi_0590 at 580.970 kb on - strand, within Dshi_0590 at 580.991 kb on + strand, within Dshi_0590 at 580.991 kb on + strand, within Dshi_0590 at 580.999 kb on - strand, within Dshi_0590 at 581.148 kb on + strand, within Dshi_0590 at 581.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 578,584 - Dshi_0587 0.33 +0.2 578,628 + Dshi_0587 0.46 +1.0 578,701 - Dshi_0587 0.67 +1.0 578,884 - +0.5 579,090 - Dshi_0588 0.33 +0.1 579,109 - Dshi_0588 0.36 -0.8 579,232 - Dshi_0588 0.56 +1.1 579,232 - Dshi_0588 0.56 +0.8 579,232 - Dshi_0588 0.56 +0.3 579,232 - Dshi_0588 0.56 +0.7 579,235 + Dshi_0588 0.56 -0.1 579,322 + Dshi_0588 0.71 -0.2 579,418 + Dshi_0588 0.86 +0.5 579,426 - Dshi_0588 0.88 -0.0 579,427 - Dshi_0588 0.88 -0.0 579,488 + -0.8 579,488 + +0.0 579,488 + +1.0 579,522 - +0.4 579,633 - Dshi_0589 0.17 +0.5 579,645 + Dshi_0589 0.19 -0.5 579,655 - Dshi_0589 0.20 -2.2 579,675 + Dshi_0589 0.23 +0.3 579,675 + Dshi_0589 0.23 -0.3 579,738 + Dshi_0589 0.31 +0.2 579,887 - Dshi_0589 0.50 -0.5 579,994 + Dshi_0589 0.63 -0.4 580,040 + Dshi_0589 0.69 -0.6 580,068 + Dshi_0589 0.73 +1.0 580,082 - Dshi_0589 0.74 -0.2 580,129 - Dshi_0589 0.80 -1.0 580,195 - Dshi_0589 0.89 +1.2 580,244 - -0.4 580,255 - -2.3 580,440 + Dshi_0590 0.13 +0.0 580,443 - Dshi_0590 0.14 -0.5 580,523 - Dshi_0590 0.22 -0.0 580,815 + Dshi_0590 0.53 +0.9 580,899 + Dshi_0590 0.62 -0.4 580,928 + Dshi_0590 0.65 -2.4 580,933 + Dshi_0590 0.66 -0.2 580,970 - Dshi_0590 0.70 -0.8 580,991 + Dshi_0590 0.72 -0.8 580,991 + Dshi_0590 0.72 -0.1 580,999 - Dshi_0590 0.73 -0.2 581,148 + Dshi_0590 0.89 -1.1 581,181 - +0.4
Or see this region's nucleotide sequence