Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21900

Experiment: L-Histidine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21890 and BWI76_RS21895 are separated by 9 nucleotidesBWI76_RS21895 and BWI76_RS21900 are separated by 24 nucleotidesBWI76_RS21900 and BWI76_RS21905 are separated by 0 nucleotidesBWI76_RS21905 and BWI76_RS21910 are separated by 5 nucleotides BWI76_RS21890: BWI76_RS21890 - oxidoreductase, at 4,574,091 to 4,575,167 _RS21890 BWI76_RS21895: BWI76_RS21895 - amino acid ABC transport system periplasmic amino acid-binding component, at 4,575,177 to 4,575,980 _RS21895 BWI76_RS21900: BWI76_RS21900 - amino acid ABC transporter permease/ATP-binding protein, at 4,576,005 to 4,577,525 _RS21900 BWI76_RS21905: BWI76_RS21905 - class II aldolase, at 4,577,526 to 4,578,254 _RS21905 BWI76_RS21910: BWI76_RS21910 - glyoxylate/hydroxypyruvate reductase A, at 4,578,260 to 4,579,192 _RS21910 Position (kb) 4576 4577 4578Strain fitness (log2 ratio) -3 -2 -1 0 1at 4575.036 kb on + strand, within BWI76_RS21890at 4575.044 kb on - strand, within BWI76_RS21890at 4575.068 kb on + strandat 4575.221 kb on - strandat 4575.221 kb on - strandat 4575.272 kb on + strand, within BWI76_RS21895at 4575.357 kb on + strand, within BWI76_RS21895at 4575.520 kb on + strand, within BWI76_RS21895at 4575.520 kb on + strand, within BWI76_RS21895at 4575.526 kb on - strand, within BWI76_RS21895at 4575.527 kb on - strand, within BWI76_RS21895at 4575.573 kb on - strand, within BWI76_RS21895at 4575.606 kb on - strand, within BWI76_RS21895at 4575.657 kb on + strand, within BWI76_RS21895at 4575.818 kb on - strand, within BWI76_RS21895at 4575.885 kb on - strand, within BWI76_RS21895at 4575.924 kb on - strandat 4575.925 kb on - strandat 4576.142 kb on + strandat 4576.143 kb on - strandat 4576.144 kb on + strandat 4576.159 kb on + strand, within BWI76_RS21900at 4576.250 kb on + strand, within BWI76_RS21900at 4576.261 kb on + strand, within BWI76_RS21900at 4576.327 kb on + strand, within BWI76_RS21900at 4576.400 kb on - strand, within BWI76_RS21900at 4576.400 kb on - strand, within BWI76_RS21900at 4576.400 kb on - strand, within BWI76_RS21900at 4576.550 kb on + strand, within BWI76_RS21900at 4576.584 kb on - strand, within BWI76_RS21900at 4576.648 kb on - strand, within BWI76_RS21900at 4576.648 kb on - strand, within BWI76_RS21900at 4576.690 kb on + strand, within BWI76_RS21900at 4576.905 kb on + strand, within BWI76_RS21900at 4577.012 kb on - strand, within BWI76_RS21900at 4577.076 kb on + strand, within BWI76_RS21900at 4577.115 kb on - strand, within BWI76_RS21900at 4577.449 kb on - strandat 4577.723 kb on + strand, within BWI76_RS21905at 4577.733 kb on + strand, within BWI76_RS21905at 4577.741 kb on - strand, within BWI76_RS21905at 4578.165 kb on - strand, within BWI76_RS21905at 4578.167 kb on + strand, within BWI76_RS21905at 4578.230 kb on + strandat 4578.296 kb on + strandat 4578.304 kb on - strandat 4578.342 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Histidine (N)
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4,575,036 + BWI76_RS21890 0.88 +0.8
4,575,044 - BWI76_RS21890 0.88 -1.4
4,575,068 + +1.0
4,575,221 - -1.6
4,575,221 - -1.8
4,575,272 + BWI76_RS21895 0.12 -1.2
4,575,357 + BWI76_RS21895 0.22 +0.1
4,575,520 + BWI76_RS21895 0.43 +1.4
4,575,520 + BWI76_RS21895 0.43 -0.2
4,575,526 - BWI76_RS21895 0.43 -1.8
4,575,527 - BWI76_RS21895 0.44 -1.4
4,575,573 - BWI76_RS21895 0.49 -0.9
4,575,606 - BWI76_RS21895 0.53 -3.0
4,575,657 + BWI76_RS21895 0.60 -0.7
4,575,818 - BWI76_RS21895 0.80 -2.1
4,575,885 - BWI76_RS21895 0.88 -3.5
4,575,924 - -2.5
4,575,925 - -3.2
4,576,142 + -2.9
4,576,143 - -1.4
4,576,144 + -2.2
4,576,159 + BWI76_RS21900 0.10 -0.5
4,576,250 + BWI76_RS21900 0.16 -1.6
4,576,261 + BWI76_RS21900 0.17 -1.6
4,576,327 + BWI76_RS21900 0.21 -1.8
4,576,400 - BWI76_RS21900 0.26 -1.9
4,576,400 - BWI76_RS21900 0.26 -2.4
4,576,400 - BWI76_RS21900 0.26 -3.0
4,576,550 + BWI76_RS21900 0.36 -1.3
4,576,584 - BWI76_RS21900 0.38 -3.7
4,576,648 - BWI76_RS21900 0.42 -0.3
4,576,648 - BWI76_RS21900 0.42 -1.7
4,576,690 + BWI76_RS21900 0.45 -1.1
4,576,905 + BWI76_RS21900 0.59 +0.4
4,577,012 - BWI76_RS21900 0.66 -2.8
4,577,076 + BWI76_RS21900 0.70 +0.5
4,577,115 - BWI76_RS21900 0.73 -1.1
4,577,449 - -0.5
4,577,723 + BWI76_RS21905 0.27 -1.8
4,577,733 + BWI76_RS21905 0.28 +0.6
4,577,741 - BWI76_RS21905 0.29 -1.9
4,578,165 - BWI76_RS21905 0.88 -3.7
4,578,167 + BWI76_RS21905 0.88 -2.3
4,578,230 + -0.8
4,578,296 + -2.9
4,578,304 - -2.9
4,578,342 - -2.5

Or see this region's nucleotide sequence