Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08000 and BWI76_RS08005 are separated by 186 nucleotides BWI76_RS08005 and BWI76_RS08010 are separated by 137 nucleotides BWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotides
BWI76_RS08000: BWI76_RS08000 - lipoyl synthase, at 1,696,223 to 1,697,188
_RS08000
BWI76_RS08005: BWI76_RS08005 - transcriptional regulator, at 1,697,375 to 1,698,328
_RS08005
BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110
_RS08010
BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465
_RS08015
Position (kb) 1697
1698
1699 Strain fitness (log2 ratio) -1
0
1 at 1697.308 kb on - strand at 1697.575 kb on - strand, within BWI76_RS08005 at 1697.970 kb on + strand, within BWI76_RS08005 at 1698.083 kb on + strand, within BWI76_RS08005 at 1698.091 kb on - strand, within BWI76_RS08005 at 1698.140 kb on + strand, within BWI76_RS08005 at 1698.211 kb on - strand, within BWI76_RS08005 at 1698.357 kb on + strand at 1698.357 kb on - strand at 1698.365 kb on - strand at 1698.459 kb on - strand at 1699.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 1,697,308 - +0.6 1,697,575 - BWI76_RS08005 0.21 +1.0 1,697,970 + BWI76_RS08005 0.62 +0.1 1,698,083 + BWI76_RS08005 0.74 +0.4 1,698,091 - BWI76_RS08005 0.75 +0.4 1,698,140 + BWI76_RS08005 0.80 -0.0 1,698,211 - BWI76_RS08005 0.88 +0.8 1,698,357 + -0.5 1,698,357 - -0.3 1,698,365 - +0.9 1,698,459 - -0.4 1,699,151 + -0.5
Or see this region's nucleotide sequence