Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5262 and Pf6N2E2_5263 are separated by 121 nucleotides Pf6N2E2_5263 and Pf6N2E2_5264 are separated by 215 nucleotides Pf6N2E2_5264 and Pf6N2E2_5265 overlap by 33 nucleotides Pf6N2E2_5265 and Pf6N2E2_5266 are separated by 137 nucleotides
Pf6N2E2_5262 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 6,057,501 to 6,057,986
_5262
Pf6N2E2_5263 - Glutathione peroxidase family protein, at 6,058,108 to 6,058,590
_5263
Pf6N2E2_5264 - hypothetical protein, at 6,058,806 to 6,058,940
_5264
Pf6N2E2_5265 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 6,058,908 to 6,060,011
_5265
Pf6N2E2_5266 - Glycosyltransferase, at 6,060,149 to 6,061,348
_5266
Position (kb)
6058
6059
6060
6061 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6057.997 kb on - strand at 6058.044 kb on + strand at 6058.058 kb on + strand at 6058.058 kb on + strand at 6058.059 kb on - strand at 6058.059 kb on - strand at 6058.128 kb on + strand at 6058.128 kb on + strand at 6058.129 kb on - strand at 6058.232 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.470 kb on + strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.672 kb on + strand at 6058.690 kb on + strand at 6058.807 kb on + strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.815 kb on - strand at 6058.830 kb on + strand, within Pf6N2E2_5264 at 6058.837 kb on - strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.863 kb on - strand, within Pf6N2E2_5264 at 6058.915 kb on + strand at 6058.916 kb on - strand at 6058.916 kb on - strand at 6059.034 kb on + strand, within Pf6N2E2_5265 at 6059.034 kb on + strand, within Pf6N2E2_5265 at 6059.035 kb on - strand, within Pf6N2E2_5265 at 6059.035 kb on - strand, within Pf6N2E2_5265 at 6059.038 kb on - strand, within Pf6N2E2_5265 at 6059.136 kb on + strand, within Pf6N2E2_5265 at 6059.136 kb on + strand, within Pf6N2E2_5265 at 6059.137 kb on - strand, within Pf6N2E2_5265 at 6059.183 kb on - strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.673 kb on - strand, within Pf6N2E2_5265 at 6060.009 kb on + strand at 6060.010 kb on - strand at 6060.031 kb on + strand at 6060.032 kb on - strand at 6060.032 kb on - strand at 6060.058 kb on + strand at 6060.058 kb on + strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.367 kb on - strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.982 kb on + strand, within Pf6N2E2_5266
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 6,057,997 - +0.2 6,058,044 + -1.7 6,058,058 + -0.3 6,058,058 + -1.1 6,058,059 - +1.6 6,058,059 - -1.4 6,058,128 + -1.9 6,058,128 + +0.2 6,058,129 - -0.4 6,058,232 - Pf6N2E2_5263 0.26 +0.2 6,058,253 - Pf6N2E2_5263 0.30 +0.3 6,058,253 - Pf6N2E2_5263 0.30 -0.1 6,058,253 - Pf6N2E2_5263 0.30 -1.2 6,058,261 + Pf6N2E2_5263 0.32 -0.4 6,058,261 + Pf6N2E2_5263 0.32 -0.2 6,058,261 + Pf6N2E2_5263 0.32 +0.2 6,058,262 - Pf6N2E2_5263 0.32 +0.7 6,058,262 - Pf6N2E2_5263 0.32 -0.6 6,058,262 - Pf6N2E2_5263 0.32 +0.1 6,058,262 - Pf6N2E2_5263 0.32 -1.2 6,058,262 - Pf6N2E2_5263 0.32 +0.0 6,058,262 - Pf6N2E2_5263 0.32 +0.8 6,058,262 - Pf6N2E2_5263 0.32 +1.2 6,058,262 - Pf6N2E2_5263 0.32 +1.6 6,058,470 + Pf6N2E2_5263 0.75 -0.3 6,058,471 - Pf6N2E2_5263 0.75 +2.0 6,058,471 - Pf6N2E2_5263 0.75 +0.1 6,058,672 + -0.8 6,058,690 + +1.0 6,058,807 + -0.4 6,058,808 - -0.8 6,058,808 - +0.7 6,058,808 - +1.1 6,058,815 - -0.4 6,058,830 + Pf6N2E2_5264 0.18 +0.3 6,058,837 - Pf6N2E2_5264 0.23 -1.4 6,058,859 + Pf6N2E2_5264 0.39 -1.0 6,058,859 + Pf6N2E2_5264 0.39 +0.1 6,058,859 + Pf6N2E2_5264 0.39 -0.5 6,058,859 + Pf6N2E2_5264 0.39 -0.4 6,058,860 - Pf6N2E2_5264 0.40 +0.1 6,058,860 - Pf6N2E2_5264 0.40 +0.6 6,058,860 - Pf6N2E2_5264 0.40 -0.2 6,058,863 - Pf6N2E2_5264 0.42 -0.1 6,058,915 + +1.3 6,058,916 - -0.2 6,058,916 - +0.6 6,059,034 + Pf6N2E2_5265 0.11 -0.3 6,059,034 + Pf6N2E2_5265 0.11 -0.0 6,059,035 - Pf6N2E2_5265 0.12 +0.2 6,059,035 - Pf6N2E2_5265 0.12 -0.4 6,059,038 - Pf6N2E2_5265 0.12 -1.5 6,059,136 + Pf6N2E2_5265 0.21 +0.0 6,059,136 + Pf6N2E2_5265 0.21 +0.1 6,059,137 - Pf6N2E2_5265 0.21 -0.4 6,059,183 - Pf6N2E2_5265 0.25 -2.4 6,059,598 + Pf6N2E2_5265 0.62 +0.7 6,059,598 + Pf6N2E2_5265 0.62 -0.4 6,059,673 - Pf6N2E2_5265 0.69 -1.0 6,060,009 + -0.9 6,060,010 - -0.4 6,060,031 + +1.0 6,060,032 - -0.1 6,060,032 - +0.5 6,060,058 + +1.3 6,060,058 + -0.0 6,060,059 - +0.1 6,060,059 - -1.5 6,060,059 - -0.8 6,060,059 - +0.2 6,060,059 - +0.9 6,060,366 + Pf6N2E2_5266 0.18 +0.5 6,060,366 + Pf6N2E2_5266 0.18 -0.7 6,060,366 + Pf6N2E2_5266 0.18 +1.4 6,060,367 - Pf6N2E2_5266 0.18 +1.2 6,060,405 + Pf6N2E2_5266 0.21 -0.3 6,060,405 + Pf6N2E2_5266 0.21 +0.4 6,060,406 - Pf6N2E2_5266 0.21 -0.7 6,060,406 - Pf6N2E2_5266 0.21 -0.8 6,060,501 + Pf6N2E2_5266 0.29 +0.0 6,060,501 + Pf6N2E2_5266 0.29 -1.6 6,060,894 + Pf6N2E2_5266 0.62 -0.6 6,060,894 + Pf6N2E2_5266 0.62 +0.9 6,060,895 - Pf6N2E2_5266 0.62 +0.3 6,060,895 - Pf6N2E2_5266 0.62 -0.1 6,060,895 - Pf6N2E2_5266 0.62 +0.6 6,060,906 - Pf6N2E2_5266 0.63 -0.2 6,060,906 - Pf6N2E2_5266 0.63 -0.6 6,060,942 + Pf6N2E2_5266 0.66 -0.3 6,060,942 + Pf6N2E2_5266 0.66 +0.9 6,060,943 - Pf6N2E2_5266 0.66 +1.2 6,060,943 - Pf6N2E2_5266 0.66 -0.4 6,060,943 - Pf6N2E2_5266 0.66 -0.0 6,060,982 + Pf6N2E2_5266 0.69 +0.3
Or see this region's nucleotide sequence