Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5000 and Pf6N2E2_5001 are separated by 168 nucleotides Pf6N2E2_5001 and Pf6N2E2_5002 are separated by 81 nucleotides Pf6N2E2_5002 and Pf6N2E2_5003 overlap by 1 nucleotides
Pf6N2E2_5000 - Rare lipoprotein A precursor, at 5,766,197 to 5,767,222
_5000
Pf6N2E2_5001 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 5,767,391 to 5,768,548
_5001
Pf6N2E2_5002 - Proposed lipoate regulatory protein YbeD, at 5,768,630 to 5,768,905
_5002
Pf6N2E2_5003 - Octanoate-[acyl-carrier-protein]-protein-N-octan oyltransferase, at 5,768,905 to 5,769,552
_5003
Position (kb)
5767
5768
5769 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5767.275 kb on + strand at 5767.275 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.312 kb on - strand at 5767.312 kb on - strand at 5767.602 kb on - strand, within Pf6N2E2_5001 at 5767.904 kb on + strand, within Pf6N2E2_5001 at 5768.231 kb on + strand, within Pf6N2E2_5001 at 5768.491 kb on + strand at 5768.531 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 5,767,275 + -0.6 5,767,275 + +1.1 5,767,311 + -1.0 5,767,311 + -0.4 5,767,311 + -1.3 5,767,312 - +0.8 5,767,312 - -0.2 5,767,602 - Pf6N2E2_5001 0.18 -0.5 5,767,904 + Pf6N2E2_5001 0.44 -2.7 5,768,231 + Pf6N2E2_5001 0.73 +0.1 5,768,491 + -0.2 5,768,531 + -2.8
Or see this region's nucleotide sequence