Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4717 and Pf6N2E2_4718 are separated by 21 nucleotides Pf6N2E2_4718 and Pf6N2E2_4719 are separated by 14 nucleotides Pf6N2E2_4719 and Pf6N2E2_4720 overlap by 4 nucleotides
Pf6N2E2_4717 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,453,920 to 5,455,173
_4717
Pf6N2E2_4718 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 5,455,195 to 5,456,445
_4718
Pf6N2E2_4719 - Sarcosine oxidase delta subunit (EC 1.5.3.1), at 5,456,460 to 5,456,789
_4719
Pf6N2E2_4720 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 5,456,786 to 5,459,803
_4720
Position (kb)
5455
5456
5457 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.236 kb on - strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.338 kb on - strand, within Pf6N2E2_4717 at 5454.352 kb on + strand, within Pf6N2E2_4717 at 5454.385 kb on + strand, within Pf6N2E2_4717 at 5454.452 kb on - strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5455.136 kb on + strand at 5455.187 kb on + strand at 5455.187 kb on + strand at 5455.188 kb on - strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.204 kb on + strand at 5455.269 kb on + strand at 5455.291 kb on + strand at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.340 kb on - strand, within Pf6N2E2_4718 at 5455.444 kb on + strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.471 kb on + strand, within Pf6N2E2_4718 at 5455.472 kb on - strand, within Pf6N2E2_4718 at 5455.520 kb on - strand, within Pf6N2E2_4718 at 5455.549 kb on + strand, within Pf6N2E2_4718 at 5455.622 kb on + strand, within Pf6N2E2_4718 at 5455.623 kb on - strand, within Pf6N2E2_4718 at 5456.041 kb on + strand, within Pf6N2E2_4718 at 5456.095 kb on + strand, within Pf6N2E2_4718 at 5456.443 kb on + strand at 5456.460 kb on - strand at 5456.460 kb on - strand at 5456.460 kb on - strand at 5456.586 kb on + strand, within Pf6N2E2_4719 at 5456.586 kb on + strand, within Pf6N2E2_4719 at 5456.586 kb on + strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.587 kb on - strand, within Pf6N2E2_4719 at 5456.658 kb on + strand, within Pf6N2E2_4719 at 5456.706 kb on + strand, within Pf6N2E2_4719 at 5456.785 kb on + strand at 5456.785 kb on + strand at 5456.785 kb on + strand at 5456.785 kb on + strand at 5456.786 kb on - strand at 5456.786 kb on - strand at 5456.786 kb on - strand at 5456.786 kb on - strand at 5456.867 kb on + strand at 5456.867 kb on + strand at 5456.868 kb on - strand at 5456.945 kb on + strand at 5457.167 kb on + strand, within Pf6N2E2_4720 at 5457.168 kb on - strand, within Pf6N2E2_4720 at 5457.168 kb on - strand, within Pf6N2E2_4720 at 5457.170 kb on + strand, within Pf6N2E2_4720 at 5457.171 kb on - strand, within Pf6N2E2_4720 at 5457.186 kb on - strand, within Pf6N2E2_4720 at 5457.209 kb on + strand, within Pf6N2E2_4720 at 5457.210 kb on - strand, within Pf6N2E2_4720 at 5457.218 kb on + strand, within Pf6N2E2_4720 at 5457.218 kb on + strand, within Pf6N2E2_4720 at 5457.219 kb on - strand, within Pf6N2E2_4720 at 5457.227 kb on - strand, within Pf6N2E2_4720 at 5457.275 kb on + strand, within Pf6N2E2_4720 at 5457.275 kb on + strand, within Pf6N2E2_4720 at 5457.275 kb on + strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.276 kb on - strand, within Pf6N2E2_4720 at 5457.281 kb on + strand, within Pf6N2E2_4720 at 5457.281 kb on + strand, within Pf6N2E2_4720 at 5457.281 kb on + strand, within Pf6N2E2_4720 at 5457.281 kb on + strand, within Pf6N2E2_4720 at 5457.281 kb on + strand, within Pf6N2E2_4720 at 5457.282 kb on - strand, within Pf6N2E2_4720 at 5457.282 kb on - strand, within Pf6N2E2_4720
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 5,454,233 + Pf6N2E2_4717 0.25 +0.9 5,454,233 + Pf6N2E2_4717 0.25 -1.2 5,454,234 - Pf6N2E2_4717 0.25 +0.2 5,454,234 - Pf6N2E2_4717 0.25 +0.4 5,454,236 - Pf6N2E2_4717 0.25 +0.0 5,454,337 + Pf6N2E2_4717 0.33 +0.4 5,454,337 + Pf6N2E2_4717 0.33 -0.7 5,454,338 - Pf6N2E2_4717 0.33 -0.1 5,454,352 + Pf6N2E2_4717 0.34 +0.5 5,454,385 + Pf6N2E2_4717 0.37 +1.4 5,454,452 - Pf6N2E2_4717 0.42 +0.2 5,454,845 + Pf6N2E2_4717 0.74 -0.9 5,454,845 + Pf6N2E2_4717 0.74 -2.3 5,454,845 + Pf6N2E2_4717 0.74 -0.7 5,454,845 + Pf6N2E2_4717 0.74 +0.6 5,454,846 - Pf6N2E2_4717 0.74 -0.3 5,454,846 - Pf6N2E2_4717 0.74 +0.3 5,454,846 - Pf6N2E2_4717 0.74 -0.7 5,454,846 - Pf6N2E2_4717 0.74 +0.6 5,454,846 - Pf6N2E2_4717 0.74 -1.0 5,455,136 + -0.3 5,455,187 + -0.1 5,455,187 + -1.1 5,455,188 - +0.0 5,455,194 + +0.4 5,455,194 + -1.7 5,455,194 + +0.5 5,455,194 + -0.8 5,455,195 - -2.6 5,455,195 - -1.9 5,455,195 - -0.0 5,455,204 + -2.0 5,455,269 + +0.8 5,455,291 + +0.1 5,455,339 + Pf6N2E2_4718 0.12 -0.6 5,455,339 + Pf6N2E2_4718 0.12 +0.5 5,455,339 + Pf6N2E2_4718 0.12 +0.3 5,455,340 - Pf6N2E2_4718 0.12 -1.2 5,455,444 + Pf6N2E2_4718 0.20 -1.2 5,455,445 - Pf6N2E2_4718 0.20 -0.3 5,455,445 - Pf6N2E2_4718 0.20 -0.3 5,455,445 - Pf6N2E2_4718 0.20 -0.2 5,455,471 + Pf6N2E2_4718 0.22 -0.7 5,455,472 - Pf6N2E2_4718 0.22 -2.7 5,455,520 - Pf6N2E2_4718 0.26 -1.1 5,455,549 + Pf6N2E2_4718 0.28 -0.9 5,455,622 + Pf6N2E2_4718 0.34 +0.8 5,455,623 - Pf6N2E2_4718 0.34 -0.3 5,456,041 + Pf6N2E2_4718 0.68 +0.1 5,456,095 + Pf6N2E2_4718 0.72 -0.5 5,456,443 + +0.2 5,456,460 - -0.7 5,456,460 - +0.5 5,456,460 - +0.4 5,456,586 + Pf6N2E2_4719 0.38 +0.2 5,456,586 + Pf6N2E2_4719 0.38 -1.5 5,456,586 + Pf6N2E2_4719 0.38 +1.8 5,456,587 - Pf6N2E2_4719 0.38 +0.8 5,456,587 - Pf6N2E2_4719 0.38 +0.0 5,456,587 - Pf6N2E2_4719 0.38 -0.2 5,456,587 - Pf6N2E2_4719 0.38 +3.2 5,456,587 - Pf6N2E2_4719 0.38 -1.2 5,456,587 - Pf6N2E2_4719 0.38 +0.5 5,456,587 - Pf6N2E2_4719 0.38 -1.6 5,456,587 - Pf6N2E2_4719 0.38 +3.2 5,456,587 - Pf6N2E2_4719 0.38 -0.3 5,456,658 + Pf6N2E2_4719 0.60 -2.1 5,456,706 + Pf6N2E2_4719 0.75 +0.5 5,456,785 + -0.9 5,456,785 + -0.3 5,456,785 + +0.3 5,456,785 + +0.7 5,456,786 - +0.2 5,456,786 - -0.9 5,456,786 - +0.0 5,456,786 - -0.0 5,456,867 + -1.0 5,456,867 + -0.1 5,456,868 - -0.6 5,456,945 + -0.0 5,457,167 + Pf6N2E2_4720 0.13 +0.3 5,457,168 - Pf6N2E2_4720 0.13 +0.4 5,457,168 - Pf6N2E2_4720 0.13 -0.6 5,457,170 + Pf6N2E2_4720 0.13 +0.9 5,457,171 - Pf6N2E2_4720 0.13 +0.4 5,457,186 - Pf6N2E2_4720 0.13 +0.2 5,457,209 + Pf6N2E2_4720 0.14 -0.4 5,457,210 - Pf6N2E2_4720 0.14 +0.1 5,457,218 + Pf6N2E2_4720 0.14 -2.2 5,457,218 + Pf6N2E2_4720 0.14 -1.3 5,457,219 - Pf6N2E2_4720 0.14 +0.8 5,457,227 - Pf6N2E2_4720 0.15 +0.1 5,457,275 + Pf6N2E2_4720 0.16 +0.8 5,457,275 + Pf6N2E2_4720 0.16 -1.0 5,457,275 + Pf6N2E2_4720 0.16 -0.7 5,457,276 - Pf6N2E2_4720 0.16 -0.7 5,457,276 - Pf6N2E2_4720 0.16 +1.4 5,457,276 - Pf6N2E2_4720 0.16 +0.4 5,457,276 - Pf6N2E2_4720 0.16 +0.6 5,457,276 - Pf6N2E2_4720 0.16 +4.3 5,457,276 - Pf6N2E2_4720 0.16 +0.4 5,457,281 + Pf6N2E2_4720 0.16 +0.2 5,457,281 + Pf6N2E2_4720 0.16 -0.6 5,457,281 + Pf6N2E2_4720 0.16 +0.4 5,457,281 + Pf6N2E2_4720 0.16 +0.2 5,457,281 + Pf6N2E2_4720 0.16 -0.0 5,457,282 - Pf6N2E2_4720 0.16 +0.1 5,457,282 - Pf6N2E2_4720 0.16 +0.2
Or see this region's nucleotide sequence