Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_289 and Pf6N2E2_290 are separated by 77 nucleotides Pf6N2E2_290 and Pf6N2E2_291 are separated by 343 nucleotides Pf6N2E2_291 and Pf6N2E2_292 are separated by 29 nucleotides Pf6N2E2_292 and Pf6N2E2_293 are separated by 217 nucleotides Pf6N2E2_293 and Pf6N2E2_294 are separated by 123 nucleotides
Pf6N2E2_289 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 358,365 to 359,621
_289
Pf6N2E2_290 - Cold shock protein CspD, at 359,699 to 359,968
_290
Pf6N2E2_291 - ATP-dependent Clp protease adaptor protein ClpS, at 360,312 to 360,557
_291
Pf6N2E2_292 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 360,587 to 362,857
_292
Pf6N2E2_293 - Translation initiation factor 1, at 363,075 to 363,293
_293
Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124
_294
Position (kb) 360
361
362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 359.619 kb on + strand at 359.619 kb on + strand at 359.619 kb on + strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.836 kb on + strand, within Pf6N2E2_290 at 359.836 kb on + strand, within Pf6N2E2_290 at 359.837 kb on - strand, within Pf6N2E2_290 at 359.837 kb on - strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.839 kb on - strand, within Pf6N2E2_290 at 359.862 kb on + strand, within Pf6N2E2_290 at 359.968 kb on + strand at 359.968 kb on + strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.029 kb on + strand at 360.030 kb on - strand at 360.040 kb on + strand at 360.040 kb on + strand at 360.040 kb on + strand at 360.041 kb on - strand at 360.041 kb on - strand at 360.041 kb on - strand at 360.047 kb on - strand at 360.083 kb on - strand at 360.083 kb on - strand at 360.092 kb on - strand at 360.092 kb on - strand at 360.165 kb on + strand at 360.165 kb on + strand at 360.176 kb on + strand at 360.176 kb on + strand at 360.177 kb on - strand at 360.177 kb on - strand at 360.221 kb on - strand at 360.221 kb on - strand at 360.248 kb on - strand at 360.298 kb on + strand at 360.298 kb on + strand at 360.299 kb on - strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.316 kb on - strand at 360.342 kb on + strand, within Pf6N2E2_291 at 360.342 kb on + strand, within Pf6N2E2_291 at 360.342 kb on + strand, within Pf6N2E2_291 at 360.431 kb on + strand, within Pf6N2E2_291 at 360.431 kb on + strand, within Pf6N2E2_291 at 360.432 kb on - strand, within Pf6N2E2_291 at 360.448 kb on + strand, within Pf6N2E2_291 at 360.448 kb on + strand, within Pf6N2E2_291 at 360.449 kb on - strand, within Pf6N2E2_291 at 360.530 kb on - strand, within Pf6N2E2_291 at 360.573 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.587 kb on - strand at 360.587 kb on - strand at 360.587 kb on - strand at 360.591 kb on + strand at 360.688 kb on - strand at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.995 kb on + strand, within Pf6N2E2_292 at 360.996 kb on - strand, within Pf6N2E2_292 at 361.058 kb on - strand, within Pf6N2E2_292 at 361.113 kb on - strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.335 kb on - strand, within Pf6N2E2_292 at 361.666 kb on + strand, within Pf6N2E2_292 at 361.760 kb on + strand, within Pf6N2E2_292 at 361.761 kb on - strand, within Pf6N2E2_292 at 361.761 kb on - strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.169 kb on - strand, within Pf6N2E2_292 at 362.169 kb on - strand, within Pf6N2E2_292 at 362.216 kb on + strand, within Pf6N2E2_292 at 362.216 kb on + strand, within Pf6N2E2_292 at 362.217 kb on - strand, within Pf6N2E2_292 at 362.217 kb on - strand, within Pf6N2E2_292 at 362.277 kb on + strand, within Pf6N2E2_292 at 362.278 kb on - strand, within Pf6N2E2_292 at 362.907 kb on - strand at 362.907 kb on - strand at 363.396 kb on - strand at 363.396 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.404 kb on - strand at 363.479 kb on - strand at 363.517 kb on + strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.617 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.788 kb on - strand, within Pf6N2E2_294 at 363.797 kb on - strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 359,619 + +0.1 359,619 + +0.3 359,619 + +0.3 359,620 - -0.5 359,620 - +0.9 359,620 - -1.4 359,620 - +0.2 359,620 - -2.1 359,620 - -0.2 359,836 + Pf6N2E2_290 0.51 -0.5 359,836 + Pf6N2E2_290 0.51 -0.1 359,837 - Pf6N2E2_290 0.51 +0.5 359,837 - Pf6N2E2_290 0.51 -0.2 359,838 + Pf6N2E2_290 0.51 -0.4 359,838 + Pf6N2E2_290 0.51 +1.5 359,838 + Pf6N2E2_290 0.51 -0.5 359,839 - Pf6N2E2_290 0.52 +0.7 359,862 + Pf6N2E2_290 0.60 -0.2 359,968 + +2.2 359,968 + +0.1 360,007 - -2.2 360,007 - -2.9 360,007 - -0.1 360,007 - -1.2 360,029 + +0.3 360,030 - -0.8 360,040 + +0.3 360,040 + -0.2 360,040 + -1.7 360,041 - -1.2 360,041 - -2.6 360,041 - -0.2 360,047 - -2.0 360,083 - -1.1 360,083 - -1.6 360,092 - -2.5 360,092 - -3.1 360,165 + +1.5 360,165 + +0.6 360,176 + +0.2 360,176 + -0.1 360,177 - -0.7 360,177 - -1.8 360,221 - -1.3 360,221 - -1.7 360,248 - -1.5 360,298 + -1.1 360,298 + -1.1 360,299 - +0.7 360,315 + +1.4 360,315 + +1.0 360,315 + -0.6 360,315 + +0.4 360,316 - -3.1 360,342 + Pf6N2E2_291 0.12 +0.8 360,342 + Pf6N2E2_291 0.12 -1.1 360,342 + Pf6N2E2_291 0.12 +2.3 360,431 + Pf6N2E2_291 0.48 -1.9 360,431 + Pf6N2E2_291 0.48 +0.8 360,432 - Pf6N2E2_291 0.49 -0.6 360,448 + Pf6N2E2_291 0.55 -0.8 360,448 + Pf6N2E2_291 0.55 +0.2 360,449 - Pf6N2E2_291 0.56 -0.1 360,530 - Pf6N2E2_291 0.89 -2.4 360,573 + +1.4 360,586 + -1.5 360,586 + -2.6 360,586 + -2.3 360,586 + -2.6 360,587 - -1.1 360,587 - -2.7 360,587 - -1.1 360,591 + +0.4 360,688 - +0.8 360,870 + Pf6N2E2_292 0.12 -2.2 360,870 + Pf6N2E2_292 0.12 -1.9 360,870 + Pf6N2E2_292 0.12 -0.1 360,870 + Pf6N2E2_292 0.12 -1.5 360,870 + Pf6N2E2_292 0.12 +0.1 360,871 - Pf6N2E2_292 0.13 -1.2 360,871 - Pf6N2E2_292 0.13 -0.7 360,871 - Pf6N2E2_292 0.13 -0.3 360,871 - Pf6N2E2_292 0.13 -0.5 360,995 + Pf6N2E2_292 0.18 -1.1 360,996 - Pf6N2E2_292 0.18 -1.9 361,058 - Pf6N2E2_292 0.21 +0.0 361,113 - Pf6N2E2_292 0.23 -1.3 361,334 + Pf6N2E2_292 0.33 -2.6 361,334 + Pf6N2E2_292 0.33 -0.8 361,334 + Pf6N2E2_292 0.33 -0.1 361,335 - Pf6N2E2_292 0.33 -0.5 361,666 + Pf6N2E2_292 0.48 -2.0 361,760 + Pf6N2E2_292 0.52 -0.3 361,761 - Pf6N2E2_292 0.52 -1.5 361,761 - Pf6N2E2_292 0.52 -1.8 362,168 + Pf6N2E2_292 0.70 -1.3 362,168 + Pf6N2E2_292 0.70 -1.3 362,168 + Pf6N2E2_292 0.70 -2.1 362,168 + Pf6N2E2_292 0.70 -3.1 362,169 - Pf6N2E2_292 0.70 -1.3 362,169 - Pf6N2E2_292 0.70 -0.6 362,216 + Pf6N2E2_292 0.72 -2.2 362,216 + Pf6N2E2_292 0.72 -0.8 362,217 - Pf6N2E2_292 0.72 -2.1 362,217 - Pf6N2E2_292 0.72 -1.3 362,277 + Pf6N2E2_292 0.74 -1.8 362,278 - Pf6N2E2_292 0.74 -2.2 362,907 - +0.2 362,907 - -1.5 363,396 - +0.2 363,396 - +0.8 363,402 - +0.8 363,402 - -0.1 363,402 - -0.4 363,404 - -0.4 363,479 - +0.8 363,517 + Pf6N2E2_294 0.14 -0.1 363,518 - Pf6N2E2_294 0.14 +1.8 363,518 - Pf6N2E2_294 0.14 +0.6 363,608 - Pf6N2E2_294 0.27 +0.8 363,608 - Pf6N2E2_294 0.27 +0.2 363,608 - Pf6N2E2_294 0.27 -0.9 363,616 + Pf6N2E2_294 0.28 +1.4 363,616 + Pf6N2E2_294 0.28 +1.8 363,616 + Pf6N2E2_294 0.28 +1.9 363,617 - Pf6N2E2_294 0.28 -1.1 363,692 - Pf6N2E2_294 0.39 +0.1 363,692 - Pf6N2E2_294 0.39 +0.7 363,754 + Pf6N2E2_294 0.48 -0.8 363,754 + Pf6N2E2_294 0.48 +0.8 363,754 + Pf6N2E2_294 0.48 +0.5 363,755 - Pf6N2E2_294 0.48 +0.4 363,755 - Pf6N2E2_294 0.48 +0.4 363,787 + Pf6N2E2_294 0.52 -0.4 363,787 + Pf6N2E2_294 0.52 +0.6 363,787 + Pf6N2E2_294 0.52 +1.5 363,787 + Pf6N2E2_294 0.52 +0.1 363,788 - Pf6N2E2_294 0.52 -0.7 363,797 - Pf6N2E2_294 0.54 +0.3 363,808 + Pf6N2E2_294 0.55 +1.2 363,808 + Pf6N2E2_294 0.55 +0.2 363,809 - Pf6N2E2_294 0.55 +1.8 363,809 - Pf6N2E2_294 0.55 +0.9
Or see this region's nucleotide sequence