Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_281 and Pf6N2E2_282 are separated by 170 nucleotides Pf6N2E2_282 and Pf6N2E2_283 overlap by 8 nucleotides
Pf6N2E2_281 - Type II and III secretion system family protein, at 347,655 to 348,416
_281
Pf6N2E2_282 - GNAT family acetyltransferase YjcF, at 348,587 to 349,012
_282
Pf6N2E2_283 - FIG002776: hypothetical protein, at 349,005 to 350,171
_283
Position (kb) 348
349
350 Strain fitness (log2 ratio) -2
-1
0
1 at 347.611 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.714 kb on - strand at 347.826 kb on + strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand at 347.911 kb on + strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 348.165 kb on - strand, within Pf6N2E2_281 at 348.446 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.672 kb on + strand, within Pf6N2E2_282 at 348.672 kb on + strand, within Pf6N2E2_282 at 349.006 kb on + strand at 349.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 347,611 + +0.2 347,713 + -0.3 347,713 + +0.3 347,713 + -1.4 347,714 - -0.1 347,826 + Pf6N2E2_281 0.22 -0.7 347,827 - Pf6N2E2_281 0.23 -0.4 347,827 - Pf6N2E2_281 0.23 +1.1 347,827 - -0.5 347,911 + Pf6N2E2_281 0.34 -0.1 347,912 - Pf6N2E2_281 0.34 -0.3 347,912 - Pf6N2E2_281 0.34 -0.4 347,962 + Pf6N2E2_281 0.40 -0.1 347,962 + Pf6N2E2_281 0.40 -0.5 347,962 + Pf6N2E2_281 0.40 -0.3 347,962 + Pf6N2E2_281 0.40 -0.8 347,963 - Pf6N2E2_281 0.40 -2.1 347,963 - Pf6N2E2_281 0.40 +0.6 347,963 - Pf6N2E2_281 0.40 +0.2 348,165 - Pf6N2E2_281 0.67 +0.5 348,446 + +0.1 348,487 + -0.3 348,487 + +1.2 348,487 + -0.1 348,488 - -0.4 348,488 - +0.8 348,488 - -0.3 348,672 + Pf6N2E2_282 0.20 -2.3 348,672 + Pf6N2E2_282 0.20 -1.6 349,006 + +0.2 349,007 - -1.1
Or see this region's nucleotide sequence