Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1072 and Pf6N2E2_1073 are separated by 250 nucleotides Pf6N2E2_1073 and Pf6N2E2_1074 are separated by 229 nucleotides Pf6N2E2_1074 and Pf6N2E2_1075 are separated by 20 nucleotides
Pf6N2E2_1072 - LmbE-like protein, at 1,250,862 to 1,251,626
_1072
Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036
_1073
Pf6N2E2_1074 - FIG00953356: hypothetical protein, at 1,254,266 to 1,254,619
_1074
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Position (kb)
1251
1252
1253
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.646 kb on - strand at 1251.674 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.864 kb on + strand at 1251.874 kb on - strand at 1251.936 kb on - strand at 1252.072 kb on + strand at 1252.073 kb on - strand at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.389 kb on + strand, within Pf6N2E2_1073 at 1252.441 kb on - strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073 at 1252.667 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.084 kb on + strand, within Pf6N2E2_1073 at 1253.084 kb on + strand, within Pf6N2E2_1073 at 1253.114 kb on + strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.270 kb on + strand, within Pf6N2E2_1073 at 1253.271 kb on - strand, within Pf6N2E2_1073 at 1253.306 kb on + strand, within Pf6N2E2_1073 at 1253.306 kb on + strand, within Pf6N2E2_1073 at 1253.307 kb on - strand, within Pf6N2E2_1073 at 1253.315 kb on + strand, within Pf6N2E2_1073 at 1253.315 kb on + strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.446 kb on - strand at 1253.507 kb on + strand, within Pf6N2E2_1073 at 1253.507 kb on + strand, within Pf6N2E2_1073 at 1253.765 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.819 kb on + strand, within Pf6N2E2_1073 at 1253.834 kb on + strand at 1253.834 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.037 kb on - strand at 1254.037 kb on - strand at 1254.132 kb on + strand at 1254.133 kb on - strand at 1254.133 kb on - strand at 1254.560 kb on - strand, within Pf6N2E2_1074 at 1254.583 kb on - strand, within Pf6N2E2_1074 at 1254.850 kb on + strand at 1254.850 kb on + strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.956 kb on - strand, within Pf6N2E2_1075
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 1,250,965 + Pf6N2E2_1072 0.13 -1.2 1,250,965 + Pf6N2E2_1072 0.13 -0.2 1,250,965 + Pf6N2E2_1072 0.13 +1.3 1,250,966 - Pf6N2E2_1072 0.14 -0.6 1,250,966 - Pf6N2E2_1072 0.14 -0.7 1,250,966 - Pf6N2E2_1072 0.14 -0.1 1,250,971 + Pf6N2E2_1072 0.14 +0.3 1,250,971 + Pf6N2E2_1072 0.14 -0.4 1,250,971 + Pf6N2E2_1072 0.14 +0.2 1,250,972 - Pf6N2E2_1072 0.14 -0.4 1,250,972 - Pf6N2E2_1072 0.14 -0.3 1,250,972 - Pf6N2E2_1072 0.14 -1.3 1,251,351 - Pf6N2E2_1072 0.64 +0.9 1,251,351 - Pf6N2E2_1072 0.64 +1.6 1,251,351 - Pf6N2E2_1072 0.64 -1.3 1,251,351 - Pf6N2E2_1072 0.64 +1.5 1,251,646 - -0.0 1,251,674 + +0.2 1,251,750 + +0.4 1,251,750 + +0.6 1,251,750 + +0.2 1,251,750 + +0.8 1,251,751 - +0.2 1,251,751 - +0.5 1,251,751 - -0.4 1,251,864 + +1.7 1,251,874 - -0.4 1,251,936 - -0.9 1,252,072 + -2.1 1,252,073 - -0.1 1,252,238 + Pf6N2E2_1073 0.17 +0.2 1,252,238 + Pf6N2E2_1073 0.17 -1.6 1,252,238 + Pf6N2E2_1073 0.17 +0.7 1,252,238 + Pf6N2E2_1073 0.17 +0.5 1,252,238 + Pf6N2E2_1073 0.17 -0.7 1,252,239 - Pf6N2E2_1073 0.17 -1.4 1,252,239 - Pf6N2E2_1073 0.17 -0.1 1,252,239 - Pf6N2E2_1073 0.17 +0.1 1,252,239 - Pf6N2E2_1073 0.17 +0.2 1,252,239 - Pf6N2E2_1073 0.17 -1.3 1,252,239 - Pf6N2E2_1073 0.17 -0.6 1,252,239 - Pf6N2E2_1073 0.17 -0.8 1,252,239 - Pf6N2E2_1073 0.17 -1.4 1,252,239 - Pf6N2E2_1073 0.17 -3.1 1,252,239 - Pf6N2E2_1073 0.17 -0.5 1,252,239 - Pf6N2E2_1073 0.17 +0.2 1,252,239 - Pf6N2E2_1073 0.17 -0.6 1,252,239 - Pf6N2E2_1073 0.17 +0.5 1,252,379 + Pf6N2E2_1073 0.23 -0.7 1,252,379 + Pf6N2E2_1073 0.23 -1.8 1,252,380 - Pf6N2E2_1073 0.23 +1.7 1,252,380 - Pf6N2E2_1073 0.23 -1.8 1,252,389 + Pf6N2E2_1073 0.24 +0.3 1,252,441 - Pf6N2E2_1073 0.26 -0.5 1,252,607 + Pf6N2E2_1073 0.34 -2.0 1,252,607 + Pf6N2E2_1073 0.34 -0.1 1,252,608 - Pf6N2E2_1073 0.34 +1.0 1,252,608 - Pf6N2E2_1073 0.34 -3.0 1,252,667 + Pf6N2E2_1073 0.37 -1.5 1,253,081 + Pf6N2E2_1073 0.56 -0.5 1,253,081 + Pf6N2E2_1073 0.56 -0.6 1,253,081 + Pf6N2E2_1073 0.56 +0.1 1,253,081 + Pf6N2E2_1073 0.56 -0.6 1,253,081 + Pf6N2E2_1073 0.56 -1.1 1,253,081 + Pf6N2E2_1073 0.56 -0.8 1,253,081 + Pf6N2E2_1073 0.56 +0.2 1,253,081 + Pf6N2E2_1073 0.56 -0.0 1,253,081 + Pf6N2E2_1073 0.56 -2.1 1,253,082 - Pf6N2E2_1073 0.56 -0.6 1,253,082 - Pf6N2E2_1073 0.56 -1.6 1,253,082 - Pf6N2E2_1073 0.56 -1.4 1,253,082 - Pf6N2E2_1073 0.56 -1.6 1,253,082 - Pf6N2E2_1073 0.56 +0.2 1,253,082 - Pf6N2E2_1073 0.56 -3.0 1,253,082 - Pf6N2E2_1073 0.56 -0.5 1,253,084 + Pf6N2E2_1073 0.56 +0.5 1,253,084 + Pf6N2E2_1073 0.56 -0.3 1,253,114 + Pf6N2E2_1073 0.57 -0.3 1,253,115 - Pf6N2E2_1073 0.57 +0.2 1,253,115 - Pf6N2E2_1073 0.57 -0.9 1,253,115 - Pf6N2E2_1073 0.57 +0.6 1,253,184 - Pf6N2E2_1073 0.61 -1.5 1,253,184 - Pf6N2E2_1073 0.61 -1.0 1,253,184 - Pf6N2E2_1073 0.61 -0.3 1,253,184 - Pf6N2E2_1073 0.61 -2.5 1,253,270 + Pf6N2E2_1073 0.64 -0.0 1,253,271 - Pf6N2E2_1073 0.65 +0.8 1,253,306 + Pf6N2E2_1073 0.66 +0.1 1,253,306 + Pf6N2E2_1073 0.66 -0.9 1,253,307 - Pf6N2E2_1073 0.66 +0.5 1,253,315 + Pf6N2E2_1073 0.67 -1.5 1,253,315 + Pf6N2E2_1073 0.67 -1.3 1,253,316 - Pf6N2E2_1073 0.67 -0.7 1,253,316 - Pf6N2E2_1073 0.67 -0.5 1,253,316 - Pf6N2E2_1073 0.67 -0.8 1,253,316 - Pf6N2E2_1073 0.67 -1.1 1,253,446 - +1.2 1,253,507 + Pf6N2E2_1073 0.75 -0.2 1,253,507 + Pf6N2E2_1073 0.75 +0.6 1,253,765 + Pf6N2E2_1073 0.87 -0.7 1,253,780 + Pf6N2E2_1073 0.88 -0.7 1,253,780 + Pf6N2E2_1073 0.88 +0.5 1,253,780 + Pf6N2E2_1073 0.88 -0.9 1,253,780 + Pf6N2E2_1073 0.88 -0.8 1,253,780 + Pf6N2E2_1073 0.88 -1.3 1,253,780 + Pf6N2E2_1073 0.88 -2.2 1,253,780 + Pf6N2E2_1073 0.88 +0.8 1,253,780 + Pf6N2E2_1073 0.88 -0.8 1,253,780 + Pf6N2E2_1073 0.88 -1.7 1,253,781 - Pf6N2E2_1073 0.88 -0.8 1,253,781 - Pf6N2E2_1073 0.88 +1.8 1,253,781 - Pf6N2E2_1073 0.88 -0.3 1,253,819 + Pf6N2E2_1073 0.90 -1.3 1,253,834 + +0.8 1,253,834 + -1.8 1,254,036 + -0.8 1,254,036 + +0.6 1,254,036 + -0.5 1,254,036 + -2.1 1,254,036 + -1.3 1,254,037 - +0.0 1,254,037 - -1.1 1,254,132 + +0.1 1,254,133 - +0.9 1,254,133 - -0.8 1,254,560 - Pf6N2E2_1074 0.83 -0.1 1,254,583 - Pf6N2E2_1074 0.90 -1.6 1,254,850 + -1.9 1,254,850 + -0.3 1,254,851 - -2.8 1,254,851 - +1.8 1,254,851 - -0.8 1,254,955 + Pf6N2E2_1075 0.11 +0.2 1,254,955 + Pf6N2E2_1075 0.11 -0.0 1,254,956 - Pf6N2E2_1075 0.11 -0.2
Or see this region's nucleotide sequence