Experiment: m.b. Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides PGA1_c09100 and PGA1_c09110 overlap by 4 nucleotides
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
PGA1_c09110: PGA1_c09110 - thiamine biosynthesis protein ThiC, at 935,485 to 937,284
_c09110
Position (kb) 934
935 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100 at 935.351 kb on + strand, within PGA1_c09100 at 935.429 kb on - strand at 935.495 kb on + strand at 935.507 kb on + strand at 935.515 kb on - strand at 935.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.02 mM remove 933,484 + PGA1_c09050 0.88 -1.6 933,488 + PGA1_c09050 0.89 -0.7 933,518 + -2.9 933,575 + +1.0 933,580 + -0.7 933,581 - -2.6 933,583 - +0.4 933,583 - +0.5 933,583 - +0.5 933,583 - -1.1 933,603 - -2.0 933,689 + PGA1_c09070 0.15 -0.0 933,702 - PGA1_c09070 0.16 -1.3 933,731 + PGA1_c09070 0.20 -1.2 933,731 + PGA1_c09070 0.20 -2.2 933,739 - PGA1_c09070 0.21 -1.9 933,767 + PGA1_c09070 0.25 -2.1 933,862 + PGA1_c09070 0.37 -1.1 933,865 - PGA1_c09070 0.38 -0.7 933,878 + PGA1_c09070 0.39 -1.7 933,881 - PGA1_c09070 0.40 -0.3 933,901 - PGA1_c09070 0.42 -0.3 933,948 + PGA1_c09070 0.48 -0.9 933,972 + PGA1_c09070 0.51 -1.0 933,978 - PGA1_c09070 0.52 +0.2 933,980 - PGA1_c09070 0.52 -1.3 934,004 + PGA1_c09070 0.56 -1.4 934,004 + PGA1_c09070 0.56 -1.6 934,004 + PGA1_c09070 0.56 -3.0 934,004 + PGA1_c09070 0.56 -0.7 934,004 + PGA1_c09070 0.56 -1.2 934,004 + PGA1_c09070 0.56 -1.3 934,004 + PGA1_c09070 0.56 -2.4 934,004 + PGA1_c09070 0.56 -3.1 934,012 - PGA1_c09070 0.57 +0.9 934,012 - PGA1_c09070 0.57 -1.3 934,012 - PGA1_c09070 0.57 -3.4 934,012 - PGA1_c09070 0.57 -1.3 934,012 - PGA1_c09070 0.57 -0.9 934,012 - PGA1_c09070 0.57 +0.2 934,012 - PGA1_c09070 0.57 -1.0 934,012 - PGA1_c09070 0.57 +0.7 934,012 - PGA1_c09070 0.57 -1.3 934,076 + PGA1_c09070 0.65 -1.3 934,084 - PGA1_c09070 0.66 +0.0 934,103 - PGA1_c09070 0.68 -2.1 934,103 - PGA1_c09070 0.68 -1.9 934,108 + PGA1_c09070 0.69 -3.4 934,142 + PGA1_c09070 0.74 -2.4 934,142 + PGA1_c09070 0.74 -2.9 934,150 - PGA1_c09070 0.75 -1.7 934,152 + PGA1_c09070 0.75 -0.9 934,152 + PGA1_c09070 0.75 +0.2 934,162 + PGA1_c09070 0.76 -1.9 934,275 + -0.4 934,275 - -1.9 934,306 - -2.4 934,311 + -1.6 934,311 + -1.5 934,311 + -1.5 934,319 - +0.5 934,339 - -2.2 934,354 - +0.7 934,364 + -1.9 934,472 - PGA1_c09090 0.63 -0.6 934,513 - PGA1_c09090 0.83 -2.0 934,529 - -1.1 934,539 + -0.3 934,568 - -0.3 934,624 - PGA1_c09100 0.11 -2.1 934,636 - PGA1_c09100 0.13 -1.2 934,659 - PGA1_c09100 0.15 -1.5 934,659 - PGA1_c09100 0.15 -1.4 934,685 + PGA1_c09100 0.18 -1.0 934,685 + PGA1_c09100 0.18 +0.0 934,693 - PGA1_c09100 0.18 -1.3 934,693 - PGA1_c09100 0.18 -1.4 934,693 - PGA1_c09100 0.18 -1.4 934,710 + PGA1_c09100 0.20 -0.5 934,719 - PGA1_c09100 0.21 -2.3 934,846 + PGA1_c09100 0.34 -0.9 934,854 - PGA1_c09100 0.35 -0.3 934,854 - PGA1_c09100 0.35 -1.7 934,903 + PGA1_c09100 0.40 -0.9 934,943 + PGA1_c09100 0.44 -2.4 935,068 - PGA1_c09100 0.57 +1.5 935,081 + PGA1_c09100 0.58 -1.7 935,089 - PGA1_c09100 0.59 -2.1 935,139 + PGA1_c09100 0.64 -1.4 935,147 - PGA1_c09100 0.65 -2.4 935,147 - PGA1_c09100 0.65 -2.4 935,172 + PGA1_c09100 0.67 -0.9 935,177 + PGA1_c09100 0.68 -1.0 935,217 - PGA1_c09100 0.72 -0.7 935,228 - PGA1_c09100 0.73 -1.2 935,276 - PGA1_c09100 0.78 -1.9 935,351 + PGA1_c09100 0.86 -0.7 935,429 - +0.5 935,495 + -1.4 935,507 + -2.1 935,515 - -0.5 935,515 - -1.4
Or see this region's nucleotide sequence