Experiment: m.b. Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04930 and PGA1_c04940 are separated by 112 nucleotides PGA1_c04940 and PGA1_c04950 are separated by 296 nucleotides PGA1_c04950 and PGA1_c04960 are separated by 154 nucleotides
PGA1_c04930: PGA1_c04930 - tryptophan synthase alpha chain, at 484,569 to 485,360
_c04930
PGA1_c04940: PGA1_c04940 - Lipid A 3-O-deacylase (PagL)., at 485,473 to 485,985
_c04940
PGA1_c04950: PGA1_c04950 - GTP-dependent nucleic acid-binding protein EngD, at 486,282 to 487,379
_c04950
PGA1_c04960: PGA1_c04960 - putative non-specific ribonucleoside hydrolase, at 487,534 to 488,454
_c04960
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 485.363 kb on - strand at 485.392 kb on - strand at 485.412 kb on + strand at 485.420 kb on - strand at 485.420 kb on - strand at 485.426 kb on + strand at 485.453 kb on + strand at 485.517 kb on + strand at 485.522 kb on + strand at 485.530 kb on - strand, within PGA1_c04940 at 485.548 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.593 kb on + strand, within PGA1_c04940 at 485.604 kb on + strand, within PGA1_c04940 at 485.649 kb on + strand, within PGA1_c04940 at 485.676 kb on + strand, within PGA1_c04940 at 485.684 kb on - strand, within PGA1_c04940 at 485.740 kb on + strand, within PGA1_c04940 at 485.746 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.904 kb on - strand, within PGA1_c04940 at 485.943 kb on - strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.964 kb on + strand at 485.992 kb on + strand at 485.992 kb on + strand at 485.999 kb on - strand at 486.000 kb on - strand at 486.073 kb on - strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.140 kb on - strand at 486.173 kb on + strand at 486.190 kb on + strand at 486.202 kb on + strand at 486.212 kb on - strand at 486.216 kb on - strand at 486.234 kb on + strand at 486.244 kb on + strand at 486.255 kb on + strand at 486.273 kb on - strand at 486.520 kb on - strand, within PGA1_c04950 at 486.720 kb on + strand, within PGA1_c04950 at 486.969 kb on + strand, within PGA1_c04950 at 487.070 kb on + strand, within PGA1_c04950 at 487.381 kb on - strand at 487.383 kb on + strand at 487.454 kb on - strand at 487.472 kb on + strand at 487.480 kb on - strand at 487.509 kb on + strand at 487.509 kb on + strand at 487.517 kb on - strand at 487.566 kb on - strand at 487.573 kb on - strand at 487.610 kb on + strand at 487.653 kb on + strand, within PGA1_c04960 at 487.699 kb on + strand, within PGA1_c04960 at 487.700 kb on + strand, within PGA1_c04960 at 487.707 kb on - strand, within PGA1_c04960 at 487.708 kb on - strand, within PGA1_c04960 at 487.735 kb on + strand, within PGA1_c04960 at 487.759 kb on + strand, within PGA1_c04960 at 487.778 kb on + strand, within PGA1_c04960 at 487.820 kb on - strand, within PGA1_c04960 at 487.828 kb on - strand, within PGA1_c04960 at 487.832 kb on - strand, within PGA1_c04960 at 487.923 kb on - strand, within PGA1_c04960 at 487.935 kb on + strand, within PGA1_c04960 at 487.945 kb on + strand, within PGA1_c04960 at 487.959 kb on + strand, within PGA1_c04960 at 487.980 kb on - strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 488.083 kb on - strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.115 kb on - strand, within PGA1_c04960 at 488.126 kb on - strand, within PGA1_c04960 at 488.146 kb on + strand, within PGA1_c04960 at 488.159 kb on + strand, within PGA1_c04960 at 488.164 kb on + strand, within PGA1_c04960 at 488.167 kb on - strand, within PGA1_c04960 at 488.177 kb on - strand, within PGA1_c04960 at 488.284 kb on + strand, within PGA1_c04960 at 488.286 kb on + strand, within PGA1_c04960 at 488.329 kb on + strand, within PGA1_c04960 at 488.332 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.02 mM remove 485,363 - -1.8 485,392 - -1.3 485,412 + -0.7 485,420 - +0.2 485,420 - +0.0 485,426 + +0.3 485,453 + -2.3 485,517 + -0.4 485,522 + -1.0 485,530 - PGA1_c04940 0.11 +0.2 485,548 + PGA1_c04940 0.15 -0.0 485,557 + PGA1_c04940 0.16 -0.2 485,557 + PGA1_c04940 0.16 +0.5 485,557 + PGA1_c04940 0.16 -0.1 485,557 + PGA1_c04940 0.16 +0.2 485,557 + PGA1_c04940 0.16 -0.1 485,562 + PGA1_c04940 0.17 -1.8 485,562 + PGA1_c04940 0.17 +1.7 485,565 - PGA1_c04940 0.18 -1.2 485,565 - PGA1_c04940 0.18 +0.6 485,593 + PGA1_c04940 0.23 +0.5 485,604 + PGA1_c04940 0.26 +0.5 485,649 + PGA1_c04940 0.34 +0.1 485,676 + PGA1_c04940 0.40 -0.0 485,684 - PGA1_c04940 0.41 -2.6 485,740 + PGA1_c04940 0.52 +0.8 485,746 - PGA1_c04940 0.53 +0.4 485,766 - PGA1_c04940 0.57 -1.0 485,766 - PGA1_c04940 0.57 +3.1 485,853 + PGA1_c04940 0.74 -1.6 485,853 + PGA1_c04940 0.74 -1.1 485,904 - PGA1_c04940 0.84 +0.0 485,943 - -1.0 485,947 + -0.8 485,947 + +0.3 485,947 + +0.9 485,947 + -0.5 485,947 + -0.5 485,955 - -0.0 485,955 - +0.8 485,955 - +0.2 485,964 + -0.4 485,992 + -0.5 485,992 + +1.8 485,999 - +0.6 486,000 - -0.2 486,073 - -0.8 486,076 + -0.4 486,076 + +0.6 486,076 + -0.2 486,076 + -0.2 486,076 + +0.8 486,076 + -0.3 486,076 + -2.2 486,076 + +0.7 486,076 + +0.9 486,076 + -0.2 486,076 + -0.3 486,076 + -1.2 486,076 + +0.2 486,076 + +0.8 486,076 + -2.2 486,076 + +0.3 486,076 + -1.2 486,076 + +0.4 486,076 + -1.5 486,076 + -0.1 486,076 + +0.6 486,076 + -0.2 486,076 + -0.8 486,076 + +1.6 486,076 + -0.6 486,076 + -0.0 486,076 + -2.6 486,076 + -0.2 486,076 + -1.0 486,084 - +0.2 486,084 - +0.1 486,084 - +0.1 486,084 - -1.6 486,084 - -0.8 486,084 - +0.4 486,084 - -0.6 486,084 - -0.1 486,084 - -0.0 486,084 - -0.0 486,084 - -3.2 486,084 - -1.5 486,084 - -0.4 486,084 - -0.8 486,084 - -0.4 486,084 - -0.6 486,084 - -1.6 486,084 - +0.1 486,084 - -0.1 486,084 - -0.6 486,084 - -0.3 486,084 - +0.6 486,084 - -1.0 486,084 - +2.8 486,084 - -1.1 486,084 - +1.3 486,084 - +0.9 486,084 - +1.8 486,084 - +0.8 486,084 - -1.6 486,084 - -0.6 486,084 - -0.8 486,084 - -2.0 486,084 - -0.8 486,084 - +0.3 486,084 - -2.1 486,084 - -0.6 486,140 - -2.4 486,173 + -1.6 486,190 + +1.7 486,202 + -0.0 486,212 - -1.3 486,216 - +0.2 486,234 + +1.3 486,244 + -0.5 486,255 + -0.2 486,273 - +0.2 486,520 - PGA1_c04950 0.22 -2.1 486,720 + PGA1_c04950 0.40 -2.7 486,969 + PGA1_c04950 0.63 +0.8 487,070 + PGA1_c04950 0.72 -1.2 487,381 - +0.4 487,383 + -0.2 487,454 - +1.1 487,472 + +1.2 487,480 - -0.1 487,509 + -1.0 487,509 + +0.1 487,517 - +0.2 487,566 - +1.3 487,573 - +1.3 487,610 + -0.8 487,653 + PGA1_c04960 0.13 -0.7 487,699 + PGA1_c04960 0.18 -0.6 487,700 + PGA1_c04960 0.18 -1.6 487,707 - PGA1_c04960 0.19 -1.8 487,708 - PGA1_c04960 0.19 -1.1 487,735 + PGA1_c04960 0.22 -1.2 487,759 + PGA1_c04960 0.24 -3.5 487,778 + PGA1_c04960 0.26 -1.2 487,820 - PGA1_c04960 0.31 -0.8 487,828 - PGA1_c04960 0.32 -0.2 487,832 - PGA1_c04960 0.32 -0.1 487,923 - PGA1_c04960 0.42 -0.6 487,935 + PGA1_c04960 0.44 +0.2 487,945 + PGA1_c04960 0.45 +0.0 487,959 + PGA1_c04960 0.46 -2.1 487,980 - PGA1_c04960 0.48 -0.7 487,988 + PGA1_c04960 0.49 +2.8 487,988 + PGA1_c04960 0.49 -1.7 487,988 + PGA1_c04960 0.49 -0.9 487,996 - PGA1_c04960 0.50 +0.8 487,996 - PGA1_c04960 0.50 -0.5 487,996 - PGA1_c04960 0.50 -1.1 487,996 - PGA1_c04960 0.50 -1.0 488,083 - PGA1_c04960 0.60 +0.8 488,096 + PGA1_c04960 0.61 -1.6 488,096 + PGA1_c04960 0.61 -1.6 488,096 + PGA1_c04960 0.61 -1.6 488,096 + PGA1_c04960 0.61 -0.8 488,096 + PGA1_c04960 0.61 -0.7 488,096 + PGA1_c04960 0.61 -2.1 488,104 - PGA1_c04960 0.62 +0.2 488,104 - PGA1_c04960 0.62 -0.8 488,115 - PGA1_c04960 0.63 -2.1 488,126 - PGA1_c04960 0.64 -1.2 488,146 + PGA1_c04960 0.66 -1.6 488,159 + PGA1_c04960 0.68 -2.1 488,164 + PGA1_c04960 0.68 -0.1 488,167 - PGA1_c04960 0.69 -0.0 488,177 - PGA1_c04960 0.70 -2.6 488,284 + PGA1_c04960 0.81 -1.2 488,286 + PGA1_c04960 0.82 -0.9 488,329 + PGA1_c04960 0.86 -2.1 488,332 - PGA1_c04960 0.87 -1.7 488,337 - PGA1_c04960 0.87 -2.2 488,337 - PGA1_c04960 0.87 -0.1 488,366 + -1.1
Or see this region's nucleotide sequence