Experiment: m.b. Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04830 and PGA1_c04840 are separated by 344 nucleotides PGA1_c04840 and PGA1_c04850 overlap by 1 nucleotides PGA1_c04850 and PGA1_c04860 are separated by 7 nucleotides PGA1_c04860 and PGA1_c04870 are separated by 127 nucleotides
PGA1_c04830: PGA1_c04830 - 30S ribosomal protein S1, at 475,145 to 476,824
_c04830
PGA1_c04840: PGA1_c04840 - integration host factor subunit beta, at 477,169 to 477,453
_c04840
PGA1_c04850: PGA1_c04850 - Protein of unknown function (DUF1049)., at 477,453 to 477,803
_c04850
PGA1_c04860: PGA1_c04860 - N-(5'-phosphoribosyl)anthranilate isomerase TrpF, at 477,811 to 478,464
_c04860
PGA1_c04870: PGA1_c04870 - tryptophan synthase beta chain, at 478,592 to 479,845
_c04870
Position (kb) 477
478 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 476.899 kb on + strand at 476.992 kb on + strand at 477.039 kb on - strand at 477.070 kb on - strand at 477.075 kb on + strand at 477.080 kb on + strand at 477.083 kb on - strand at 477.083 kb on - strand at 477.145 kb on + strand at 477.475 kb on + strand at 477.545 kb on + strand, within PGA1_c04850 at 477.749 kb on + strand, within PGA1_c04850 at 477.767 kb on + strand, within PGA1_c04850 at 477.783 kb on + strand at 477.808 kb on + strand at 478.460 kb on + strand at 478.460 kb on + strand at 478.501 kb on - strand at 478.559 kb on - strand at 478.579 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.02 mM remove 476,899 + +0.8 476,992 + +0.3 477,039 - -0.4 477,070 - -1.6 477,075 + -1.4 477,080 + +0.8 477,083 - -0.6 477,083 - -0.1 477,145 + +0.1 477,475 + -1.8 477,545 + PGA1_c04850 0.26 -2.0 477,749 + PGA1_c04850 0.84 -2.8 477,767 + PGA1_c04850 0.89 -1.2 477,783 + -2.1 477,808 + +0.1 478,460 + -1.2 478,460 + -1.5 478,501 - -1.2 478,559 - -2.0 478,579 + +0.4
Or see this region's nucleotide sequence