Experiment: m.b. Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01640 and PGA1_c01650 are separated by 122 nucleotides PGA1_c01650 and PGA1_c01660 are separated by 67 nucleotides PGA1_c01660 and PGA1_c01670 are separated by 73 nucleotides PGA1_c01670 and PGA1_c01680 are separated by 205 nucleotides PGA1_c01680 and PGA1_c01690 are separated by 12 nucleotides
PGA1_c01640: PGA1_c01640 - Electron transfer DM13., at 157,621 to 158,088
_c01640
PGA1_c01650: PGA1_c01650 - conserved hypothetical protein, at 158,211 to 158,831
_c01650
PGA1_c01660: PGA1_c01660 - hypothetical protein, at 158,899 to 159,243
_c01660
PGA1_c01670: PGA1_c01670 - hypothetical protein, at 159,317 to 159,691
_c01670
PGA1_c01680: PGA1_c01680 - 50S ribosomal protein L29, at 159,897 to 160,097
_c01680
PGA1_c01690: PGA1_c01690 - 30S ribosomal protein S17, at 160,110 to 160,340
_c01690
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 157.943 kb on + strand, within PGA1_c01640 at 157.969 kb on + strand, within PGA1_c01640 at 157.993 kb on + strand, within PGA1_c01640 at 157.993 kb on + strand, within PGA1_c01640 at 157.993 kb on + strand, within PGA1_c01640 at 158.001 kb on - strand, within PGA1_c01640 at 158.016 kb on + strand, within PGA1_c01640 at 158.039 kb on - strand, within PGA1_c01640 at 158.049 kb on + strand at 158.057 kb on - strand at 158.070 kb on - strand at 158.070 kb on - strand at 158.106 kb on + strand at 158.114 kb on - strand at 158.114 kb on - strand at 158.115 kb on + strand at 158.119 kb on - strand at 158.123 kb on - strand at 158.140 kb on + strand at 158.190 kb on + strand at 158.198 kb on - strand at 158.198 kb on - strand at 158.203 kb on + strand at 158.203 kb on - strand at 158.279 kb on + strand, within PGA1_c01650 at 158.280 kb on - strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.337 kb on + strand, within PGA1_c01650 at 158.342 kb on + strand, within PGA1_c01650 at 158.342 kb on + strand, within PGA1_c01650 at 158.345 kb on - strand, within PGA1_c01650 at 158.345 kb on - strand, within PGA1_c01650 at 158.345 kb on - strand, within PGA1_c01650 at 158.345 kb on - strand, within PGA1_c01650 at 158.347 kb on + strand, within PGA1_c01650 at 158.347 kb on + strand, within PGA1_c01650 at 158.350 kb on - strand, within PGA1_c01650 at 158.350 kb on - strand, within PGA1_c01650 at 158.397 kb on + strand, within PGA1_c01650 at 158.397 kb on + strand, within PGA1_c01650 at 158.410 kb on - strand, within PGA1_c01650 at 158.502 kb on - strand, within PGA1_c01650 at 158.561 kb on + strand, within PGA1_c01650 at 158.561 kb on + strand, within PGA1_c01650 at 158.676 kb on + strand, within PGA1_c01650 at 158.686 kb on + strand, within PGA1_c01650 at 158.744 kb on - strand, within PGA1_c01650 at 158.769 kb on - strand, within PGA1_c01650 at 158.809 kb on + strand at 158.809 kb on + strand at 158.809 kb on + strand at 158.817 kb on - strand at 158.817 kb on - strand at 158.860 kb on + strand at 158.860 kb on + strand at 158.863 kb on + strand at 158.863 kb on + strand at 158.875 kb on + strand at 158.875 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.877 kb on + strand at 158.878 kb on + strand at 158.878 kb on + strand at 158.878 kb on + strand at 158.883 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.885 kb on - strand at 158.943 kb on + strand, within PGA1_c01660 at 159.047 kb on - strand, within PGA1_c01660 at 159.056 kb on + strand, within PGA1_c01660 at 159.081 kb on + strand, within PGA1_c01660 at 159.133 kb on + strand, within PGA1_c01660 at 159.133 kb on - strand, within PGA1_c01660 at 159.146 kb on - strand, within PGA1_c01660 at 159.146 kb on - strand, within PGA1_c01660 at 159.157 kb on + strand, within PGA1_c01660 at 159.159 kb on + strand, within PGA1_c01660 at 159.164 kb on - strand, within PGA1_c01660 at 159.167 kb on - strand, within PGA1_c01660 at 159.222 kb on + strand at 159.222 kb on + strand at 159.225 kb on - strand at 159.395 kb on - strand, within PGA1_c01670 at 159.395 kb on - strand, within PGA1_c01670 at 159.436 kb on - strand, within PGA1_c01670 at 159.538 kb on + strand, within PGA1_c01670 at 159.571 kb on - strand, within PGA1_c01670 at 159.576 kb on - strand, within PGA1_c01670 at 159.616 kb on - strand, within PGA1_c01670 at 159.661 kb on - strand at 159.674 kb on - strand at 159.674 kb on - strand at 159.676 kb on + strand at 159.676 kb on + strand at 159.683 kb on + strand at 159.696 kb on + strand at 159.714 kb on + strand at 159.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.02 mM remove 157,943 + PGA1_c01640 0.69 -0.3 157,969 + PGA1_c01640 0.74 -1.2 157,993 + PGA1_c01640 0.79 +0.6 157,993 + PGA1_c01640 0.79 -0.1 157,993 + PGA1_c01640 0.79 +0.1 158,001 - PGA1_c01640 0.81 +0.6 158,016 + PGA1_c01640 0.84 +0.1 158,039 - PGA1_c01640 0.89 -0.2 158,049 + +1.0 158,057 - +0.3 158,070 - +0.7 158,070 - -1.6 158,106 + -1.3 158,114 - +1.2 158,114 - +1.0 158,115 + -0.5 158,119 - +0.8 158,123 - +0.5 158,140 + -0.6 158,190 + -1.0 158,198 - +0.3 158,198 - -1.8 158,203 + +1.2 158,203 - -2.0 158,279 + PGA1_c01650 0.11 +1.0 158,280 - PGA1_c01650 0.11 -1.5 158,337 + PGA1_c01650 0.20 +0.3 158,337 + PGA1_c01650 0.20 -2.3 158,337 + PGA1_c01650 0.20 +3.4 158,337 + PGA1_c01650 0.20 +0.0 158,337 + PGA1_c01650 0.20 +0.6 158,337 + PGA1_c01650 0.20 -0.7 158,337 + PGA1_c01650 0.20 +1.4 158,342 + PGA1_c01650 0.21 +1.1 158,342 + PGA1_c01650 0.21 -1.2 158,345 - PGA1_c01650 0.22 +1.1 158,345 - PGA1_c01650 0.22 +0.1 158,345 - PGA1_c01650 0.22 +1.3 158,345 - PGA1_c01650 0.22 +0.1 158,347 + PGA1_c01650 0.22 +0.6 158,347 + PGA1_c01650 0.22 +0.8 158,350 - PGA1_c01650 0.22 -0.6 158,350 - PGA1_c01650 0.22 +0.8 158,397 + PGA1_c01650 0.30 -1.1 158,397 + PGA1_c01650 0.30 -0.2 158,410 - PGA1_c01650 0.32 -1.2 158,502 - PGA1_c01650 0.47 -2.8 158,561 + PGA1_c01650 0.56 -0.5 158,561 + PGA1_c01650 0.56 -0.6 158,676 + PGA1_c01650 0.75 -1.5 158,686 + PGA1_c01650 0.76 +0.6 158,744 - PGA1_c01650 0.86 -0.1 158,769 - PGA1_c01650 0.90 +0.3 158,809 + +0.1 158,809 + -1.7 158,809 + -0.2 158,817 - -0.5 158,817 - +2.3 158,860 + +0.3 158,860 + +1.2 158,863 + -1.2 158,863 + -0.4 158,875 + -0.1 158,875 + -0.9 158,877 + +0.4 158,877 + -1.8 158,877 + -0.3 158,877 + -1.8 158,877 + -0.5 158,877 + -3.2 158,877 + +1.9 158,877 + +0.3 158,877 + +0.2 158,877 + +0.8 158,877 + +0.7 158,877 + -0.5 158,878 + -0.5 158,878 + +0.8 158,878 + -0.9 158,883 - +1.5 158,885 - -0.1 158,885 - +0.4 158,885 - +1.1 158,885 - -2.4 158,885 - -0.3 158,885 - +1.2 158,885 - -0.2 158,943 + PGA1_c01660 0.13 -1.0 159,047 - PGA1_c01660 0.43 -0.8 159,056 + PGA1_c01660 0.46 -0.9 159,081 + PGA1_c01660 0.53 -3.0 159,133 + PGA1_c01660 0.68 +1.1 159,133 - PGA1_c01660 0.68 -1.0 159,146 - PGA1_c01660 0.72 -1.7 159,146 - PGA1_c01660 0.72 -0.3 159,157 + PGA1_c01660 0.75 -0.2 159,159 + PGA1_c01660 0.75 -2.4 159,164 - PGA1_c01660 0.77 +0.8 159,167 - PGA1_c01660 0.78 -0.8 159,222 + +0.6 159,222 + -1.1 159,225 - -1.7 159,395 - PGA1_c01670 0.21 -0.4 159,395 - PGA1_c01670 0.21 +0.4 159,436 - PGA1_c01670 0.32 -1.8 159,538 + PGA1_c01670 0.59 +0.6 159,571 - PGA1_c01670 0.68 +1.1 159,576 - PGA1_c01670 0.69 +0.9 159,616 - PGA1_c01670 0.80 +0.6 159,661 - +0.2 159,674 - +0.8 159,674 - -1.4 159,676 + -0.3 159,676 + +0.6 159,683 + +1.0 159,696 + -1.2 159,714 + +1.8 159,721 + +2.8
Or see this region's nucleotide sequence