Experiment: m.b. Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10790 and PGA1_c10800 are separated by 232 nucleotides PGA1_c10800 and PGA1_c10810 are separated by 124 nucleotides PGA1_c10810 and PGA1_c10820 are separated by 240 nucleotides
PGA1_c10790: PGA1_c10790 - Type IV pilus biogenesis., at 1,114,771 to 1,115,040
_c10790
PGA1_c10800: PGA1_c10800 - Uncharacterized protein conserved in bacteria (DUF2199)., at 1,115,273 to 1,115,815
_c10800
PGA1_c10810: PGA1_c10810 - amidophosphoribosyltransferase PurF, at 1,115,940 to 1,117,406
_c10810
PGA1_c10820: PGA1_c10820 - hypothetical protein, at 1,117,647 to 1,118,351
_c10820
Position (kb)
1115
1116
1117
1118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1114.990 kb on + strand, within PGA1_c10790 at 1114.998 kb on - strand, within PGA1_c10790 at 1115.120 kb on - strand at 1115.181 kb on + strand at 1115.181 kb on + strand at 1115.181 kb on + strand at 1115.201 kb on + strand at 1115.219 kb on - strand at 1115.267 kb on - strand at 1115.279 kb on - strand at 1115.321 kb on - strand at 1115.349 kb on + strand, within PGA1_c10800 at 1115.391 kb on - strand, within PGA1_c10800 at 1115.444 kb on - strand, within PGA1_c10800 at 1115.503 kb on - strand, within PGA1_c10800 at 1115.587 kb on + strand, within PGA1_c10800 at 1115.595 kb on - strand, within PGA1_c10800 at 1115.708 kb on - strand, within PGA1_c10800 at 1115.734 kb on + strand, within PGA1_c10800 at 1115.787 kb on - strand at 1115.845 kb on - strand at 1115.862 kb on - strand at 1115.900 kb on + strand at 1115.932 kb on - strand at 1115.932 kb on - strand at 1115.937 kb on + strand at 1115.961 kb on - strand at 1116.051 kb on + strand at 1116.098 kb on + strand, within PGA1_c10810 at 1116.106 kb on - strand, within PGA1_c10810 at 1116.108 kb on + strand, within PGA1_c10810 at 1116.116 kb on - strand, within PGA1_c10810 at 1116.170 kb on + strand, within PGA1_c10810 at 1116.206 kb on - strand, within PGA1_c10810 at 1116.283 kb on + strand, within PGA1_c10810 at 1116.336 kb on - strand, within PGA1_c10810 at 1116.343 kb on + strand, within PGA1_c10810 at 1116.478 kb on - strand, within PGA1_c10810 at 1116.495 kb on + strand, within PGA1_c10810 at 1116.513 kb on - strand, within PGA1_c10810 at 1116.695 kb on + strand, within PGA1_c10810 at 1116.743 kb on + strand, within PGA1_c10810 at 1116.770 kb on + strand, within PGA1_c10810 at 1116.770 kb on + strand, within PGA1_c10810 at 1116.832 kb on + strand, within PGA1_c10810 at 1116.840 kb on - strand, within PGA1_c10810 at 1116.903 kb on - strand, within PGA1_c10810 at 1116.918 kb on - strand, within PGA1_c10810 at 1116.962 kb on + strand, within PGA1_c10810 at 1117.018 kb on - strand, within PGA1_c10810 at 1117.020 kb on - strand, within PGA1_c10810 at 1117.075 kb on + strand, within PGA1_c10810 at 1117.153 kb on + strand, within PGA1_c10810 at 1117.173 kb on - strand, within PGA1_c10810 at 1117.178 kb on - strand, within PGA1_c10810 at 1117.222 kb on + strand, within PGA1_c10810 at 1117.230 kb on - strand, within PGA1_c10810 at 1117.230 kb on - strand, within PGA1_c10810 at 1117.394 kb on - strand at 1117.509 kb on - strand at 1117.537 kb on - strand at 1117.548 kb on + strand at 1117.565 kb on + strand at 1117.571 kb on + strand at 1117.583 kb on + strand at 1117.601 kb on + strand at 1117.611 kb on + strand at 1117.644 kb on + strand at 1117.678 kb on - strand at 1117.699 kb on - strand at 1117.780 kb on + strand, within PGA1_c10820 at 1117.798 kb on - strand, within PGA1_c10820 at 1117.798 kb on - strand, within PGA1_c10820 at 1117.835 kb on - strand, within PGA1_c10820 at 1117.867 kb on - strand, within PGA1_c10820 at 1118.099 kb on - strand, within PGA1_c10820 at 1118.107 kb on + strand, within PGA1_c10820 at 1118.121 kb on + strand, within PGA1_c10820 at 1118.139 kb on + strand, within PGA1_c10820 at 1118.236 kb on - strand, within PGA1_c10820 at 1118.259 kb on + strand, within PGA1_c10820 at 1118.259 kb on + strand, within PGA1_c10820 at 1118.275 kb on - strand, within PGA1_c10820 at 1118.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzethonium chloride 0.02 mM remove 1,114,990 + PGA1_c10790 0.81 +0.6 1,114,998 - PGA1_c10790 0.84 +0.6 1,115,120 - +0.1 1,115,181 + +0.9 1,115,181 + +0.4 1,115,181 + +0.9 1,115,201 + -0.9 1,115,219 - -0.1 1,115,267 - +0.9 1,115,279 - +1.5 1,115,321 - -0.9 1,115,349 + PGA1_c10800 0.14 -0.8 1,115,391 - PGA1_c10800 0.22 -1.4 1,115,444 - PGA1_c10800 0.31 +0.2 1,115,503 - PGA1_c10800 0.42 +0.3 1,115,587 + PGA1_c10800 0.58 -0.4 1,115,595 - PGA1_c10800 0.59 +0.1 1,115,708 - PGA1_c10800 0.80 -0.5 1,115,734 + PGA1_c10800 0.85 -0.8 1,115,787 - +0.0 1,115,845 - +0.5 1,115,862 - +0.9 1,115,900 + -0.8 1,115,932 - -0.0 1,115,932 - -0.4 1,115,937 + -0.7 1,115,961 - -1.6 1,116,051 + -0.3 1,116,098 + PGA1_c10810 0.11 -1.1 1,116,106 - PGA1_c10810 0.11 -1.0 1,116,108 + PGA1_c10810 0.11 -0.8 1,116,116 - PGA1_c10810 0.12 -2.8 1,116,170 + PGA1_c10810 0.16 -0.8 1,116,206 - PGA1_c10810 0.18 -2.4 1,116,283 + PGA1_c10810 0.23 -2.2 1,116,336 - PGA1_c10810 0.27 -3.0 1,116,343 + PGA1_c10810 0.27 -2.4 1,116,478 - PGA1_c10810 0.37 -1.8 1,116,495 + PGA1_c10810 0.38 -1.8 1,116,513 - PGA1_c10810 0.39 -1.6 1,116,695 + PGA1_c10810 0.51 -3.8 1,116,743 + PGA1_c10810 0.55 -1.8 1,116,770 + PGA1_c10810 0.57 +1.2 1,116,770 + PGA1_c10810 0.57 -2.3 1,116,832 + PGA1_c10810 0.61 -2.8 1,116,840 - PGA1_c10810 0.61 -1.3 1,116,903 - PGA1_c10810 0.66 -3.4 1,116,918 - PGA1_c10810 0.67 -0.1 1,116,962 + PGA1_c10810 0.70 -2.4 1,117,018 - PGA1_c10810 0.73 -2.7 1,117,020 - PGA1_c10810 0.74 +0.7 1,117,075 + PGA1_c10810 0.77 -1.4 1,117,153 + PGA1_c10810 0.83 -1.0 1,117,173 - PGA1_c10810 0.84 -2.3 1,117,178 - PGA1_c10810 0.84 -3.3 1,117,222 + PGA1_c10810 0.87 -1.3 1,117,230 - PGA1_c10810 0.88 -2.5 1,117,230 - PGA1_c10810 0.88 -2.5 1,117,394 - -1.1 1,117,509 - +1.3 1,117,537 - -0.1 1,117,548 + +0.7 1,117,565 + -1.8 1,117,571 + -0.1 1,117,583 + +0.7 1,117,601 + +0.1 1,117,611 + +0.7 1,117,644 + +1.5 1,117,678 - +0.4 1,117,699 - +1.3 1,117,780 + PGA1_c10820 0.19 +0.2 1,117,798 - PGA1_c10820 0.21 -1.7 1,117,798 - PGA1_c10820 0.21 -0.2 1,117,835 - PGA1_c10820 0.27 -2.6 1,117,867 - PGA1_c10820 0.31 -0.9 1,118,099 - PGA1_c10820 0.64 -0.8 1,118,107 + PGA1_c10820 0.65 +0.4 1,118,121 + PGA1_c10820 0.67 -1.3 1,118,139 + PGA1_c10820 0.70 -1.6 1,118,236 - PGA1_c10820 0.84 +0.6 1,118,259 + PGA1_c10820 0.87 -0.2 1,118,259 + PGA1_c10820 0.87 +0.9 1,118,275 - PGA1_c10820 0.89 +1.7 1,118,361 - -0.8
Or see this region's nucleotide sequence