Experiment: L-Arginine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15205 and BWI76_RS15210 are separated by 105 nucleotides BWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotides BWI76_RS15215 and BWI76_RS15220 are separated by 145 nucleotides
BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528
_RS15205
BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512
_RS15210
BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948
_RS15215
BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038
_RS15220
Position (kb) 3188
3189
3190 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3188.031 kb on + strand, within BWI76_RS15205 at 3188.691 kb on - strand at 3188.970 kb on - strand, within BWI76_RS15210 at 3189.178 kb on + strand, within BWI76_RS15210 at 3189.322 kb on + strand, within BWI76_RS15210 at 3189.351 kb on - strand, within BWI76_RS15210 at 3189.595 kb on + strand, within BWI76_RS15215 at 3190.233 kb on + strand, within BWI76_RS15220
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arginine (N) remove 3,188,031 + BWI76_RS15205 0.64 -0.1 3,188,691 - -0.3 3,188,970 - BWI76_RS15210 0.38 +0.1 3,189,178 + BWI76_RS15210 0.62 -0.1 3,189,322 + BWI76_RS15210 0.78 -0.5 3,189,351 - BWI76_RS15210 0.82 -0.7 3,189,595 + BWI76_RS15215 0.27 -4.0 3,190,233 + BWI76_RS15220 0.15 -0.1
Or see this region's nucleotide sequence