Experiment: PYE_noCalcium 25C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01503 and CCNA_01502 overlap by 2 nucleotides CCNA_01502 and CCNA_01504 are separated by 8 nucleotides CCNA_01504 and CCNA_01505 are separated by 42 nucleotides CCNA_01505 and CCNA_01506 are separated by 65 nucleotides
CCNA_01503: CCNA_01503 - phosphotransferase family protein, at 1,612,264 to 1,613,346
_01503
CCNA_01502: CCNA_01502 - conserved membrane spanning protein, at 1,613,345 to 1,614,289
_01502
CCNA_01504: CCNA_01504 - ATP-dependent DNA helicase recG, at 1,614,298 to 1,616,394
_01504
CCNA_01505: CCNA_01505 - hypothetical protein, at 1,616,437 to 1,616,739
_01505
CCNA_01506: CCNA_01506 - cystine transport ATP-binding protein, at 1,616,805 to 1,617,545
_01506
Position (kb)
1614
1615
1616
1617 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1613.307 kb on + strand at 1613.310 kb on - strand at 1613.326 kb on + strand at 1613.326 kb on + strand at 1613.326 kb on + strand at 1613.334 kb on - strand at 1613.357 kb on - strand at 1613.408 kb on + strand at 1613.408 kb on + strand at 1613.448 kb on - strand, within CCNA_01502 at 1613.543 kb on + strand, within CCNA_01502 at 1613.592 kb on + strand, within CCNA_01502 at 1613.669 kb on - strand, within CCNA_01502 at 1613.689 kb on - strand, within CCNA_01502 at 1613.691 kb on + strand, within CCNA_01502 at 1613.781 kb on + strand, within CCNA_01502 at 1613.781 kb on + strand, within CCNA_01502 at 1613.786 kb on + strand, within CCNA_01502 at 1613.787 kb on + strand, within CCNA_01502 at 1613.792 kb on + strand, within CCNA_01502 at 1613.799 kb on - strand, within CCNA_01502 at 1613.801 kb on + strand, within CCNA_01502 at 1613.801 kb on + strand, within CCNA_01502 at 1613.837 kb on + strand, within CCNA_01502 at 1613.837 kb on + strand, within CCNA_01502 at 1613.845 kb on - strand, within CCNA_01502 at 1613.845 kb on - strand, within CCNA_01502 at 1613.845 kb on - strand, within CCNA_01502 at 1613.857 kb on + strand, within CCNA_01502 at 1613.864 kb on + strand, within CCNA_01502 at 1613.865 kb on + strand, within CCNA_01502 at 1613.870 kb on + strand, within CCNA_01502 at 1613.870 kb on + strand, within CCNA_01502 at 1613.873 kb on - strand, within CCNA_01502 at 1613.875 kb on + strand, within CCNA_01502 at 1613.888 kb on - strand, within CCNA_01502 at 1613.948 kb on + strand, within CCNA_01502 at 1613.951 kb on - strand, within CCNA_01502 at 1613.953 kb on + strand, within CCNA_01502 at 1613.988 kb on + strand, within CCNA_01502 at 1613.998 kb on + strand, within CCNA_01502 at 1613.998 kb on + strand, within CCNA_01502 at 1613.998 kb on + strand, within CCNA_01502 at 1613.998 kb on + strand, within CCNA_01502 at 1613.998 kb on + strand, within CCNA_01502 at 1614.006 kb on - strand, within CCNA_01502 at 1614.006 kb on - strand, within CCNA_01502 at 1614.006 kb on - strand, within CCNA_01502 at 1614.018 kb on + strand, within CCNA_01502 at 1614.055 kb on + strand, within CCNA_01502 at 1614.055 kb on + strand, within CCNA_01502 at 1614.063 kb on - strand, within CCNA_01502 at 1614.095 kb on - strand, within CCNA_01502 at 1614.095 kb on - strand, within CCNA_01502 at 1614.105 kb on - strand, within CCNA_01502 at 1614.118 kb on + strand, within CCNA_01502 at 1614.168 kb on - strand, within CCNA_01502 at 1614.172 kb on + strand, within CCNA_01502 at 1614.241 kb on - strand at 1614.242 kb on + strand at 1614.261 kb on - strand at 1614.288 kb on + strand at 1614.291 kb on - strand at 1614.296 kb on - strand at 1614.296 kb on - strand at 1614.305 kb on + strand at 1614.310 kb on + strand at 1614.351 kb on - strand at 1614.355 kb on + strand at 1614.379 kb on + strand at 1614.379 kb on + strand at 1614.403 kb on + strand at 1614.489 kb on + strand at 1614.530 kb on - strand, within CCNA_01504 at 1614.552 kb on - strand, within CCNA_01504 at 1614.583 kb on + strand, within CCNA_01504 at 1614.606 kb on - strand, within CCNA_01504 at 1614.651 kb on + strand, within CCNA_01504 at 1614.732 kb on + strand, within CCNA_01504 at 1614.732 kb on + strand, within CCNA_01504 at 1614.732 kb on + strand, within CCNA_01504 at 1614.733 kb on + strand, within CCNA_01504 at 1614.743 kb on + strand, within CCNA_01504 at 1614.765 kb on + strand, within CCNA_01504 at 1614.804 kb on - strand, within CCNA_01504 at 1614.819 kb on - strand, within CCNA_01504 at 1614.885 kb on + strand, within CCNA_01504 at 1614.893 kb on - strand, within CCNA_01504 at 1614.893 kb on - strand, within CCNA_01504 at 1614.906 kb on - strand, within CCNA_01504 at 1615.029 kb on - strand, within CCNA_01504 at 1615.083 kb on - strand, within CCNA_01504 at 1615.124 kb on + strand, within CCNA_01504 at 1615.137 kb on - strand, within CCNA_01504 at 1615.154 kb on + strand, within CCNA_01504 at 1615.173 kb on - strand, within CCNA_01504 at 1615.191 kb on + strand, within CCNA_01504 at 1615.219 kb on + strand, within CCNA_01504 at 1615.224 kb on + strand, within CCNA_01504 at 1615.224 kb on + strand, within CCNA_01504 at 1615.227 kb on - strand, within CCNA_01504 at 1615.227 kb on - strand, within CCNA_01504 at 1615.232 kb on - strand, within CCNA_01504 at 1615.232 kb on - strand, within CCNA_01504 at 1615.268 kb on - strand, within CCNA_01504 at 1615.292 kb on + strand, within CCNA_01504 at 1615.374 kb on - strand, within CCNA_01504 at 1615.374 kb on - strand, within CCNA_01504 at 1615.449 kb on + strand, within CCNA_01504 at 1615.508 kb on + strand, within CCNA_01504 at 1615.518 kb on + strand, within CCNA_01504 at 1615.574 kb on + strand, within CCNA_01504 at 1615.582 kb on - strand, within CCNA_01504 at 1615.630 kb on - strand, within CCNA_01504 at 1615.676 kb on + strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.684 kb on - strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.857 kb on + strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.865 kb on - strand, within CCNA_01504 at 1615.878 kb on - strand, within CCNA_01504 at 1615.914 kb on + strand, within CCNA_01504 at 1615.936 kb on - strand, within CCNA_01504 at 1615.945 kb on - strand, within CCNA_01504 at 1615.945 kb on - strand, within CCNA_01504 at 1615.961 kb on + strand, within CCNA_01504 at 1615.961 kb on + strand, within CCNA_01504 at 1615.961 kb on + strand, within CCNA_01504 at 1615.969 kb on - strand, within CCNA_01504 at 1615.969 kb on - strand, within CCNA_01504 at 1615.969 kb on - strand, within CCNA_01504 at 1616.046 kb on - strand, within CCNA_01504 at 1616.060 kb on + strand, within CCNA_01504 at 1616.060 kb on + strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.068 kb on - strand, within CCNA_01504 at 1616.070 kb on - strand, within CCNA_01504 at 1616.097 kb on + strand, within CCNA_01504 at 1616.105 kb on - strand, within CCNA_01504 at 1616.105 kb on - strand, within CCNA_01504 at 1616.110 kb on - strand, within CCNA_01504 at 1616.130 kb on + strand, within CCNA_01504 at 1616.157 kb on + strand, within CCNA_01504 at 1616.165 kb on - strand, within CCNA_01504 at 1616.165 kb on - strand, within CCNA_01504 at 1616.211 kb on - strand at 1616.212 kb on - strand at 1616.237 kb on - strand at 1616.254 kb on + strand at 1616.262 kb on - strand at 1616.262 kb on - strand at 1616.267 kb on - strand at 1616.300 kb on - strand at 1616.300 kb on - strand at 1616.317 kb on - strand at 1616.343 kb on - strand at 1616.368 kb on - strand at 1616.444 kb on + strand at 1616.483 kb on + strand, within CCNA_01505 at 1616.493 kb on - strand, within CCNA_01505 at 1616.559 kb on + strand, within CCNA_01505 at 1616.581 kb on - strand, within CCNA_01505 at 1616.596 kb on + strand, within CCNA_01505 at 1616.604 kb on - strand, within CCNA_01505 at 1616.673 kb on - strand, within CCNA_01505 at 1616.682 kb on - strand, within CCNA_01505 at 1616.741 kb on - strand at 1616.741 kb on - strand at 1616.741 kb on - strand at 1616.751 kb on - strand at 1616.780 kb on - strand at 1616.788 kb on - strand at 1616.827 kb on - strand at 1616.852 kb on - strand at 1616.869 kb on - strand at 1616.892 kb on + strand, within CCNA_01506 at 1616.894 kb on - strand, within CCNA_01506 at 1616.900 kb on - strand, within CCNA_01506 at 1616.965 kb on - strand, within CCNA_01506 at 1617.022 kb on - strand, within CCNA_01506 at 1617.022 kb on - strand, within CCNA_01506 at 1617.029 kb on + strand, within CCNA_01506 at 1617.037 kb on - strand, within CCNA_01506 at 1617.054 kb on - strand, within CCNA_01506 at 1617.092 kb on - strand, within CCNA_01506 at 1617.119 kb on - strand, within CCNA_01506 at 1617.139 kb on + strand, within CCNA_01506 at 1617.147 kb on + strand, within CCNA_01506 at 1617.153 kb on - strand, within CCNA_01506 at 1617.183 kb on + strand, within CCNA_01506 at 1617.191 kb on - strand, within CCNA_01506 at 1617.252 kb on + strand, within CCNA_01506 at 1617.252 kb on + strand, within CCNA_01506 at 1617.252 kb on + strand, within CCNA_01506 at 1617.252 kb on + strand, within CCNA_01506 at 1617.257 kb on + strand, within CCNA_01506 at 1617.259 kb on + strand, within CCNA_01506 at 1617.260 kb on - strand, within CCNA_01506 at 1617.260 kb on - strand, within CCNA_01506 at 1617.285 kb on + strand, within CCNA_01506 at 1617.286 kb on - strand, within CCNA_01506 at 1617.286 kb on - strand, within CCNA_01506 at 1617.298 kb on + strand at 1617.312 kb on + strand, within CCNA_01506 at 1617.319 kb on + strand, within CCNA_01506 at 1617.321 kb on - strand, within CCNA_01506 at 1617.327 kb on - strand, within CCNA_01506 at 1617.327 kb on - strand, within CCNA_01506 at 1617.327 kb on - strand, within CCNA_01506 at 1617.332 kb on - strand, within CCNA_01506 at 1617.332 kb on - strand, within CCNA_01506 at 1617.332 kb on - strand, within CCNA_01506
Per-strain Table
Position Strand Gene LocusTag Fraction PYE_noCalcium 25C survival rep2 12 days remove 1,613,307 + +0.4 1,613,310 - -0.2 1,613,326 + -1.2 1,613,326 + -0.5 1,613,326 + -1.0 1,613,334 - -1.2 1,613,357 - -1.7 1,613,408 + -1.5 1,613,408 + -2.0 1,613,448 - CCNA_01502 0.11 +0.2 1,613,543 + CCNA_01502 0.21 -1.1 1,613,592 + CCNA_01502 0.26 -0.3 1,613,669 - CCNA_01502 0.34 +0.7 1,613,689 - CCNA_01502 0.36 -0.3 1,613,691 + CCNA_01502 0.37 -0.9 1,613,781 + CCNA_01502 0.46 -1.5 1,613,781 + CCNA_01502 0.46 -1.0 1,613,786 + CCNA_01502 0.47 -1.4 1,613,787 + CCNA_01502 0.47 +0.4 1,613,792 + CCNA_01502 0.47 +0.6 1,613,799 - CCNA_01502 0.48 -1.4 1,613,801 + CCNA_01502 0.48 -0.1 1,613,801 + CCNA_01502 0.48 +0.9 1,613,837 + CCNA_01502 0.52 +0.2 1,613,837 + CCNA_01502 0.52 +0.3 1,613,845 - CCNA_01502 0.53 -0.8 1,613,845 - CCNA_01502 0.53 +0.5 1,613,845 - CCNA_01502 0.53 -1.0 1,613,857 + CCNA_01502 0.54 +1.0 1,613,864 + CCNA_01502 0.55 +0.3 1,613,865 + CCNA_01502 0.55 -1.4 1,613,870 + CCNA_01502 0.56 -1.3 1,613,870 + CCNA_01502 0.56 -0.2 1,613,873 - CCNA_01502 0.56 -1.5 1,613,875 + CCNA_01502 0.56 +0.9 1,613,888 - CCNA_01502 0.57 +1.0 1,613,948 + CCNA_01502 0.64 -0.6 1,613,951 - CCNA_01502 0.64 -1.8 1,613,953 + CCNA_01502 0.64 +0.1 1,613,988 + CCNA_01502 0.68 -1.6 1,613,998 + CCNA_01502 0.69 +0.2 1,613,998 + CCNA_01502 0.69 +0.3 1,613,998 + CCNA_01502 0.69 -0.5 1,613,998 + CCNA_01502 0.69 +0.8 1,613,998 + CCNA_01502 0.69 +0.2 1,614,006 - CCNA_01502 0.70 +1.0 1,614,006 - CCNA_01502 0.70 -2.3 1,614,006 - CCNA_01502 0.70 -0.0 1,614,018 + CCNA_01502 0.71 +0.4 1,614,055 + CCNA_01502 0.75 +0.6 1,614,055 + CCNA_01502 0.75 +0.0 1,614,063 - CCNA_01502 0.76 +0.6 1,614,095 - CCNA_01502 0.79 +0.6 1,614,095 - CCNA_01502 0.79 -1.1 1,614,105 - CCNA_01502 0.80 -1.1 1,614,118 + CCNA_01502 0.82 +0.5 1,614,168 - CCNA_01502 0.87 -1.5 1,614,172 + CCNA_01502 0.88 -0.9 1,614,241 - -1.5 1,614,242 + +0.8 1,614,261 - +0.6 1,614,288 + +0.8 1,614,291 - -2.0 1,614,296 - +0.7 1,614,296 - +0.8 1,614,305 + -1.6 1,614,310 + -1.5 1,614,351 - -3.0 1,614,355 + -1.3 1,614,379 + -0.0 1,614,379 + -0.3 1,614,403 + -0.3 1,614,489 + -0.0 1,614,530 - CCNA_01504 0.11 -3.3 1,614,552 - CCNA_01504 0.12 -3.1 1,614,583 + CCNA_01504 0.14 +0.2 1,614,606 - CCNA_01504 0.15 -2.3 1,614,651 + CCNA_01504 0.17 +0.2 1,614,732 + CCNA_01504 0.21 -1.6 1,614,732 + CCNA_01504 0.21 -1.3 1,614,732 + CCNA_01504 0.21 -2.4 1,614,733 + CCNA_01504 0.21 +2.5 1,614,743 + CCNA_01504 0.21 -1.0 1,614,765 + CCNA_01504 0.22 -3.3 1,614,804 - CCNA_01504 0.24 -3.9 1,614,819 - CCNA_01504 0.25 -3.4 1,614,885 + CCNA_01504 0.28 -1.6 1,614,893 - CCNA_01504 0.28 -1.2 1,614,893 - CCNA_01504 0.28 -2.3 1,614,906 - CCNA_01504 0.29 -0.6 1,615,029 - CCNA_01504 0.35 -2.8 1,615,083 - CCNA_01504 0.37 -3.5 1,615,124 + CCNA_01504 0.39 +1.0 1,615,137 - CCNA_01504 0.40 -2.1 1,615,154 + CCNA_01504 0.41 -0.9 1,615,173 - CCNA_01504 0.42 -2.1 1,615,191 + CCNA_01504 0.43 -3.5 1,615,219 + CCNA_01504 0.44 -1.7 1,615,224 + CCNA_01504 0.44 -2.4 1,615,224 + CCNA_01504 0.44 -1.2 1,615,227 - CCNA_01504 0.44 -2.5 1,615,227 - CCNA_01504 0.44 -3.4 1,615,232 - CCNA_01504 0.45 -2.9 1,615,232 - CCNA_01504 0.45 -2.8 1,615,268 - CCNA_01504 0.46 -0.8 1,615,292 + CCNA_01504 0.47 -0.0 1,615,374 - CCNA_01504 0.51 -1.8 1,615,374 - CCNA_01504 0.51 -3.5 1,615,449 + CCNA_01504 0.55 -2.6 1,615,508 + CCNA_01504 0.58 -1.0 1,615,518 + CCNA_01504 0.58 -0.0 1,615,574 + CCNA_01504 0.61 -2.0 1,615,582 - CCNA_01504 0.61 -1.5 1,615,630 - CCNA_01504 0.64 -2.0 1,615,676 + CCNA_01504 0.66 -2.2 1,615,684 - CCNA_01504 0.66 -1.9 1,615,684 - CCNA_01504 0.66 -1.5 1,615,684 - CCNA_01504 0.66 -1.4 1,615,684 - CCNA_01504 0.66 -2.0 1,615,684 - CCNA_01504 0.66 -1.7 1,615,684 - CCNA_01504 0.66 -3.8 1,615,684 - CCNA_01504 0.66 -2.8 1,615,857 + CCNA_01504 0.74 +0.5 1,615,857 + CCNA_01504 0.74 +1.0 1,615,857 + CCNA_01504 0.74 -0.8 1,615,865 - CCNA_01504 0.75 -1.5 1,615,865 - CCNA_01504 0.75 -2.5 1,615,865 - CCNA_01504 0.75 -2.5 1,615,865 - CCNA_01504 0.75 -1.4 1,615,865 - CCNA_01504 0.75 -0.0 1,615,878 - CCNA_01504 0.75 -2.2 1,615,914 + CCNA_01504 0.77 -0.5 1,615,936 - CCNA_01504 0.78 -1.0 1,615,945 - CCNA_01504 0.79 -1.5 1,615,945 - CCNA_01504 0.79 -1.5 1,615,961 + CCNA_01504 0.79 +0.2 1,615,961 + CCNA_01504 0.79 -0.0 1,615,961 + CCNA_01504 0.79 -1.7 1,615,969 - CCNA_01504 0.80 -3.5 1,615,969 - CCNA_01504 0.80 -0.6 1,615,969 - CCNA_01504 0.80 -1.7 1,616,046 - CCNA_01504 0.83 -1.8 1,616,060 + CCNA_01504 0.84 +1.0 1,616,060 + CCNA_01504 0.84 -1.0 1,616,068 - CCNA_01504 0.84 -3.9 1,616,068 - CCNA_01504 0.84 -3.5 1,616,068 - CCNA_01504 0.84 -1.5 1,616,068 - CCNA_01504 0.84 -3.6 1,616,068 - CCNA_01504 0.84 -2.1 1,616,068 - CCNA_01504 0.84 -2.8 1,616,070 - CCNA_01504 0.85 -2.7 1,616,097 + CCNA_01504 0.86 -1.5 1,616,105 - CCNA_01504 0.86 -0.3 1,616,105 - CCNA_01504 0.86 -0.0 1,616,110 - CCNA_01504 0.86 -2.8 1,616,130 + CCNA_01504 0.87 -2.2 1,616,157 + CCNA_01504 0.89 -0.6 1,616,165 - CCNA_01504 0.89 -2.0 1,616,165 - CCNA_01504 0.89 -0.6 1,616,211 - -3.4 1,616,212 - -2.0 1,616,237 - -1.5 1,616,254 + -0.5 1,616,262 - -0.8 1,616,262 - -3.0 1,616,267 - -2.5 1,616,300 - -1.8 1,616,300 - -3.4 1,616,317 - -3.5 1,616,343 - -1.0 1,616,368 - -4.7 1,616,444 + +1.0 1,616,483 + CCNA_01505 0.15 -0.0 1,616,493 - CCNA_01505 0.18 +1.0 1,616,559 + CCNA_01505 0.40 -1.5 1,616,581 - CCNA_01505 0.48 +0.9 1,616,596 + CCNA_01505 0.52 -1.2 1,616,604 - CCNA_01505 0.55 +2.2 1,616,673 - CCNA_01505 0.78 +0.3 1,616,682 - CCNA_01505 0.81 +1.2 1,616,741 - -0.5 1,616,741 - +0.4 1,616,741 - +0.2 1,616,751 - +0.4 1,616,780 - -1.1 1,616,788 - +0.9 1,616,827 - +1.1 1,616,852 - -1.1 1,616,869 - +0.9 1,616,892 + CCNA_01506 0.12 -1.2 1,616,894 - CCNA_01506 0.12 +0.7 1,616,900 - CCNA_01506 0.13 +1.0 1,616,965 - CCNA_01506 0.22 +0.9 1,617,022 - CCNA_01506 0.29 +1.3 1,617,022 - CCNA_01506 0.29 -0.4 1,617,029 + CCNA_01506 0.30 -0.6 1,617,037 - CCNA_01506 0.31 +0.8 1,617,054 - CCNA_01506 0.34 +0.6 1,617,092 - CCNA_01506 0.39 -0.1 1,617,119 - CCNA_01506 0.42 +1.5 1,617,139 + CCNA_01506 0.45 +0.6 1,617,147 + CCNA_01506 0.46 +1.8 1,617,153 - CCNA_01506 0.47 +3.1 1,617,183 + CCNA_01506 0.51 +2.0 1,617,191 - CCNA_01506 0.52 +0.5 1,617,252 + CCNA_01506 0.60 -0.5 1,617,252 + CCNA_01506 0.60 +0.2 1,617,252 + CCNA_01506 0.60 -0.0 1,617,252 + CCNA_01506 0.60 +0.2 1,617,257 + CCNA_01506 0.61 -0.2 1,617,259 + CCNA_01506 0.61 +1.5 1,617,260 - CCNA_01506 0.61 -0.1 1,617,260 - CCNA_01506 0.61 +1.5 1,617,285 + CCNA_01506 0.65 -0.3 1,617,286 - CCNA_01506 0.65 -1.9 1,617,286 - CCNA_01506 0.65 -0.2 1,617,298 + +1.5 1,617,312 + CCNA_01506 0.68 +0.7 1,617,319 + CCNA_01506 0.69 -0.8 1,617,321 - CCNA_01506 0.70 +0.4 1,617,327 - CCNA_01506 0.70 +1.3 1,617,327 - CCNA_01506 0.70 +2.4 1,617,327 - CCNA_01506 0.70 +1.5 1,617,332 - CCNA_01506 0.71 +0.1 1,617,332 - CCNA_01506 0.71 +0.4 1,617,332 - CCNA_01506 0.71 -0.3
Or see this region's nucleotide sequence