Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18955

Experiment: Urea (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18945 and BWI76_RS18950 overlap by 19 nucleotidesBWI76_RS18950 and BWI76_RS18955 overlap by 7 nucleotidesBWI76_RS18955 and BWI76_RS18960 are separated by 83 nucleotides BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291 _RS18945 BWI76_RS18950: BWI76_RS18950 - imidazole glycerol phosphate synthase cyclase subunit, at 3,930,273 to 3,931,049 _RS18950 BWI76_RS18955: BWI76_RS18955 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 3,931,043 to 3,931,642 _RS18955 BWI76_RS18960: BWI76_RS18960 - glycosyl transferase, at 3,931,726 to 3,933,045 _RS18960 Position (kb) 3931 3932Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3930.057 kb on + strand, within BWI76_RS18945at 3930.223 kb on - strandat 3930.377 kb on + strand, within BWI76_RS18950at 3930.426 kb on + strand, within BWI76_RS18950at 3930.445 kb on + strand, within BWI76_RS18950at 3930.909 kb on + strand, within BWI76_RS18950at 3931.121 kb on + strand, within BWI76_RS18955at 3931.498 kb on - strand, within BWI76_RS18955at 3931.509 kb on + strand, within BWI76_RS18955at 3931.517 kb on + strand, within BWI76_RS18955at 3931.517 kb on + strand, within BWI76_RS18955at 3931.640 kb on - strandat 3931.653 kb on - strandat 3931.674 kb on + strandat 3931.723 kb on - strandat 3931.762 kb on + strandat 3931.770 kb on - strandat 3931.816 kb on - strandat 3931.816 kb on - strandat 3931.910 kb on - strand, within BWI76_RS18960at 3931.936 kb on + strand, within BWI76_RS18960at 3932.013 kb on + strand, within BWI76_RS18960at 3932.036 kb on + strand, within BWI76_RS18960at 3932.045 kb on - strand, within BWI76_RS18960at 3932.094 kb on + strandat 3932.094 kb on + strand, within BWI76_RS18960at 3932.104 kb on - strand, within BWI76_RS18960at 3932.112 kb on + strand, within BWI76_RS18960at 3932.196 kb on + strand, within BWI76_RS18960at 3932.196 kb on + strand, within BWI76_RS18960at 3932.196 kb on + strand, within BWI76_RS18960at 3932.196 kb on + strand, within BWI76_RS18960at 3932.204 kb on - strand, within BWI76_RS18960at 3932.204 kb on - strand, within BWI76_RS18960at 3932.311 kb on - strand, within BWI76_RS18960at 3932.311 kb on - strand, within BWI76_RS18960at 3932.317 kb on + strand, within BWI76_RS18960at 3932.326 kb on - strand, within BWI76_RS18960at 3932.338 kb on + strand, within BWI76_RS18960at 3932.341 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.346 kb on + strand, within BWI76_RS18960at 3932.354 kb on - strand, within BWI76_RS18960at 3932.354 kb on - strand, within BWI76_RS18960at 3932.355 kb on - strand, within BWI76_RS18960at 3932.444 kb on - strand, within BWI76_RS18960at 3932.463 kb on - strand, within BWI76_RS18960at 3932.463 kb on - strandat 3932.480 kb on + strand, within BWI76_RS18960at 3932.513 kb on - strand, within BWI76_RS18960at 3932.516 kb on + strand, within BWI76_RS18960at 3932.516 kb on + strand, within BWI76_RS18960at 3932.524 kb on - strand, within BWI76_RS18960at 3932.524 kb on - strand, within BWI76_RS18960at 3932.524 kb on - strand, within BWI76_RS18960at 3932.570 kb on + strand, within BWI76_RS18960at 3932.578 kb on - strand, within BWI76_RS18960at 3932.585 kb on - strand, within BWI76_RS18960at 3932.624 kb on + strand, within BWI76_RS18960at 3932.624 kb on + strandat 3932.629 kb on + strand, within BWI76_RS18960at 3932.632 kb on - strand, within BWI76_RS18960at 3932.637 kb on - strand, within BWI76_RS18960

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Per-strain Table

Position Strand Gene LocusTag Fraction Urea (N)
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3,930,057 + BWI76_RS18945 0.68 -1.5
3,930,223 - -2.8
3,930,377 + BWI76_RS18950 0.13 -6.2
3,930,426 + BWI76_RS18950 0.20 -4.0
3,930,445 + BWI76_RS18950 0.22 -5.2
3,930,909 + BWI76_RS18950 0.82 -3.4
3,931,121 + BWI76_RS18955 0.13 -5.4
3,931,498 - BWI76_RS18955 0.76 -3.3
3,931,509 + BWI76_RS18955 0.78 -2.0
3,931,517 + BWI76_RS18955 0.79 -5.7
3,931,517 + BWI76_RS18955 0.79 -3.9
3,931,640 - -0.2
3,931,653 - +0.1
3,931,674 + +0.8
3,931,723 - +0.1
3,931,762 + +0.5
3,931,770 - -0.0
3,931,816 - -1.3
3,931,816 - +0.3
3,931,910 - BWI76_RS18960 0.14 +0.4
3,931,936 + BWI76_RS18960 0.16 -0.8
3,932,013 + BWI76_RS18960 0.22 -0.3
3,932,036 + BWI76_RS18960 0.23 +1.4
3,932,045 - BWI76_RS18960 0.24 -0.3
3,932,094 + -0.9
3,932,094 + BWI76_RS18960 0.28 -0.4
3,932,104 - BWI76_RS18960 0.29 -0.5
3,932,112 + BWI76_RS18960 0.29 +0.7
3,932,196 + BWI76_RS18960 0.36 -1.0
3,932,196 + BWI76_RS18960 0.36 -0.2
3,932,196 + BWI76_RS18960 0.36 +0.1
3,932,196 + BWI76_RS18960 0.36 +0.3
3,932,204 - BWI76_RS18960 0.36 -0.5
3,932,204 - BWI76_RS18960 0.36 -0.2
3,932,311 - BWI76_RS18960 0.44 -0.8
3,932,311 - BWI76_RS18960 0.44 -0.7
3,932,317 + BWI76_RS18960 0.45 +0.6
3,932,326 - BWI76_RS18960 0.45 -0.1
3,932,338 + BWI76_RS18960 0.46 -0.3
3,932,341 + BWI76_RS18960 0.47 +0.7
3,932,346 + BWI76_RS18960 0.47 -0.1
3,932,346 + BWI76_RS18960 0.47 -0.7
3,932,346 + BWI76_RS18960 0.47 -0.1
3,932,346 + BWI76_RS18960 0.47 -0.4
3,932,346 + BWI76_RS18960 0.47 -1.7
3,932,346 + BWI76_RS18960 0.47 +0.0
3,932,354 - BWI76_RS18960 0.48 +0.5
3,932,354 - BWI76_RS18960 0.48 -0.3
3,932,355 - BWI76_RS18960 0.48 +0.3
3,932,444 - BWI76_RS18960 0.54 +0.4
3,932,463 - BWI76_RS18960 0.56 -0.2
3,932,463 - +1.1
3,932,480 + BWI76_RS18960 0.57 -0.6
3,932,513 - BWI76_RS18960 0.60 -0.8
3,932,516 + BWI76_RS18960 0.60 +0.0
3,932,516 + BWI76_RS18960 0.60 -1.4
3,932,524 - BWI76_RS18960 0.60 +1.2
3,932,524 - BWI76_RS18960 0.60 -0.3
3,932,524 - BWI76_RS18960 0.60 -0.3
3,932,570 + BWI76_RS18960 0.64 -1.8
3,932,578 - BWI76_RS18960 0.65 -1.9
3,932,585 - BWI76_RS18960 0.65 +0.1
3,932,624 + BWI76_RS18960 0.68 +0.6
3,932,624 + +0.1
3,932,629 + BWI76_RS18960 0.68 +0.2
3,932,632 - BWI76_RS18960 0.69 -0.4
3,932,637 - BWI76_RS18960 0.69 +0.5

Or see this region's nucleotide sequence