Experiment: 3-Hydroxypropionate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02621 and H281DRAFT_02622 overlap by 4 nucleotides H281DRAFT_02622 and H281DRAFT_02623 overlap by 20 nucleotides H281DRAFT_02623 and H281DRAFT_02624 overlap by 4 nucleotides H281DRAFT_02624 and H281DRAFT_02625 are separated by 18 nucleotides
H281DRAFT_02621: H281DRAFT_02621 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 197,850 to 199,193
_02621
H281DRAFT_02622: H281DRAFT_02622 - hypothetical protein, at 199,190 to 199,357
_02622
H281DRAFT_02623: H281DRAFT_02623 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 199,338 to 200,678
_02623
H281DRAFT_02624: H281DRAFT_02624 - 4-carboxymuconolactone decarboxylase, at 200,675 to 201,229
_02624
H281DRAFT_02625: H281DRAFT_02625 - amino acid ABC transporter substrate-binding protein, PAAT family, at 201,248 to 202,153
_02625
Position (kb) 199
200
201 Strain fitness (log2 ratio) -1
0
1 at 198.759 kb on + strand, within H281DRAFT_02621 at 198.760 kb on - strand, within H281DRAFT_02621 at 198.760 kb on - strand, within H281DRAFT_02621 at 200.011 kb on - strand, within H281DRAFT_02623 at 200.177 kb on + strand, within H281DRAFT_02623 at 200.225 kb on - strand, within H281DRAFT_02623 at 200.325 kb on + strand, within H281DRAFT_02623 at 201.083 kb on - strand, within H281DRAFT_02624 at 201.083 kb on - strand, within H281DRAFT_02624 at 201.360 kb on + strand, within H281DRAFT_02625 at 201.421 kb on + strand, within H281DRAFT_02625 at 201.422 kb on - strand, within H281DRAFT_02625 at 201.552 kb on + strand, within H281DRAFT_02625 at 201.552 kb on + strand, within H281DRAFT_02625 at 201.553 kb on - strand, within H281DRAFT_02625 at 201.553 kb on - strand, within H281DRAFT_02625 at 201.614 kb on - strand, within H281DRAFT_02625 at 201.617 kb on - strand, within H281DRAFT_02625
Per-strain Table
Position Strand Gene LocusTag Fraction 3-Hydroxypropionate (C) remove 198,759 + H281DRAFT_02621 0.68 -0.3 198,760 - H281DRAFT_02621 0.68 +0.4 198,760 - H281DRAFT_02621 0.68 -0.7 200,011 - H281DRAFT_02623 0.50 +0.2 200,177 + H281DRAFT_02623 0.63 +1.0 200,225 - H281DRAFT_02623 0.66 +0.8 200,325 + H281DRAFT_02623 0.74 +0.7 201,083 - H281DRAFT_02624 0.74 +0.0 201,083 - H281DRAFT_02624 0.74 +0.1 201,360 + H281DRAFT_02625 0.12 +0.3 201,421 + H281DRAFT_02625 0.19 +0.0 201,422 - H281DRAFT_02625 0.19 +0.0 201,552 + H281DRAFT_02625 0.34 -0.5 201,552 + H281DRAFT_02625 0.34 -0.2 201,553 - H281DRAFT_02625 0.34 +1.4 201,553 - H281DRAFT_02625 0.34 +0.1 201,614 - H281DRAFT_02625 0.40 +1.1 201,617 - H281DRAFT_02625 0.41 -0.8
Or see this region's nucleotide sequence