Experiment: reconstituted MRS with Vitafiber-prebiotic 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14975 and BBR_RS14980 are separated by 147 nucleotides BBR_RS14980 and BBR_RS14985 are separated by 244 nucleotides
BBR_RS14975: BBR_RS14975 - purine permease, at 1,132,158 to 1,133,522
_RS14975
BBR_RS14980: BBR_RS14980 - xanthine phosphoribosyltransferase, at 1,133,670 to 1,134,251
_RS14980
BBR_RS14985: BBR_RS14985 - ATP-dependent DNA helicase PcrA, at 1,134,496 to 1,137,171
_RS14985
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1 at 1132.692 kb on + strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.722 kb on + strand, within BBR_RS14975 at 1132.733 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.768 kb on - strand, within BBR_RS14975 at 1132.768 kb on - strand, within BBR_RS14975 at 1132.836 kb on + strand, within BBR_RS14975 at 1132.872 kb on + strand, within BBR_RS14975 at 1132.910 kb on - strand, within BBR_RS14975 at 1133.017 kb on - strand, within BBR_RS14975 at 1133.055 kb on - strand, within BBR_RS14975 at 1133.170 kb on + strand, within BBR_RS14975 at 1133.185 kb on + strand, within BBR_RS14975 at 1133.185 kb on + strand, within BBR_RS14975 at 1133.193 kb on - strand, within BBR_RS14975 at 1133.242 kb on + strand, within BBR_RS14975 at 1133.256 kb on - strand, within BBR_RS14975 at 1133.286 kb on - strand, within BBR_RS14975 at 1133.288 kb on + strand, within BBR_RS14975 at 1133.289 kb on - strand, within BBR_RS14975 at 1133.296 kb on - strand, within BBR_RS14975 at 1133.343 kb on - strand, within BBR_RS14975 at 1133.358 kb on + strand, within BBR_RS14975 at 1133.361 kb on - strand, within BBR_RS14975 at 1133.544 kb on + strand at 1133.564 kb on - strand at 1133.587 kb on + strand at 1133.587 kb on - strand at 1133.648 kb on - strand at 1133.673 kb on - strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.680 kb on + strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.794 kb on + strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.928 kb on + strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.990 kb on + strand, within BBR_RS14980 at 1134.029 kb on - strand, within BBR_RS14980 at 1134.182 kb on + strand, within BBR_RS14980 at 1134.213 kb on - strand at 1134.231 kb on - strand at 1134.256 kb on + strand at 1134.259 kb on + strand at 1134.314 kb on - strand at 1134.420 kb on - strand at 1134.420 kb on - strand at 1134.425 kb on - strand at 1134.425 kb on - strand at 1134.441 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Vitafiber-prebiotic 0.5 %w/v remove 1,132,692 + BBR_RS14975 0.39 -0.5 1,132,700 - BBR_RS14975 0.40 -0.6 1,132,700 - BBR_RS14975 0.40 +0.8 1,132,700 - BBR_RS14975 0.40 +0.5 1,132,722 + BBR_RS14975 0.41 +0.2 1,132,733 + BBR_RS14975 0.42 -0.7 1,132,750 + BBR_RS14975 0.43 -0.1 1,132,750 + BBR_RS14975 0.43 -1.8 1,132,750 + BBR_RS14975 0.43 +1.4 1,132,758 - BBR_RS14975 0.44 +0.2 1,132,758 - BBR_RS14975 0.44 +0.1 1,132,758 - BBR_RS14975 0.44 +0.7 1,132,768 - BBR_RS14975 0.45 +0.3 1,132,768 - BBR_RS14975 0.45 -2.2 1,132,836 + BBR_RS14975 0.50 +0.7 1,132,872 + BBR_RS14975 0.52 -0.1 1,132,910 - BBR_RS14975 0.55 -0.4 1,133,017 - BBR_RS14975 0.63 +0.0 1,133,055 - BBR_RS14975 0.66 +0.9 1,133,170 + BBR_RS14975 0.74 +1.7 1,133,185 + BBR_RS14975 0.75 +1.0 1,133,185 + BBR_RS14975 0.75 -0.2 1,133,193 - BBR_RS14975 0.76 -0.7 1,133,242 + BBR_RS14975 0.79 -0.2 1,133,256 - BBR_RS14975 0.80 +1.6 1,133,286 - BBR_RS14975 0.83 -0.8 1,133,288 + BBR_RS14975 0.83 -0.1 1,133,289 - BBR_RS14975 0.83 -2.7 1,133,296 - BBR_RS14975 0.83 +0.1 1,133,343 - BBR_RS14975 0.87 +0.5 1,133,358 + BBR_RS14975 0.88 -0.4 1,133,361 - BBR_RS14975 0.88 -0.0 1,133,544 + +0.8 1,133,564 - -0.8 1,133,587 + -0.2 1,133,587 - +0.9 1,133,648 - -0.2 1,133,673 - +0.2 1,133,675 + -0.8 1,133,675 + +0.5 1,133,675 + -0.0 1,133,675 + -0.1 1,133,680 + -0.3 1,133,683 - +0.4 1,133,683 - +0.0 1,133,683 - -0.1 1,133,794 + BBR_RS14980 0.21 +1.0 1,133,832 - BBR_RS14980 0.28 -1.4 1,133,832 - BBR_RS14980 0.28 +0.2 1,133,928 + BBR_RS14980 0.44 +0.6 1,133,953 - BBR_RS14980 0.49 -0.0 1,133,953 - BBR_RS14980 0.49 -0.3 1,133,955 + BBR_RS14980 0.49 -0.4 1,133,955 + BBR_RS14980 0.49 -0.4 1,133,990 + BBR_RS14980 0.55 -0.3 1,134,029 - BBR_RS14980 0.62 -0.7 1,134,182 + BBR_RS14980 0.88 -0.2 1,134,213 - -0.3 1,134,231 - +0.2 1,134,256 + -0.2 1,134,259 + -0.5 1,134,314 - +0.2 1,134,420 - +0.2 1,134,420 - -1.9 1,134,425 - +1.1 1,134,425 - -0.2 1,134,441 + -0.3
Or see this region's nucleotide sequence