Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2577 and Dshi_2578 are separated by 260 nucleotides Dshi_2578 and Dshi_2579 are separated by 143 nucleotides Dshi_2579 and Dshi_2580 are separated by 165 nucleotides
Dshi_2577: Dshi_2577 - putative RNA methylase (RefSeq), at 2,742,482 to 2,743,606
_2577
Dshi_2578: Dshi_2578 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (RefSeq), at 2,743,867 to 2,744,589
_2578
Dshi_2579: Dshi_2579 - hypothetical protein (RefSeq), at 2,744,733 to 2,745,137
_2579
Dshi_2580: Dshi_2580 - ATP-dependent helicase HrpB (RefSeq), at 2,745,303 to 2,747,807
_2580
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2742.956 kb on - strand, within Dshi_2577 at 2743.133 kb on - strand, within Dshi_2577 at 2743.255 kb on + strand, within Dshi_2577 at 2743.311 kb on + strand, within Dshi_2577 at 2743.373 kb on - strand, within Dshi_2577 at 2743.447 kb on + strand, within Dshi_2577 at 2743.498 kb on + strand at 2743.521 kb on - strand at 2743.629 kb on + strand at 2743.632 kb on - strand at 2743.638 kb on - strand at 2743.690 kb on + strand at 2743.698 kb on - strand at 2743.741 kb on + strand at 2743.855 kb on + strand at 2744.681 kb on - strand at 2744.810 kb on + strand, within Dshi_2579 at 2744.825 kb on + strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.945 kb on + strand, within Dshi_2579 at 2744.985 kb on - strand, within Dshi_2579 at 2745.115 kb on + strand at 2745.123 kb on - strand at 2745.123 kb on - strand at 2745.177 kb on + strand at 2745.308 kb on - strand at 2745.335 kb on - strand at 2745.384 kb on + strand at 2745.414 kb on - strand at 2745.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 2,742,956 - Dshi_2577 0.42 -0.3 2,743,133 - Dshi_2577 0.58 -0.8 2,743,255 + Dshi_2577 0.69 -0.6 2,743,311 + Dshi_2577 0.74 +1.0 2,743,373 - Dshi_2577 0.79 -1.2 2,743,447 + Dshi_2577 0.86 +0.1 2,743,498 + -0.5 2,743,521 - +0.6 2,743,629 + +0.2 2,743,632 - -1.0 2,743,638 - +0.6 2,743,690 + +2.8 2,743,698 - +0.1 2,743,741 + -1.2 2,743,855 + -0.9 2,744,681 - +0.6 2,744,810 + Dshi_2579 0.19 +0.7 2,744,825 + Dshi_2579 0.23 +0.3 2,744,833 - Dshi_2579 0.25 +0.3 2,744,833 - Dshi_2579 0.25 -0.5 2,744,833 - Dshi_2579 0.25 -0.3 2,744,942 - Dshi_2579 0.52 +1.0 2,744,942 - Dshi_2579 0.52 -0.8 2,744,945 + Dshi_2579 0.52 -0.5 2,744,985 - Dshi_2579 0.62 -0.0 2,745,115 + +0.5 2,745,123 - -0.9 2,745,123 - +0.8 2,745,177 + -0.3 2,745,308 - +0.3 2,745,335 - -0.4 2,745,384 + +0.5 2,745,414 - -0.3 2,745,515 + -1.5
Or see this region's nucleotide sequence