Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2575 and Dshi_2576 overlap by 4 nucleotides Dshi_2576 and Dshi_2577 are separated by 100 nucleotides Dshi_2577 and Dshi_2578 are separated by 260 nucleotides
Dshi_2575: Dshi_2575 - phosphoribosyl-ATP pyrophosphohydrolase (RefSeq), at 2,741,315 to 2,741,623
_2575
Dshi_2576: Dshi_2576 - imidazoleglycerol phosphate synthase, cyclase subunit (RefSeq), at 2,741,620 to 2,742,381
_2576
Dshi_2577: Dshi_2577 - putative RNA methylase (RefSeq), at 2,742,482 to 2,743,606
_2577
Dshi_2578: Dshi_2578 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (RefSeq), at 2,743,867 to 2,744,589
_2578
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2742.564 kb on + strand at 2742.619 kb on - strand, within Dshi_2577 at 2742.704 kb on + strand, within Dshi_2577 at 2742.854 kb on + strand, within Dshi_2577 at 2742.956 kb on - strand, within Dshi_2577 at 2743.133 kb on - strand, within Dshi_2577 at 2743.255 kb on + strand, within Dshi_2577 at 2743.311 kb on + strand, within Dshi_2577 at 2743.373 kb on - strand, within Dshi_2577 at 2743.447 kb on + strand, within Dshi_2577 at 2743.498 kb on + strand at 2743.521 kb on - strand at 2743.629 kb on + strand at 2743.632 kb on - strand at 2743.638 kb on - strand at 2743.690 kb on + strand at 2743.698 kb on - strand at 2743.741 kb on + strand at 2743.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 2,742,564 + -1.8 2,742,619 - Dshi_2577 0.12 -0.4 2,742,704 + Dshi_2577 0.20 +1.8 2,742,854 + Dshi_2577 0.33 +0.1 2,742,956 - Dshi_2577 0.42 -0.3 2,743,133 - Dshi_2577 0.58 -0.8 2,743,255 + Dshi_2577 0.69 -0.6 2,743,311 + Dshi_2577 0.74 +1.0 2,743,373 - Dshi_2577 0.79 -1.2 2,743,447 + Dshi_2577 0.86 +0.1 2,743,498 + -0.5 2,743,521 - +0.6 2,743,629 + +0.2 2,743,632 - -1.0 2,743,638 - +0.6 2,743,690 + +2.8 2,743,698 - +0.1 2,743,741 + -1.2 2,743,855 + -0.9
Or see this region's nucleotide sequence