Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2321 and Dshi_2322 are separated by 23 nucleotides Dshi_2322 and Dshi_2323 are separated by 11 nucleotides Dshi_2323 and Dshi_2324 are separated by 90 nucleotides Dshi_2324 and Dshi_2325 are separated by 95 nucleotides
Dshi_2321: Dshi_2321 - DinB family protein (RefSeq), at 2,453,285 to 2,453,764
_2321
Dshi_2322: Dshi_2322 - protein of unknown function DUF1326 (RefSeq), at 2,453,788 to 2,454,489
_2322
Dshi_2323: Dshi_2323 - hypothetical protein (RefSeq), at 2,454,501 to 2,455,259
_2323
Dshi_2324: Dshi_2324 - regulatory protein GntR HTH (RefSeq), at 2,455,350 to 2,456,078
_2324
Dshi_2325: Dshi_2325 - spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein (RefSeq), at 2,456,174 to 2,457,220
_2325
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2453.532 kb on + strand, within Dshi_2321 at 2453.537 kb on + strand, within Dshi_2321 at 2453.540 kb on - strand, within Dshi_2321 at 2453.552 kb on - strand, within Dshi_2321 at 2453.584 kb on - strand, within Dshi_2321 at 2453.589 kb on + strand, within Dshi_2321 at 2453.589 kb on + strand, within Dshi_2321 at 2453.633 kb on - strand, within Dshi_2321 at 2453.704 kb on + strand, within Dshi_2321 at 2453.786 kb on + strand at 2453.824 kb on - strand at 2453.859 kb on - strand, within Dshi_2322 at 2453.910 kb on - strand, within Dshi_2322 at 2453.910 kb on - strand, within Dshi_2322 at 2454.061 kb on - strand, within Dshi_2322 at 2454.087 kb on - strand, within Dshi_2322 at 2454.087 kb on - strand, within Dshi_2322 at 2454.095 kb on + strand, within Dshi_2322 at 2454.291 kb on - strand, within Dshi_2322 at 2454.316 kb on - strand, within Dshi_2322 at 2454.343 kb on + strand, within Dshi_2322 at 2454.346 kb on + strand, within Dshi_2322 at 2454.456 kb on + strand at 2454.471 kb on + strand at 2454.477 kb on - strand at 2454.479 kb on + strand at 2454.487 kb on - strand at 2454.508 kb on - strand at 2454.530 kb on + strand at 2454.546 kb on + strand at 2454.573 kb on - strand at 2454.583 kb on - strand, within Dshi_2323 at 2454.663 kb on + strand, within Dshi_2323 at 2454.685 kb on - strand, within Dshi_2323 at 2454.802 kb on + strand, within Dshi_2323 at 2454.850 kb on + strand, within Dshi_2323 at 2454.850 kb on - strand, within Dshi_2323 at 2454.859 kb on - strand, within Dshi_2323 at 2454.864 kb on - strand, within Dshi_2323 at 2454.883 kb on - strand, within Dshi_2323 at 2454.898 kb on + strand, within Dshi_2323 at 2454.930 kb on - strand, within Dshi_2323 at 2454.971 kb on - strand, within Dshi_2323 at 2454.981 kb on - strand, within Dshi_2323 at 2454.986 kb on - strand, within Dshi_2323 at 2455.019 kb on + strand, within Dshi_2323 at 2455.036 kb on - strand, within Dshi_2323 at 2455.048 kb on - strand, within Dshi_2323 at 2455.129 kb on + strand, within Dshi_2323 at 2455.156 kb on - strand, within Dshi_2323 at 2455.260 kb on - strand at 2455.260 kb on - strand at 2455.263 kb on - strand at 2455.263 kb on - strand at 2455.346 kb on - strand at 2455.371 kb on + strand at 2455.411 kb on - strand at 2455.418 kb on + strand at 2455.527 kb on + strand, within Dshi_2324 at 2455.563 kb on + strand, within Dshi_2324 at 2455.596 kb on - strand, within Dshi_2324 at 2455.635 kb on - strand, within Dshi_2324 at 2455.635 kb on - strand, within Dshi_2324 at 2455.689 kb on + strand, within Dshi_2324 at 2455.694 kb on + strand, within Dshi_2324 at 2455.816 kb on + strand, within Dshi_2324 at 2455.817 kb on - strand, within Dshi_2324 at 2455.840 kb on - strand, within Dshi_2324 at 2455.869 kb on - strand, within Dshi_2324 at 2455.972 kb on + strand, within Dshi_2324 at 2456.006 kb on + strand at 2456.009 kb on - strand at 2456.046 kb on + strand at 2456.091 kb on - strand at 2456.125 kb on + strand at 2456.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 2,453,532 + Dshi_2321 0.51 -0.6 2,453,537 + Dshi_2321 0.53 -0.3 2,453,540 - Dshi_2321 0.53 -0.6 2,453,552 - Dshi_2321 0.56 -0.4 2,453,584 - Dshi_2321 0.62 +0.5 2,453,589 + Dshi_2321 0.63 +0.6 2,453,589 + Dshi_2321 0.63 +1.2 2,453,633 - Dshi_2321 0.72 -1.0 2,453,704 + Dshi_2321 0.87 -0.2 2,453,786 + -0.6 2,453,824 - -0.9 2,453,859 - Dshi_2322 0.10 -1.4 2,453,910 - Dshi_2322 0.17 -0.0 2,453,910 - Dshi_2322 0.17 -1.5 2,454,061 - Dshi_2322 0.39 +0.0 2,454,087 - Dshi_2322 0.43 +0.8 2,454,087 - Dshi_2322 0.43 +0.2 2,454,095 + Dshi_2322 0.44 -0.3 2,454,291 - Dshi_2322 0.72 -1.2 2,454,316 - Dshi_2322 0.75 -0.8 2,454,343 + Dshi_2322 0.79 +0.4 2,454,346 + Dshi_2322 0.79 -0.6 2,454,456 + -1.8 2,454,471 + -0.4 2,454,477 - +2.8 2,454,479 + +0.9 2,454,487 - -1.0 2,454,508 - +0.8 2,454,530 + -1.5 2,454,546 + -0.2 2,454,573 - -0.2 2,454,583 - Dshi_2323 0.11 -0.5 2,454,663 + Dshi_2323 0.21 -0.2 2,454,685 - Dshi_2323 0.24 +0.2 2,454,802 + Dshi_2323 0.40 -0.9 2,454,850 + Dshi_2323 0.46 -0.1 2,454,850 - Dshi_2323 0.46 -1.5 2,454,859 - Dshi_2323 0.47 +0.5 2,454,864 - Dshi_2323 0.48 -0.7 2,454,883 - Dshi_2323 0.50 +2.1 2,454,898 + Dshi_2323 0.52 -0.3 2,454,930 - Dshi_2323 0.57 +0.4 2,454,971 - Dshi_2323 0.62 -0.1 2,454,981 - Dshi_2323 0.63 +0.5 2,454,986 - Dshi_2323 0.64 +0.2 2,455,019 + Dshi_2323 0.68 -0.1 2,455,036 - Dshi_2323 0.70 -0.0 2,455,048 - Dshi_2323 0.72 -0.5 2,455,129 + Dshi_2323 0.83 -0.5 2,455,156 - Dshi_2323 0.86 +0.2 2,455,260 - -2.0 2,455,260 - -0.4 2,455,263 - +1.1 2,455,263 - -0.5 2,455,346 - +0.1 2,455,371 + -0.7 2,455,411 - -0.4 2,455,418 + +0.5 2,455,527 + Dshi_2324 0.24 -0.3 2,455,563 + Dshi_2324 0.29 -0.5 2,455,596 - Dshi_2324 0.34 -1.5 2,455,635 - Dshi_2324 0.39 -0.4 2,455,635 - Dshi_2324 0.39 +0.1 2,455,689 + Dshi_2324 0.47 -0.9 2,455,694 + Dshi_2324 0.47 -0.2 2,455,816 + Dshi_2324 0.64 -1.4 2,455,817 - Dshi_2324 0.64 +0.9 2,455,840 - Dshi_2324 0.67 -0.2 2,455,869 - Dshi_2324 0.71 -1.1 2,455,972 + Dshi_2324 0.85 -1.0 2,456,006 + +0.5 2,456,009 - -1.0 2,456,046 + -2.2 2,456,091 - -0.2 2,456,125 + -1.1 2,456,183 + +1.2
Or see this region's nucleotide sequence