Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1928 and xerC are separated by 3 nucleotides xerC and Dshi_1930 are separated by 19 nucleotides Dshi_1930 and Dshi_1931 are separated by 62 nucleotides Dshi_1931 and Dshi_1932 overlap by 4 nucleotides
Dshi_1928: Dshi_1928 - protein of unknown function DUF484 (RefSeq), at 2,015,542 to 2,016,249
_1928
Dshi_5002: xerC - hypothetical protein (NCBI), at 2,016,253 to 2,017,179
xerC
Dshi_1930: Dshi_1930 - protein of unknown function DUF6 transmembrane (RefSeq), at 2,017,199 to 2,018,038
_1930
Dshi_1931: Dshi_1931 - hypothetical protein (RefSeq), at 2,018,101 to 2,018,352
_1931
Dshi_1932: Dshi_1932 - hypothetical protein (RefSeq), at 2,018,349 to 2,019,218
_1932
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-1
0
1
2 at 2016.345 kb on + strand at 2017.121 kb on - strand at 2017.121 kb on - strand at 2017.160 kb on - strand at 2017.173 kb on + strand at 2017.181 kb on - strand at 2017.221 kb on - strand at 2017.573 kb on - strand, within Dshi_1930 at 2017.619 kb on + strand, within Dshi_1930 at 2017.838 kb on - strand, within Dshi_1930 at 2018.082 kb on - strand at 2018.105 kb on - strand at 2018.130 kb on + strand, within Dshi_1931 at 2018.341 kb on + strand at 2018.612 kb on - strand, within Dshi_1932 at 2018.761 kb on - strand, within Dshi_1932 at 2018.815 kb on - strand, within Dshi_1932 at 2018.904 kb on + strand, within Dshi_1932
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 2,016,345 + +1.9 2,017,121 - -0.0 2,017,121 - -1.3 2,017,160 - -0.0 2,017,173 + +0.3 2,017,181 - +0.2 2,017,221 - +0.5 2,017,573 - Dshi_1930 0.45 -1.3 2,017,619 + Dshi_1930 0.50 -0.5 2,017,838 - Dshi_1930 0.76 -1.0 2,018,082 - -0.1 2,018,105 - +0.4 2,018,130 + Dshi_1931 0.12 -1.1 2,018,341 + -0.9 2,018,612 - Dshi_1932 0.30 -1.0 2,018,761 - Dshi_1932 0.47 -0.4 2,018,815 - Dshi_1932 0.54 +0.9 2,018,904 + Dshi_1932 0.64 -0.4
Or see this region's nucleotide sequence