Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1639 and Dshi_1640 overlap by 4 nucleotides Dshi_1640 and Dshi_1641 are separated by 12 nucleotides Dshi_1641 and Dshi_1642 are separated by 4 nucleotides Dshi_1642 and Dshi_1643 are separated by 144 nucleotides
Dshi_1639: Dshi_1639 - hypothetical protein (RefSeq), at 1,700,730 to 1,701,272
_1639
Dshi_1640: Dshi_1640 - hypothetical protein (RefSeq), at 1,701,269 to 1,701,574
_1640
Dshi_1641: Dshi_1641 - protein of unknown function DUF1330 (RefSeq), at 1,701,587 to 1,701,880
_1641
Dshi_1642: Dshi_1642 - alanyl-tRNA synthetase (RefSeq), at 1,701,885 to 1,704,548
_1642
Dshi_1643: Dshi_1643 - recA protein (RefSeq), at 1,704,693 to 1,705,760
_1643
Position (kb)
1701
1702
1703
1704
1705 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1700.922 kb on - strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.275 kb on - strand at 1701.515 kb on - strand, within Dshi_1640 at 1701.647 kb on + strand, within Dshi_1641 at 1701.746 kb on - strand, within Dshi_1641 at 1704.602 kb on + strand at 1704.602 kb on + strand at 1704.610 kb on - strand at 1704.610 kb on - strand at 1704.789 kb on - strand at 1704.885 kb on - strand, within Dshi_1643 at 1704.988 kb on + strand, within Dshi_1643 at 1705.105 kb on - strand, within Dshi_1643 at 1705.316 kb on + strand, within Dshi_1643 at 1705.504 kb on - strand, within Dshi_1643 at 1705.520 kb on + strand, within Dshi_1643
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 1,700,922 - Dshi_1639 0.35 +0.7 1,701,132 + Dshi_1639 0.74 -0.0 1,701,132 + Dshi_1639 0.74 -0.2 1,701,140 - Dshi_1639 0.76 -0.2 1,701,140 - Dshi_1639 0.76 +0.2 1,701,275 - -1.3 1,701,515 - Dshi_1640 0.80 -0.2 1,701,647 + Dshi_1641 0.20 +0.4 1,701,746 - Dshi_1641 0.54 +0.2 1,704,602 + +0.8 1,704,602 + -0.4 1,704,610 - -0.7 1,704,610 - -0.9 1,704,789 - -1.4 1,704,885 - Dshi_1643 0.18 -0.5 1,704,988 + Dshi_1643 0.28 -2.4 1,705,105 - Dshi_1643 0.39 -2.0 1,705,316 + Dshi_1643 0.58 +0.8 1,705,504 - Dshi_1643 0.76 -3.2 1,705,520 + Dshi_1643 0.77 -1.0
Or see this region's nucleotide sequence