Experiment: m.b. Nalidixic 0.048 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0976 and Dshi_R0022 are separated by 197 nucleotides Dshi_R0022 and Dshi_0977 are separated by 294 nucleotides Dshi_0977 and Dshi_0978 overlap by 4 nucleotides Dshi_0978 and Dshi_0979 are separated by 60 nucleotides
Dshi_0976: Dshi_0976 - resolvase-like protein (RefSeq), at 997,950 to 998,168
_0976
Dshi_R0022: Dshi_R0022 - tRNA-Thr (RefSeq), at 998,366 to 998,440
_R0022
Dshi_0977: Dshi_0977 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (RefSeq), at 998,735 to 1,000,003
_0977
Dshi_0978: Dshi_0978 - hypothetical protein (RefSeq), at 1,000,000 to 1,000,476
_0978
Dshi_0979: Dshi_0979 - Histidinol dehydrogenase (RefSeq), at 1,000,537 to 1,001,853
_0979
Position (kb) 998
999
1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 997.805 kb on + strand at 997.846 kb on + strand at 997.847 kb on + strand at 997.855 kb on - strand at 997.857 kb on + strand at 997.865 kb on - strand at 997.865 kb on - strand at 997.875 kb on - strand at 997.960 kb on + strand at 997.995 kb on - strand, within Dshi_0976 at 998.019 kb on + strand, within Dshi_0976 at 998.089 kb on + strand, within Dshi_0976 at 998.231 kb on + strand at 998.231 kb on + strand at 998.256 kb on - strand at 998.282 kb on - strand at 998.305 kb on - strand at 998.534 kb on - strand at 998.535 kb on - strand at 998.535 kb on - strand at 998.535 kb on - strand at 998.546 kb on - strand at 998.590 kb on - strand at 1000.124 kb on - strand, within Dshi_0978 at 1000.144 kb on - strand, within Dshi_0978 at 1000.186 kb on - strand, within Dshi_0978 at 1000.271 kb on + strand, within Dshi_0978 at 1000.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.048 mg/ml remove 997,805 + +1.0 997,846 + -1.3 997,847 + +0.5 997,855 - -0.8 997,857 + -3.0 997,865 - -1.1 997,865 - -0.7 997,875 - -0.9 997,960 + +0.4 997,995 - Dshi_0976 0.21 -0.8 998,019 + Dshi_0976 0.32 -0.5 998,089 + Dshi_0976 0.63 -0.6 998,231 + -0.7 998,231 + -0.1 998,256 - +0.6 998,282 - -1.8 998,305 - -1.5 998,534 - -1.7 998,535 - +0.1 998,535 - +1.0 998,535 - +0.0 998,546 - -0.8 998,590 - -0.3 1,000,124 - Dshi_0978 0.26 -0.7 1,000,144 - Dshi_0978 0.30 -0.5 1,000,186 - Dshi_0978 0.39 -1.1 1,000,271 + Dshi_0978 0.57 -0.7 1,000,439 + -0.5
Or see this region's nucleotide sequence